| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Upstream open reading frames (uORFs) are small ORFs present in the 5′ untranslated regions of many eukaryotic mRNAs. Some uORFs encode regulatory peptides that repress translation of the main ORF. Such translational regulation mediated by a uORF-encoded peptide was considered to be an exceptional regulatory mechanism. However, we recently identified a variety of genes whose expression is regulated by a uORF-encoded peptide, suggesting that uORF peptide-mediated translational regulation plays a wider physiological role than previously thought. In this study, we investigated physiological roles of the regulatory uORF peptides that we identified, and found a uORF involved in developmental regulation and a uORF that specifically represses main ORF expression in actively proliferating cells. Furthermore, this study revealed a novel mechanism of translational regulation, which involves two uORF-encoded peptides that have opposite regulatory roles.
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