The function of YIPFs, five-span transmembrane proteins localizing in the Golgi apparatus.

• Project/Area Number: 25440092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto Sangyo University
• Principal Investigator: NAKAMURA Nobuhiro 京都産業大学, 総合生命科学部, 教授 (50294955)
• Co-Investigator(Renkei-kenkyūsha): ISHIDA Ryuichi 京都産業大学, 総合生命科学部・プロジェクト, ポストドクター (30611256)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ゴルジ体 / 小胞輸送 / 膜貫通タンパク質 / 糖鎖 / Brefeldin A / PAS染色 / 幕貫通タンパク質 / ERAD / リソソーム / Yipドメインファミリー / 小胞体 / エンドソーム / 膜貫通部位

Outline of Final Research Achievements

Yip1p and Yif1p are multi-span trans-membrane proteins reported to function in the vesicular transport between the ER and the Golgi apparatus. Nine homologues were found in mammalian cells although the functions of those proteins remain largely unknown. Especially, the localization and the function of YIPF1, YIPF2 and YIPF6 have never been analyzed. Immunofluorescence analyses revealed that YIPF1, YIPF2 and YIPF6 localize in trans-Golgi. In addition, gene knockdown and drug treatment analyses showed that YIPF1 and YIPF2 support the assembly of the Golgi apparatus and facilitate the glycosylation in mucin secretory cells.

Research Products

• [Int'l Joint Research] University of Leeds(英国)
• [Journal Article] GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations. (2015)
  • Author(s): Ishida R, Yamamoto A, Nakayama K, Sohda M, Misumi Y, Yasunaga T, Nakamura N.
  • Journal Title: FEBS J. Volume: 印刷中 Issue: 11 Pages: 2232-2244
  • DOI: 10.1111/febs.13271
  • Peer Reviewed
• [Presentation] トランス・ゴルジに局在するYIPF群の機能解析 (2016)
  • Author(s): Jeerawat Soonthornsit, Nobuhiro Nakamura
  • Organizer: 第68回日本細胞生物学会大会
  • Place of Presentation: 京都テルサ（京都府・京都市）
  • Year and Date: 2016-06-15
• [Presentation] Rab1のGM130結合とその生理的意義 (2014)
  • Author(s): 石田竜一，安永卓生，山本章嗣，中山和久，吉村信一郎，原田彰宏，中村暢宏
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂・東大寺総合文化センター（奈良市）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] Low cytoplasmic pH reduced ER-Golgi transport and induces disassembly of the Golgi apparatus. (2014)
  • Author(s): Jeerawat Soonthornsit, Nobuhiro Nakamura
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂・東大寺総合文化センター（奈良市）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] Yip1 domain family members localizing in the trans-Golgi/ trans- Golgi network. (2013)
  • Author(s): Jeerawat Soonthornsit, Ryuichi Ishida, Nobuhiro Nakamura.
  • Organizer: Gordon Research Conference
  • Place of Presentation: Proctor Academy, Andover, NH, USA.