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Mechanism of assembly and disassembly of RNA granules responsible for mRNA transport and translational regulation

Research Project

Project/Area Number 25440099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

SHIINA Nobuyuki  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 准教授 (30332175)

Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Kei  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (40553590)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsRNA granule / 翻訳制御 / リン酸化 / メチル化 / RNG105 / NF45 / NFAR2 / PRMT1 / ストレス / NFAR2/NF110 / PKR / NF110
Outline of Final Research Achievements

The regulation of RNA granule assembly and disassembly is a structural basis of translational control. Here, we analyzed the mechanism of RNA granule assembly and disassembly regulated by NFAR2-NF45 complex and RNA granule disassembly regulated by PRMT1. NFAR2 enhanced the assembly of RNA granules, whereas NF45 promoted disassembly of RNA granules by binding to NFAR2 and inhibiting the ability of NFAR2 to assemble RNA granules. PRMT1 methylated RNG105, which reduced the ability of RNG105 to promote RNA granule assembly and resulted in the disassembly of RNA granules. These results provided novel mechanisms of assembly and disassembly of RNA granules.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Comprehensive behavioral analysis of RNG105 (Caprin1) heterozygous mice: Reduced social interaction and attenuated response to novelty.2016

    • Author(s)
      Ohashi R, Takao K, Miyakawa T, Shiina N
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 20775-20775

    • DOI

      10.1038/srep20775

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disrupted-in-schizophrenia 1 regulates transport of ITPR1 mRNA for synaptic plasticity.2015

    • Author(s)
      Tsuboi D, Kuroda K, Tanaka M, Namba T, Iizuka Y, Taya S, Shinoda T, Hikita T, Muraoka S, Iizuka M, Nimura A, Mizoguchi A, Shiina N, Sokabe M, Okano H, Mikoshiba K, Kaibuchi K
    • Journal Title

      Nature Neuroscience

      Volume: in press Issue: 5 Pages: 698-707

    • DOI

      10.1038/nn.3984

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] RNA granule assembly and disassembly modulated by nuclear factor associated with double-stranded RNA 2 and nuclear factor 452014

    • Author(s)
      Nobuyuki Shiina, Kei Nakayama
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 30 Pages: 21163-21180

    • DOI

      10.1074/jbc.m114.556365

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] RNG105ヘテロ欠損マウスの網羅的行動解析―社会的相互作用・新奇対象への興味の低下2015

    • Author(s)
      大橋りえ、高雄啓三、宮川剛、椎名伸之
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経RNA granuleのダイナミクスと高次脳機能・精神神経疾患2015

    • Author(s)
      椎名伸之
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 記憶形成に関与するRNG105が神経樹状突起へ輸送するmRNAの網羅的同定2015

    • Author(s)
      大橋りえ、重信秀治、椎名伸之
    • Organizer
      第38回神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA granule構成因子RNG105は記憶の成立に関与する2015

    • Author(s)
      中山啓、阿部学、山崎真弥、藤川顕寛、野田昌晴、二木啓、御子柴克彦、崎村建司、椎名伸之
    • Organizer
      第38回神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA granuleのダイナミクスと高次脳機能2015

    • Author(s)
      椎名伸之
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経樹状突起mRNA輸送・局所的翻訳と記憶形成2014

    • Author(s)
      椎名伸之
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 記憶形成に関与するRNG105が神経樹状突起へ輸送するmRNAの網羅的同定2014

    • Author(s)
      大橋りえ、重信秀治、椎名伸之
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA granule構成因子RNG105は記憶の成立に関与する2014

    • Author(s)
      中山啓、阿部学、山崎真弥、藤川顕寛、野田昌晴、二木啓、御子柴克彦、崎村建司、椎名伸之
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA granule集合・離散のNFAR2/NF110による制御機構2013

    • Author(s)
      椎名伸之、中山啓
    • Organizer
      第65回 日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち(愛知県)
    • Related Report
      2013 Research-status Report
  • [Presentation] RNA結合タンパク質RNG105コンディショナルノックアウトマウスにおける学習・記憶の解析2013

    • Author(s)
      中山啓、阿部学、山崎真弥、二木啓、御子柴克彦、崎村建司、椎名伸之
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Research-status Report
  • [Remarks] 基礎生物学研究所プレスリリース

    • URL

      http://www.nibb.ac.jp/press/2016/02/11.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 記憶、神経変性疾患、ストレス防御などに関与するRNA粒子の集合・離散の新たな仕組みを解明

    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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