Elucidation of physiological roles of translational regulation mediated by upstream open reading frame-encoded peptides in plants
| Project/Area Number |
25440122
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
ONOUCHI Hitoshi 北海道大学, (連合)農学研究科(研究院), 准教授 (50322839)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | uORF / 翻訳制御 / リボソーム / 新生ペプチド / シロイヌナズナ / ポプラ |
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| Outline of Final Research Achievements |
Upstream open reading frames (uORFs) are small ORFs present in the 5′ untranslated regions of many eukaryotic mRNAs. Some uORFs encode regulatory peptides that repress translation of the main ORF. Such translational regulation mediated by a uORF-encoded peptide was considered to be an exceptional regulatory mechanism. However, we recently identified a variety of genes whose expression is regulated by a uORF-encoded peptide, suggesting that uORF peptide-mediated translational regulation plays a wider physiological role than previously thought. In this study, we investigated physiological roles of the regulatory uORF peptides that we identified, and found a uORF involved in developmental regulation and a uORF that specifically represses main ORF expression in actively proliferating cells. Furthermore, this study revealed a novel mechanism of translational regulation, which involves two uORF-encoded peptides that have opposite regulatory roles.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Identification of novel Arabidopsis thaliana upstream open reading frames that control expression of the main coding sequences in a peptide sequence-dependent manner.2015
Author(s)
Ebina I, Takemoto-Tsutsumi M, Watanabe S, Koyama H, Endo Y, Kimata K, Igarashi T, Murakami K, Kudo R, Ohsumi A, Noh AL, Takahashi H, Naito S, Onouchi H
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Journal Title
Nucleic Acids Research
Volume: 43
Issue: 3
Pages: 1562-1576
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A U-system approach for predicting metabolic behaviors and responses based on an alleged metabolic reaction network.2014
Author(s)
Sriyudthsak K, Sawada Y, Chiba Y, Yamashita Y, Kanaya S, Onouchi H, Fujiwara T, Naito S, Voit EO, Shiraishi F, Hirai MY
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Journal Title
BMC Systems Biology
Volume: 8
Issue: S5
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] SS-mPMG and SS-GA : tools for finding pathways and dynamic simulation of metabolic networks.2013
Author(s)
Tetsuo Katsuragi, Naoaki Ono, Keiichi Yasumoto, Masami Y. Hirai, Kansuporn Sriyudthsak, Yuji Sawada, Yui Yamashita, Yukako Chiba, Hitoshi Onouchi, Toru Fujiwara, Satoshi Naito
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Journal Title
Plant and Cell Physiology
Volume: 54
Issue: 5
Pages: 728-739
DOI
Related Report
Peer Reviewed
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