| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To understand the spatio-temporal dynamics of cellular functions in complex multi-cellular systems, we have developed a variety of fluorescent indicators for live cell imaging. The indicators include a Fucci probe that resolves a cell cycle into 7 fractions (CC (Cell-Cycle) Rainbow), oxidative stress probes, and carbohydrate metabolism probes. Practical use of these probes gave us opportunities to realize “cell heterogeneity” and appreciate “cell personality” in many cell culture systems. Our forthcoming studies will focus on generation of flox mouse lines of the probes that allow us to better understand the spatio-temporal regulations of cellular functions in a more physiological context.
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