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Identification of factors essential for the extracellular trafficking of cell wall polysaccharides

Research Project

Project/Area Number 25440124
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTohoku University

Principal Investigator

YOKOYAMA Ryusuke  東北大学, 生命科学研究科, 講師 (90302083)

Co-Investigator(Renkei-kenkyūsha) NISHITANI KAZUHIKO  東北大学, 大学院生命科学研究科, 教授 (60164555)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords植物細胞壁 / 細胞壁多糖 / 細胞間輸送 / イネ / キシログルカン / マイクロダイセクション / 細胞壁 / プロテオーム
Outline of Final Research Achievements

Plant cell wall plays several critical roles in determining the cell shape and functions, thereby having key roles in regulating plant growth and differentiation. The cell wall is a complex and dynamic structure that is a highly organized composite of many polysaccharides and proteins. The assembly and remodeling of the cell wall structure is involved in dynamic enzymes and substrates trafficking to the proper point in extracellular space. Various types of regulatory factors are supposed to contribute to the proper extracellular trafficking of the cell wall enzymes and substrates. To date however, the factors involved in regulation of the extracellular trafficking are almost unknown. The present study toward the characterization of trafficking regulatory factors offers an improved model for understanding the trafficking system and cell wall construction.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (32 results)

All 2016 2015 2014 2013 Other

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (18 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Possible pathways linking ploidy level to cell elongation and cuticular function in hypocotyls of dark-grown Arabidopsis seedlings2016

    • Author(s)
      Narukawa, H., Yokoyama, R., Nishitani, K.
    • Journal Title

      Plant Signal. Behav.

      Volume: 11 Issue: 2 Pages: e1118597-e1118597

    • DOI

      10.1080/15592324.2015.1118597

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein Ligand-tethered Synthetic Calcium Indicator for Localization Control and Spatiotemporal Calcium Imaging in Plant Cells2016

    • Author(s)
      Y. Takaoka, M. Shigenaga, Y. Nukadzuka, M. Imai, Y. Ishimaru, K. Saito, R. Yokoyama, K. Nishitani, M. Ueda
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 26 Issue: 1 Pages: 9-14

    • DOI

      10.1016/j.bmcl.2015.11.055

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Immunogold labeling analysis of cell wall polysaccharides with special reference to (1;3, 1;4)-β-D-glucan in rice cell walls2016

    • Author(s)
      Ryusuke Yokoyama, 他
    • Journal Title

      Bio-protocol

      Volume: 6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stimulation of cell elongation by tetraploidy in hypocotyls of dark-grown Arabidopsis seedlings.2015

    • Author(s)
      Narukawa, H., Yokoyama, R., Komaki, S., Sugimoto, K., Nishitani, K.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 8 Pages: e0134547-e0134547

    • DOI

      10.1371/journal.pone.0134547

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histochemical analysis of silica body staining in rice leaf blades2015

    • Author(s)
      Ryusuke Yokoyama 他
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The matrix polysaccharide (1;3,1;4)-β-D-glucan is involved in silicon-dependent strengthening of rice cell wall2015

    • Author(s)
      Natsumi Kido, Ryusuke Yokoyama, 他
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 2 Pages: 268-276

    • DOI

      10.1093/pcp/pcu162

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 植物細胞壁:高次構造の構築と再編2015

    • Author(s)
      横山隆亮, 他
    • Journal Title

      化学と生物

      Volume: 53 Pages: 107-114

    • NAID

      130005120438

    • Related Report
      2014 Research-status Report
  • [Journal Article] The biosynthesis and function of polysaccharide components of the plant cell wall2014

    • Author(s)
      Yokoyama, R., Shinohara, N., Asaoka, R., Narukawa, H., Nishitani, K.
    • Journal Title

      Plant Cell Wall Patterning and Cell Shape

      Volume: 48 Pages: 1-34

    • DOI

      10.1002/9781118647363.ch1

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] XTH20 and XTH19 regulated by ANAC071 under auxin flow are involved in cell proliferation in incised Arabidopsis inflorescence stems2014

    • Author(s)
      Pitaksaringkarn, W., Matsuoka, K., Asahina, M., Miura, K., Sage-Ono, K., Ono, M., Yokoyama, R., Nishitani, K., Ishii, T., Iwai, H., Satoh, S.
    • Journal Title

