| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Polarized epithelial cells organize characteristic array of non-centrosomal microtubules that align along apical to basal axis of the cells. The minus-ends of these microtubules face apically, and the plus-ends face towards the basal side. However, how this orientation is established remains elusive. CAMSAP3 is a member of CAMSAP (Calmodulin-regulated-spectrin-associated protein) family proteins that are known as microtubule minus-end binding proteins at non-centrosomal site. In this study, using CAMSAP3 mutant mice and 3D-cultured Caco-2 cells, we showed that CAMSAP3 localizes to the sub-apical region and tethers the minus-ends of microtubules to this site. In the absence of CAMSAP3 function, the orientation of cytoplasmic microtubules or positioning of cell organelle is perturbed. Obtained results demonstrate that apically localized CAMSAP3 determines the proper orientation of microtubules, and in turn that of cell organelles, in mammalian intestinal epithelial cells.
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