The function of YIPFs, five-span transmembrane proteins localizing in the Golgi apparatus.
| Project/Area Number |
25440092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIDA Ryuichi 京都産業大学, 総合生命科学部・プロジェクト, ポストドクター (30611256)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ゴルジ体 / 小胞輸送 / 膜貫通タンパク質 / 糖鎖 / Brefeldin A / PAS染色 / 幕貫通タンパク質 / ERAD / リソソーム / Yipドメインファミリー / 小胞体 / エンドソーム / 膜貫通部位 |
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| Outline of Final Research Achievements |
Yip1p and Yif1p are multi-span trans-membrane proteins reported to function in the vesicular transport between the ER and the Golgi apparatus. Nine homologues were found in mammalian cells although the functions of those proteins remain largely unknown. Especially, the localization and the function of YIPF1, YIPF2 and YIPF6 have never been analyzed. Immunofluorescence analyses revealed that YIPF1, YIPF2 and YIPF6 localize in trans-Golgi. In addition, gene knockdown and drug treatment analyses showed that YIPF1 and YIPF2 support the assembly of the Golgi apparatus and facilitate the glycosylation in mucin secretory cells.
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Report
(4 results)
Research Products
(6 results)