      Plant Journal

      Volume: 80 Issue: 4 Pages: 604

    • DOI

      10.1111/tpj.12654

    • NAID

      120007136554

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Function of xyloglucan endotransglucosylase/hydrolases in rice. Function of xyloglucan endotransglucosylase/hydrolases in rice.2013

    • Author(s)
      Hara,Y., et al.
    • Journal Title

      Ann. Bot

      Volume: 1 Issue: 6 Pages: 1-1

    • DOI

      10.1093/aob/mct292

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Transglycosylation on cellulosic polysaccharides by member of the arabidopsis xyloglucan-endotransglucosylase/hydrolase family2016

    • Author(s)
      Naoki Shinohara, 他
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Imaging analysis of cell wall regeneration in mesophyll protoplast derived from Arabidopsis thaliana2016

    • Author(s)
      Hiroaki Kuki, 他
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] プロトプラスト細胞壁再生系とイメージング解析を用いたセルロースネットワーク構築機構の解析2015

    • Author(s)
      九鬼寛明、他
    • Organizer
      東北植物学会第5回大会
    • Place of Presentation
      福島大学(福島)
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞壁構築におけるβ-(1→3), (1→4)-グルカンの機能解明2015

    • Author(s)
      竹内美和、他
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜交通過程におけるシロイヌナズナのペクチンメチルエステラーゼPME35の機能制御について2015

    • Author(s)
      小浜大山、他
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] アメリカネナシカズラの寄生メカニズムの解明2015

    • Author(s)
      柴田航希、他
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロイヌナズナの4倍体を用いた細胞サイズ決定メカニズムの解析2015

    • Author(s)
      鳴川秀樹、他
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Temporal and spatial regulation of cell wall regeneration on arabidopsis mesophyll protoplasts2015

    • Author(s)
      Ryusuke Yokoyama 他
    • Organizer
      The 26th International Conference on Arabidopsis Research
    • Place of Presentation
      Paris, France
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物プロトプラストの細胞壁再生の時間的・空間的制御機構2015

    • Author(s)
      横山隆亮,他
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] エンド型キシログルカン転移素/加水分解酵素ファミリーの系統的基部にある PpXTH32 の酵素機能解析2014

    • Author(s)
      藤田康平,他
    • Organizer
      東北植物学会第4回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2014-12-13 – 2014-12-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 葉肉細胞プロトプラストの細胞壁再生系の開発とイメージング解析を利用した細胞壁構築過程の解析2014

    • Author(s)
      九鬼寛明,他
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナ 4 倍体を用いた細胞サイズ決定メカニズムの解析2014

    • Author(s)
      鳴川秀樹,他
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Role of (1,3;1,4)-beta-D-glucan in rice growth and development2014

    • Author(s)
      Ryusuke Yokoyama、他
    • Organizer
      The 5th International Conference on Plant Cell Wall Biology
    • Place of Presentation
      Queensland, Australia
    • Year and Date
      2014-07-27 – 2014-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] レーザーマイクロダイセクション法によるネナシカズラ寄生根の組織特異的な オミクス解析を目指して2014

    • Author(s)
      横山隆亮、他
    • Organizer
      ワークショップ「茎寄生植物ネナシカズラ研究事始め」
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 葉肉細胞プロトプラストを用いた細胞壁再生過程に関与するタンパク質群の解析2014

    • Author(s)
      小山大山、九鬼寛明、横山隆亮、西谷和彦、他
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] The matrix polysaccharide (1;3,1;4)-beta-D-glucan is involved in silicon-dependent strengthening of rice cell wall2014

    • Author(s)
      金子麻里、横山隆亮、西谷和彦、他
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Role of polysaccharides in rice growth and development2013

    • Author(s)
      Ryusuke Yokoyama、他
    • Organizer
      XIII Cell Wall Meeting
    • Place of Presentation
      Nantes, France
    • Related Report
      2013 Research-status Report
  • [Presentation] バイオマス利用のためのイネの細胞壁研究2013

    • Author(s)
      横山隆亮
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Atlas of Plant Cell Structure2014

    • Author(s)
      Noguchi Tetsuko, 他
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Remarks] 東北大学大学院生命科学研究科植物細胞壁機能分野

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東北大学大学院生命科学研究科植物細胞壁機能分野

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2014 Research-status Report
  • [Remarks] 東北大学大学院生命科学研究科植物細胞壁機能分野

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2023-03-16  

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