Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Outline of Final Research Achievements |
The gene expression changes in Xenopus laevis cells by transfection with reprogramming factors were investigated. The correlation coefficients using all probes indicated that the gene expression profiles changed sequentially in 24 to 72 hours after transfection. Pluripotent markers, Oct3/4 orthologue oct-91 (pou5f3.1) and Cripto/TDGF1 orthologue FRL-1 (tdgf1.3), showed increased expression. ventx2.1-b (Xbr-1b) and ventx3.2 (Xvex-1) also showed upregulation of the expression, among vent/ventx family genes that are candidates of Nanog alternative.
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