• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Visualization of cell fates using degron based fluorescence probes.

Research Project

Project/Area Number 25440095
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

SAKAUE ASAKO  国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords多細胞社会 / 生命現象可視化 / デグロン / 蛍光タンパク / Fucci / Fucci / 蛍光性プローブ / 可視化 / 運命決定スイッチ / 蛍光プローブ
Outline of Final Research Achievements

To understand the spatio-temporal dynamics of cellular functions in complex multi-cellular systems, we have developed a variety of fluorescent indicators for live cell imaging. The indicators include a Fucci probe that resolves a cell cycle into 7 fractions (CC (Cell-Cycle) Rainbow), oxidative stress probes, and carbohydrate metabolism probes. Practical use of these probes gave us opportunities to realize “cell heterogeneity” and appreciate “cell personality” in many cell culture systems. Our forthcoming studies will focus on generation of flox mouse lines of the probes that allow us to better understand the spatio-temporal regulations of cellular functions in a more physiological context.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Invited: 7 results) Remarks (2 results)

  • [Journal Article] Involvement of Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-induced Incomplete Cytokinesis in the Polyploidization of Osteoclasts.2016

    • Author(s)
      Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291 Issue: 7 Pages: 3439-3454

    • DOI

      10.1074/jbc.m115.677427

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proliferation-coupled osteoclast differentiation by RANKL: Cell density as a determinant of osteoclast formation.2015

    • Author(s)
      Motiur Rahman M, Takeshita S, Matsuoka K, Kaneko K, Naoe Y, Sakaue-Sawano A, Miyawaki A, Ikeda K.
    • Journal Title

      Bone.

      Volume: 81 Pages: 392-399

    • DOI

      10.1016/j.bone.2015.08.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fucci-guided purification of hematopoietic stem cells with high repopulating activity.2015

    • Author(s)
      Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, *Miyoshi H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 457 Issue: 1 Pages: 7-11

    • DOI

      10.1016/j.bbrc.2014.12.074

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time tracking of cell cycle progression during CD8(+) effector and memory T-cell differentiation.2015

    • Author(s)
      Kinjyo I, Qin J, Tan SY, Wellard CJ, Mrass P, Ritchie W, Doi A, Cavanagh LL, Tomura M, Sakaue-Sawano A, Kanagawa O, Miyawaki A, Hodgkin PD, Weninger W.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 6301-6301

    • DOI

      10.1038/ncomms7301

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualizing the cell-cycle progression of endothelial cells in zebrafish.2014

    • Author(s)
      Fukuhara S (Co-corresponding author), Zhang J, Yuge S, Ando K, Wakayama Y, Sakaue-Sawano A, Miyawaki A, Mochizuki N.
    • Journal Title

      Dev. Biol.

      Volume: 393 Issue: 1 Pages: 10-23

    • DOI

      10.1016/j.ydbio.2014.06.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.2014

    • Author(s)
      Mort RL, Ford MJ, Sakaue-Sawano A, Lindstrom NO, Casadio A, Douglas AT, Keighren MA, Hohenstein P, Miyawaki A, *Jackson IJ.
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 17 Pages: 2681-2696

    • DOI

      10.4161/15384101.2015.945381

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel cell-cycle indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition.2014

    • Author(s)
      Oki, T., Nishimura, K., Kitaura, J., Togami, K., Maehara, A., Izawa, K., Sakaue-Sawano. A., Niida, A., Miyano, S., Aburatani, H., Kiyonari, H., Miyawaki, A. and Kitamura, T.
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 4012-4012

    • DOI

      10.1038/srep04012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators2013

    • Author(s)
      Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H, Miyawaki A.
    • Journal Title

      Development

      Volume: 140 Issue: 22 Pages: 4624-4632

    • DOI

      10.1242/dev.099226

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System.2013

    • Author(s)
      Tomura M, Sakaue-Sawano A, Mori Y, Takase-Utsugi M, Hata A, Ohtawa K, Kanagawa O, Miyawaki A.
    • Journal Title

      Plos One

      Volume: 8 Issue: 9 Pages: e73801-e73801

    • DOI

      10.1371/journal.pone.0073801

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 細胞社会のheterogeneityを視る技術2015

    • Author(s)
      阪上-沢野朝子
    • Organizer
      新学術領域ナノメディシン分子科学主催イメージングセミナー
    • Place of Presentation
      東北大学医学部1号館 第1講義室・仙台市・宮城県
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Observing heterogeneity in cell society2014

    • Author(s)
      Asako Sakaue-Sawano & Atsushi Miyawaki
    • Organizer
      第11回日中合同組織細胞化学セミナー
    • Place of Presentation
      長野県松本市中央公民館(Mウイング文化センター)
    • Year and Date
      2014-09-28 – 2014-09-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 細胞社会のheterogeneityを視る技術2014

    • Author(s)
      阪上-沢野 朝子
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Observing heterogeneity in a cell society2014

    • Author(s)
      Asako Sakaue-Sawano & Atsushi Miyawaki
    • Organizer
      The 37th Naito Conference
    • Place of Presentation
      Hilton Niseko Village, Hokkaido, Japan
    • Year and Date
      2014-07-15 – 2014-07-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞動態を4次元で理解するための技術開発2014

    • Author(s)
      阪上―沢野 朝子 宮脇 敦史
    • Organizer
      日本顕微鏡学会第70回記念学術講演会
    • Place of Presentation
      幕張メッセ国際会議場
    • Year and Date
      2014-05-11 – 2014-05-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 細胞社会を4次元で理解するための技術開発2014

    • Author(s)
      Asako Sakaue-Sawano & Atsushi Miyawaki
    • Organizer
      日本顕微鏡学会 バイオメディカルニュ-マイクロスコ-プ分科会
    • Place of Presentation
      帝京大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Heterogeneity in a cell society2013

    • Author(s)
      Asako Sakaue-Sawano & Atsushi Miyawaki
    • Organizer
      RC, Aging, Biology of
    • Place of Presentation
      Lucca (Barga), Italy
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞動態を4次元で理解するための技術開発2013

    • Author(s)
      阪上―沢野 朝子 宮脇 敦史
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Observing heterogeneity in cancer society2013

    • Author(s)
      Asako Sakaue-Sawano & Atsushi Miyawaki
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] Laboratory for Cell Function Dynamics _ Fucci

    • URL

      http://cfds.brain.riken.jp/Fucci.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] Fucci

    • URL

      http://cfds.brain.riken.jp/Fucci.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi