| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Skin pigmentation is achieved through an intercellular transfer of melanin pigments from melanocytes to surrounding keratinocytes. Our live imaging has visualized that the melanosomes are transferred via three pathways, membrane vesicles (small vesicles), filopodia and large vesicles. In this study, we attempted to understand molecular mechanisms and diversity of melanosome transfer. We first clarified that small GTPase Rho regulates membrane vesicles (small vesicles) by several different experiments. Second, we have identified keratinocyte-derived molecule that induce vesicle generation, especially larger vesicles. Finally, to understand conservation and diversity of intercellular transfer, we have examined extracellular vesicles from tumor cells during metastasis.
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