Evolutionary developmental analysis for the origin and migration of primordial germ cells

• Project/Area Number: 25440108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Institute of Physical and Chemical Research (2014-2016); Kobe University (2013)
• Principal Investigator: Kusakabe Rie 国立研究開発法人理化学研究所, 倉谷形態進化研究室, 研究員 (70373298)
• Research Collaborator: INOUE Kunio 神戸大学, 大学院理学研究科, 教授 (40252415)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖細胞 / 脊椎動物 / 神経堤細胞 / 細胞分化 / 筋肉 / ヤツメウナギ / 進化 / 遺伝子発現 / RNA / 生殖巣

Outline of Final Research Achievements

The cyclostome lampreys, a group of vertebrates that diverged early in evolution, retain various ancestral morphological features. In order to clarify the evolutionary pathway of origin and developmental behavior of undifferentiated precursor cells, including primordial germ cells, we conducted examination of expression patterns of developmental genes and histological observation. We also attempted the new experimental techniques for lineage tracing after introduction of artificial gene constructs. Comparison with insights from other vertebrate species implied the evolutionary relationships of the migratory undifferentiated precursor cells and the establishment of complex and diverse morphology of vertebrates.

Research Products

• [Journal Article] ヤツメウナギで解き明かす進化の謎 (2016)
  • Author(s): 日下部りえ
  • Journal Title: 実験医学 Volume: 34 Pages: 3086-3090
• [Journal Article] Developmental regulation and evolution of muscle-specific microRNA (2015)
  • Author(s): Rie Kusakabe, Kunio Inoue
  • Journal Title: Sem. Cell Dev. Biol. Volume: 47-48 Pages: 9-16
  • DOI: 10.1016/j.semcdb.2015.10.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] miR-124 is involved in post-transcriptional regulation of polypyrimidine tract binding protein 1 (PTBP1) during neural development in the medaka, Oryzias latipes. (2013)
  • Author(s): Yumiko Kato, Rie Kusakabe, Kunio Inoue and Shin Tochinai.
  • Journal Title: Zool. Sci. Volume: 30 Issue: 11 Pages: 891-900
  • DOI: 10.2108/zsj.30.891
  • NAID: 40019863773
  • Peer Reviewed
• [Journal Article] Body wall development in lamprey and a new perspective on the origin of vertebrate paired fins (2013)
  • Author(s): Tulenko, F. J., McCauley, D.W., Mackenzie, E.L., Mazan, S., Kuratani, S., Sugahara, F., Kusakabe, R., Burke, A.C.
  • Journal Title: PNAS Volume: 110(29) Issue: 29 Pages: 11899-11904
  • DOI: 10.1073/pnas.1304210110
  • Peer Reviewed
• [Journal Article] Developmental expression and evolution of muscle-specific microRNA s conserved in vertebrates. (2013)
  • Author(s): Saori Tani, Shigehiro Kuralcu, Hiroshi Sakamoto, Kunio Inoue and Rie Kusakabe.
  • Journal Title: Evolution & Development Volume: 15 Issue: 4 Pages: 293-304
  • DOI: 10.1111/ede.12039
  • Peer Reviewed
• [Presentation] Evolution and development of skeletal musculature in early vertebrates: with special reference to the head/trunk (2016)
  • Author(s): Rie Kusakabe and Shigeru Kuratani
  • Organizer: International Symposium: Morphological Evolution of Vertebrates: The Joint meeting of The 22nd International Congress of Zoology amd the 87th meeting of Zoological Society of Japan
  • Place of Presentation: 沖縄科学技術大学院大学（沖縄県国頭郡恩納村）
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] Molecular basis for divergence and elaboration of skeletal muscles (2016)
  • Author(s): Rie Kusakabe
  • Organizer: 第4回若手による骨格筋細胞研究会 The 4th Society of Skeletal Muscle Cells
  • Place of Presentation: ウインクあいち（愛知県名古屋市）
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Development and evolution of mesodermal components of early vertebrates (2016)
  • Author(s): Rie Kusakabe and Shigeru Kuratani
  • Organizer: Society for Developmental Biology 75th Annual Meeting-International Society of Differentiation 19th International Conference
  • Place of Presentation: 米国マサチューセッツ州ボストン
  • Year and Date: 2016-08-04
  • Int'l Joint Research
• [Presentation] Divergence and elaboration of skeletal musculature in early vertebrates (2016)
  • Author(s): Rie Kusakabe and Shigeru Kuratani
  • Organizer: The 11th International Congress of Vertebrate Morphology
  • Place of Presentation: 米国ワシントンDC
  • Year and Date: 2016-07-02
  • Int'l Joint Research
• [Presentation] Evolution and development of muscles in the head/trunk interface of vertebrates (2016)
  • Author(s): Rie Kusakabe and Shigeru Kuratani
  • Organizer: Frontier of Myogenesis meeting: Molecular Mechanisms Modulating Skeletal Muscle Development and Homeostasis in Health and Disease
  • Place of Presentation: 米国カリフォルニア州アシロマー
  • Year and Date: 2016-06-08
  • Int'l Joint Research
• [Presentation] 円口類ヤツメウナギを用いた進化発生学 (2015)
  • Author(s): 日下部りえ
  • Organizer: ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
  • Place of Presentation: 基礎生物学研究所（岡崎市）
  • Year and Date: 2015-08-18
  • Invited
• [Presentation] Development and evolution of mesodermal components of the lamprey (2015)
  • Author(s): Rie Kusakabe
  • Organizer: Inaugral Meeting of the Pan-American Society for Evolutionary Developmental Biology
  • Place of Presentation: University of California at Berkeley
  • Year and Date: 2015-08-06
  • Int'l Joint Research
• [Presentation] 軸下骨格筋の多様性と発生メカニズム (2014)
  • Author(s): 日下部りえ
  • Organizer: 第85回日本動物学会大会
  • Place of Presentation: 東北大学
  • Year and Date: 2014-09-10 – 2014-09-13
• [Presentation] Evolution of regulatory mechanisms in hypaxial muscle development. (2013)
  • Author(s): Rie Kusakabe, Saori Tani, Richard M. Harland, Kunio Inoue, Shigeru Kuratani.
  • Organizer: 10th International Congress of Vertebrate Morphology
  • Place of Presentation: スペイン、バルセロナ
• [Presentation] Developmental evolution of the hypaxial muscles of vertebrates (2013)
  • Author(s): 17. Rie Kusakabe, Saori Tani, Richard M. Harland, Shigeru Kuratani and Kunio Inoue.
  • Organizer: 46th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 島根県松江市