Self-organization of ROP GTPases and cortical microtubules in plant cell morphogenesis

• Project/Area Number: 25440128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute of Genetics (2014-2015); The University of Tokyo (2013)
• Principal Investigator: Oda Yoshihisa 国立遺伝学研究所, 新分野創造センター, 准教授 (30583257)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 表層微小管 / 木部道管分化 / 二次細胞壁 / 低分子量GTPase / ROP GTPase / Kinesin-13 / 微小管 / キネシン / 細胞壁

Outline of Final Research Achievements

Plant development largely depends on well-organized cell walls which determines the shape of each plant cell. I focused on how plant cortical microtubules and ROP GTPases spatially interact each other to determine the deposition pattern of cell walls in xylem vessels cells. I revealed that ROP GTPases mutually inhibit cortical microtubules through MIDD1-Kinesin-13A complex and possibly through BDR1, a novel protein of unknown function. I also found that VETH1 may mediate membrane trafficking of cell wall components along cortical microtubules. These findings may contribute to our further understanding of plant and plant cell morphogenesis.

Research Products

• [Journal Article] Cortical microtubule rearrangements and cell wall patterning (2015)
  • Author(s): Oda, Y.
  • Journal Title: Front Plant Sci Volume: 6 Pages: 236-239
  • DOI: 10.3389/fpls.2015.00236
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel Coiled-Coil Proteins Regulate Exocyst Association with Cortical Microtubules in Xylem Cells via the Conserved Oligomeric Golgi-Complex 2 Protein (2015)
  • Author(s): Oda, Y., Iida, Y., Nagashima, Y., Sugiyama, Y., Fukuda, H.
  • Journal Title: Plant Cell Physiol Volume: 56 Issue: 2 Pages: 277-286
  • DOI: 10.1093/pcp/pcu197
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A bHLH Complex Activates Vascular Cell Division via Cytokinin Action in Root Apical Meristem (2014)
  • Author(s): Kyoko Ohashi-Ito, Maria Saegusa, Kuninori Iwamoto, Yoshihisa Oda, Hirofumi Katayama, Mikiko Kojima, Hitoshi Sakakibara and Hiroo Fukuda
  • Journal Title: Current Biology Volume: 24 Issue: 17 Pages: 2053-2058
  • DOI: 10.1016/j.cub.2014.07.050
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Emerging roles of small GTPases in secondary cell wall development (2014)
  • Author(s): Oda, Y., Fukuda, H.
  • Journal Title: Front Plant Sci Volume: 5 Pages: 428-432
  • DOI: 10.3389/fpls.2014.00428
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The dynamic interplay of plasma membrane domains and cortical microtubules in secondary cell wall patterning. (2013)
  • Author(s): Oda, Y., and Fukuda, H.
  • Journal Title: Frontiers in Plant Sci. Volume: 4 Pages: 1-6
  • DOI: 10.3389/fpls.2013.00511
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rho of Plant GTPase signaling regulates the behavior of Arabidopsis Kinesin-13A to establish secondary cell wall patterns (2013)
  • Author(s): Oda Y, Fukuda H
  • Journal Title: Plant Cell Volume: 25 Issue: 11 Pages: 4439-4450
  • DOI: 10.1105/tpc.113.117853
  • Peer Reviewed
• [Journal Article] Spatial organization of xylem cell walls by ROP GTPases and microtubule-associated proteins. (2013)
  • Author(s): Oda, Y., and Fukuda, H.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Issue: 6 Pages: 743-748
