Study of reprogramming in Xenopus cells that do not have Nanog gene

• Project/Area Number: 25440120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Onuma Yasuko 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (90431824)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI Mahito 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (10172355) ; TSUJI Shingo 国立大学法人東京大学, 駒場オープンラボラトリー, 特任助教 (80431823)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 幹細胞 / ツメガエル / リプログラミング / 多能性

Outline of Final Research Achievements

The gene expression changes in Xenopus laevis cells by transfection with reprogramming factors were investigated. The correlation coefficients using all probes indicated that the gene expression profiles changed sequentially in 24 to 72 hours after transfection. Pluripotent markers, Oct3/4 orthologue oct-91 (pou5f3.1) and Cripto/TDGF1 orthologue FRL-1 (tdgf1.3), showed increased expression. ventx2.1-b (Xbr-1b) and ventx3.2 (Xvex-1) also showed upregulation of the expression, among vent／ventx family genes that are candidates of Nanog alternative.