Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Outline of Final Research Achievements |
The research goal is to understand the molecular mechanisms of the linkage that connects the embryonic axis specification and formation in the sea urchin. My previous work showed that the secondary axis specifier Nodal suppressed the primary axis formation. To investigate the detailed molecular mechanisms of this linkage, we blocked the function of Wnt7, as a ligand candidate for the canonical Wnt pathway. In Nodal morphants, the anterior neuroectoderm (animal plate: AP) is more restricted than that in normal embryos. However, when Wnt7 function is blocked simultaneously, the excess restriction is never happened. This result indicates that double suppression mechanism regulates the precise size control of AP region: canonical Wnt suppresses neuroectoderm and Nodal suppresses the canonical Wnt. This leads us to understand how the cell-fate is specified along with the orthogonal body axes during the early embryogenesis.
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