  • DOI: 10.1016/j.pbi.2013.10.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Visualization and analysis of molecular components of secondary cell wall patterning (2013)
  • Author(s): Oda Y, Fukuda H
  • Journal Title: Plant Morphology Volume: 25 Pages: 45-50
  • NAID: 130004691672
• [Journal Article] Molecular basis of secondary cell wall patterning (2013)
  • Author(s): Oda Y, Fukuda H
  • Journal Title: Cell Commu Volume: 20 Pages: 125-129
  • NAID: 40019799814
• [Journal Article] 二次細胞壁パターンの制御機構 (2013)
  • Author(s): 小田祥久、福田裕穂
  • Journal Title: 化学と生物 Volume: 51 Pages: 795-801
  • NAID: 130004707388
  • Peer Reviewed
• [Presentation] 二次細胞壁パターンの獲得における新規微小管付随タンパク質の機能解析 (2016)
  • Author(s): 杉山友希、若崎眞由美、佐藤繭子、豊岡公徳、福田裕穂、小田祥久
  • Organizer: 第５７回日本植物生理学会年会
  • Place of Presentation: 岩手大学、盛岡、岩手
  • Year and Date: 2016-03-19
• [Presentation] 道管において二次細胞壁パターンを協調的に制御するROP GTPaseシグナルの解析 (2016)
  • Author(s): 長島慶宜、福田裕穂、小田祥久
  • Organizer: 第５７回日本植物生理学会年会
  • Place of Presentation: 岩手大学、盛岡、岩手
  • Year and Date: 2016-03-18
• [Presentation] Two-dimensional microtubule organization in plant xylem cells (2016)
  • Author(s): Oda, Y.
  • Organizer: NIG International Symposium 2016 "Quantitative Biology: force, information and dynamics: X factors shaping living systems"
  • Place of Presentation: National Institute of Genetics, Mishima, Japan
  • Year and Date: 2016-01-12
  • Int'l Joint Research / Invited
• [Presentation] Cortical microtubule patterning in xylem cells (2015)
  • Author(s): Oda, Y.
  • Organizer: Biochemistry and Moleclar Biology 2015 (BMB2015)
  • Place of Presentation: Kobe Port Island, Kobe, Japan
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] Molecular basis of plant cell wall patterning: a Rho GTPase-driven symmetry breaking in the two-dimensional microtubule organization (2015)
  • Author(s): Oda, Y.
  • Organizer: iCeMS International Symposium Hierarchical Dynamics in Soft Materials and Biological Matter
  • Place of Presentation: Kyoto University, Kyoto, Japan
  • Year and Date: 2015-09-26
  • Int'l Joint Research / Invited
• [Presentation] Spontaneous pattern formation of Rho GTPase in xylem vessel cells (2015)
  • Author(s): Nagashima, Y., Fukuda, H., Oda, Y.
  • Organizer: iCeMS International Symposium Hierarchical Dynamics in Soft Materials and Biological Matter
  • Place of Presentation: Kyoto University, Kyoto, Japan
  • Year and Date: 2015-09-24
• [Presentation] 表層微小管の動態制御に基いた二次細胞壁の空間構築 (2015)
  • Author(s): 杉山友希、福田裕穂、小田祥久
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ、新潟
  • Year and Date: 2015-09-08
• [Presentation] 道管において壁孔の空間配置を制御するROP GTPaseシグナルの解析 (2015)
  • Author(s): 長島慶宜、福田裕穂、小田祥久
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ、新潟
  • Year and Date: 2015-09-08
• [Presentation] 木部道管への片道切符：細胞壁の不可逆的な機能転換 (2015)
  • Author(s): 小田祥久、長島慶宜、杉山友希、佐々木武馬、福田裕穂
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ、新潟
  • Year and Date: 2015-09-06
• [Presentation] Secondary cell wall patterning in metaxylem vessels (2015)
  • Author(s): Oda, Y.
  • Organizer: The 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] Secondary cell wall patterning in metaxylem vessels (2015)
  • Author(s): Oda, Y., Nagashima, Y., Sugiyama, Y., Fukuda, H.
  • Organizer: ront Lines of Plant Cell Wall Research
  • Place of Presentation: 東大寺総合文化センター、奈良市
  • Year and Date: 2015-03-19 – 2015-03-20
  • Invited
• [Presentation] 二次細胞壁パターン形成の理解に向けた新規微小管付随タンパク質の解析 (2015)
