Mechanism of assembly and disassembly of RNA granules responsible for mRNA transport and translational regulation
| Project/Area Number |
25440099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
SHIINA Nobuyuki 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 准教授 (30332175)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Kei 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (40553590)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | RNA granule / 翻訳制御 / リン酸化 / メチル化 / RNG105 / NF45 / NFAR2 / PRMT1 / ストレス / NFAR2/NF110 / PKR / NF110 |
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| Outline of Final Research Achievements |
The regulation of RNA granule assembly and disassembly is a structural basis of translational control. Here, we analyzed the mechanism of RNA granule assembly and disassembly regulated by NFAR2-NF45 complex and RNA granule disassembly regulated by PRMT1. NFAR2 enhanced the assembly of RNA granules, whereas NF45 promoted disassembly of RNA granules by binding to NFAR2 and inhibiting the ability of NFAR2 to assemble RNA granules. PRMT1 methylated RNG105, which reduced the ability of RNG105 to promote RNA granule assembly and resulted in the disassembly of RNA granules. These results provided novel mechanisms of assembly and disassembly of RNA granules.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Disrupted-in-schizophrenia 1 regulates transport of ITPR1 mRNA for synaptic plasticity.2015
Author(s)
Tsuboi D, Kuroda K, Tanaka M, Namba T, Iizuka Y, Taya S, Shinoda T, Hikita T, Muraoka S, Iizuka M, Nimura A, Mizoguchi A, Shiina N, Sokabe M, Okano H, Mikoshiba K, Kaibuchi K
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Journal Title
Nature Neuroscience
Volume: in press
Issue: 5
Pages: 698-707
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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