Role of plasma membrane tension in actin polymerization

Research Project

Project/Area Number	25440085
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Cell biology
Research Institution	Kobe University
Principal Investigator	Tsujita Kazuya 神戸大学, バイオシグナル総合研究センター, 講師 (10457054)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	細胞膜の張力 / アクチン細胞骨格 / BARタンパク質 / F-BARタンパク質
Outline of Final Research Achievements	Tension applied to the plasma membrane (PM) is a global mechanical parameter that regulates actin polymerization. However, how membrane tension regulates actin assembly was unknown. This work showed that FBP17, a membrane-bending protein and an activator of WASP/N-WASP-dependent actin nucleation, is a PM tension-sensor involved in leading edge formation. Cell biological experiments showed that membrane tension inhibits membrane bending activity of FBP17 to negatively regulate actin polymerization.

Report

(4 results)