Functional Analysis of grimp protein that expresses in regeneration stem cells in Enchytraeus japonensis
| Project/Area Number |
25440116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOCHINAI Shin 北海道大学, 理学研究科, 教授 (20111148)
MIKAMI Toshikazu 日本大学, 歯学部, 助教 (80434075)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 再生幹細胞 / 幹細胞増殖 / タンパク質機能解析 / ヤマトヒメミミズ / 無性生殖 / 細胞分化 / 細胞接着 / 免疫組織染色 / 遺伝子発現 / 抗体作成 |
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| Outline of Final Research Achievements |
grimp gene plays an important role in early stage of regeneration in Enchytraeus japonensis. grimp mRNA is detected transiently from 6 to 12 h post amputation only in mesodermal stem cells called neoblasts and mesodermal lineage cells. In order to clarify the function and cellular localization of grimp protein, we took approaches for the production, purification and characterization of recombinant protein, and made polyclonal antibodies. By immunohistochemistry, we found that grimp protein is expressed in the mesodermal cells in regeneration bud from 6-24 h post amputation. The target protein of the antibodies was detected by the western blotting at around predicted size.
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Report
(5 results)
Research Products
(52 results)
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[Journal Article] Short-term feeding at the wrong time is sufficient to desynchronize peripheral clocks and induce obesity with hyperphagia, physical inactivity and metabolic disorders in mice2016
Author(s)
Yasumoto Y, Hashimoto C, Nakao R, Yamazaki H, Hiroyama H, Nemoto T, Yamamoto S, Sakurai M, Oike H, Wada N, Yoshida-Noro C, Oishi K
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Journal Title
Metabolism
Volume: 65
Issue: 5
Pages: 714-727
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] DFAT細胞再生移植治療のための細胞キャリアの検討2017
Author(s)
野呂知加子, 三浦 大輝, 風間 智彦, 萩倉 一博, 松本 太郎
Organizer
日本大学学長特別研究「成熟細胞脱分化による組織再生メカニズムの解明と脱分化培養技術を用いた細胞治療研究」平成28年度 研究成果報告会
Place of Presentation
日本大学医学部 東京
Year and Date
2017-03-18
Related Report
Invited
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[Presentation] DFAT細胞による移植治療のための細胞キャリアの開発2017
Author(s)
三浦大輝, 風間智彦, 萩倉一博, 松本太郎, 野呂知加子
Organizer
文部科学省私立大学戦略的研究基盤形成支援事業「脱分化脂肪細胞を用いた細胞治療の臨床応用に向けた橋渡し研究」平成28年度 研究成果公開シンポジウム
Place of Presentation
日本大学医学部 東京
Year and Date
2017-03-11
Related Report
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[Presentation] ナミヒメハナカメムシの波長選好性2015
Author(s)
荻野拓海, 上原拓也, 山口照美, 野呂知加子, 前田太郎, 霜田政美
Organizer
第59回日本応用動物昆虫学会大会
Place of Presentation
山形大学小白川キャンパス 山形
Year and Date
2015-03-27
Related Report
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