| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The cerebellum plays an important role for motor learning and classical fear conditioning. To understand the genes that control the generation of cerebellar granule cells and their axogenesis, I investigated two zebrafish mutants. The cranio facial development protein 1 gene controlled granule cell defferentiation by switching proliferation state to differentiation state in the precursors. The component of beaement-membrane, type IV collagens, regulated the axogenesis. I established a method for the classical fear conditioning paradigm by using zebrafish larva. The 20 days zebrafish larvae acquired the conditioned fear responses. Inhibition of granule-cell transmission impaired the recovery from conditioned responses. I found conditioning-dependent changes in granule cell encoding. There were two types of conditioning-associated neurons in the cerebellum. These results indicate that granule cells control the recovery from conditioned fear responses in zebrafish.
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