  • Author(s): 杉山友希、福田裕穂、小田祥久
  • Organizer: 第５６回日本植物生理学会年会
  • Place of Presentation: 東京・東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 道管の二次細胞壁パターンを制御する新規ROP エフェクターの解析 (2015)
  • Author(s): 長島慶宜, 福田裕穂, 小田祥久
  • Organizer: 第５６回日本植物生理学会年会
  • Place of Presentation: 東京・東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Novel Coiled-Coil Proteins Regulate Exocyst Association with Cortical Microtubules in Xylem Cells (2015)
  • Author(s): Oda, Y., Iida, Y., Nagashima, Y., Sugiyama, Y., Fukuda, H.
  • Organizer: 第５６回日本植物生理学会年会
  • Place of Presentation: 東京・東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] The molecular basis of secondary cell wall patterns: Dynamic interplay of Rho GTPase and cortical cytoskeleton. (2014)
  • Author(s): Oda, Y., Nagashima, Y., Sugiyama, Y., Fukuda, H.
  • Organizer: 38th Naito Conference
  • Place of Presentation: 札幌・シャトレーゼ ガトーキングダムサッポロ
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] 二次細胞壁のパターン構築を制御する新規微小管付随タンパク質の同定 (2014)
  • Author(s): 杉山友希、福田裕穂、小田祥久
  • Organizer: 日本植物学会年会第７８回大会
  • Place of Presentation: 神奈川・川崎市・明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 道管の二次細胞壁パターンを制御する新規ROPシグナル因子の解析 (2014)
  • Author(s): 長島慶宜、福田裕穂、小田祥久
  • Organizer: 日本植物学会年会第７８回大会
  • Place of Presentation: 神奈川・川崎市・明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Live cell imaging of xylem differentiation: dissecting the molecular basis of secondary cell wall patterning. (2014)
  • Author(s): Oda, Y.
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium 2014 “Plant Live-Cell Imaging and Microdevices”
  • Place of Presentation: 名古屋市・名古屋大学東山キャンパス
  • Year and Date: 2014-09-09 – 2014-09-10
  • Invited
• [Presentation] Self-organization of cell wall patterns through the Rho GTPase-cortical microtubule interplay. (2014)
  • Author(s): Oda, Y.
  • Organizer: ESF/EMBO Conference (Cell polarity and membrane trafficking)
  • Place of Presentation: Polonia Castle (Dom Polonii), Pultusk, Poland
  • Year and Date: 2014-05-10 – 2014-05-15
  • Invited
• [Presentation] 細胞壁パターンを導く空間シグナルの動態 (2014)
  • Author(s): 小田祥久, 長島慶宜, 杉山友希, 福田裕穂
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Invited
• [Presentation] 二次細胞壁形成時に機能する新規微小管付随タンパク質の同定 (2014)
  • Author(s): 杉山友希, 小田祥久, 福田裕穂
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] 道管分化における新規壁孔局在因子の解析 (2014)
  • Author(s): 長島慶宜, 小田祥久, 福田裕穂
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] WOX4導入シロイヌナズナ培養細胞系を用いたWOX4下流因子の探索 (2013)
  • Author(s): 玉置貴之、小田祥久、福田裕穂
  • Organizer: 日本植物学会年会第７７回大会
  • Place of Presentation: 北海道大学
• [Presentation] Secondary cell wall patterning by Rop- and microtubule-driven symmetry breaking (2013)
  • Author(s): 小田祥久, 福田裕穂
  • Organizer: 日本植物学会年会第７７回大会
  • Place of Presentation: 北海道大学
  • Invited
• [Presentation] Initiation of Cell Wall Pattern by a Rho GTPase- and Microtubule-Driven Symmetry Breaking (2013)
  • Author(s): Yoshihisa Oda, Hiroo Fukuda
  • Organizer: The 46th annual meeting for the Japanese society of developmental biologists
  • Place of Presentation: 島根県松江市くにびきメッセ
  • Invited
• [Book] The Plant Cell Wall Pattern and Cell Shape. 1st ed, by Hiroo Fukuda, Chapter 7 (2014)
  • Author(s): Oda, Y., Fukuda, H.
  • Publisher: Wiley
• [Book] The Plant Cell Wall and Cell Shape, Chapter7 (2014)
  • Author(s): Yoshihisa Oda, Hiroo Fukuda
  • Total Pages: 37
  • Publisher: Wily