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Elucidation of nitrogen signaling in plants

Research Project

Project/Area Number 25440129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Imamura Sousuke  東京工業大学, 資源化学研究所, 准教授 (70548122)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords窒素 / 窒素代謝 / 紅藻
Outline of Final Research Achievements

Nitrogen is the component of several biomolecules, such as amino acids and nucleic acids, and thus key nutrient for plant cell growth. Although regulation of nitrogen including perception of nitrogen status and its signal transduction are well documented in fungi and mammals, the related information in plant cells are very poor. Previous our study revealed that MYB1, an R2R3-type MYB transcription factor, is involved in nitrogen depletion responsive expression of nitrogen assimilation genes in the unicellular red alga, Cyanidioschyzon merolae. In this study, I tried to identify and examine an upstream regulator for nitrogen depletion responses, such as MYB1-mediated transcription. As the result on the research, I demonstrated that TOR (target of rapamycin) functions in nitrogen responses including MYB1-mediated transcription as an upstream regulator of nitrogen regulation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (29 results)

All 2016 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (21 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Journal Article] TOR (target of rapamycin) is a key regulator of triacylglycerol accumulation in microalgae.2016

    • Author(s)
      Sousuke Imamura, Yasuko Kawase, Ikki Kobayashi, Mie Shimojima, Hiroyuki Ohta, and Kan Tanaka
    • Journal Title

      Plant Signal. Behav.

      Volume: in press Issue: 3 Pages: e1149285-e1149285

    • DOI

      10.1080/15592324.2016.1149285

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a <i>URA5.3</i> deletion strain of the unicellular red alga <i>Cyanidioschyzon merolae</i>: A backgroundless host strain for transformation experiments2015

    • Author(s)
      Keiko Taki, Toshiyuki Sone, Yuki Kobayashi, Satoru Watanabe, Sousuke Imamura, and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 61 Issue: 5 Pages: 211-214

    • DOI

      10.2323/jgam.61.211

    • NAID

      130005110096

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae2015

    • Author(s)
      Imamura S, Kawase Y, Kobayashi I, Sone T, Era A, Miyagishima SY, Shimojima M, Ohta H, Tanaka K.
    • Journal Title

      Plant Mol. Biol.

      Volume: 89 Issue: 3 Pages: 309-318

    • DOI

      10.1007/s11103-015-0370-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae2015

    • Author(s)
      Yu Kanesaki, Sousuke Imamura, Motomichi Matsuzaki, and Kan Tanaka
    • Journal Title

      FEBS Lett.

      Volume: 589 Issue: 11 Pages: 1219-1224

    • DOI

      10.1016/j.febslet.2015.04.009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The nuclear-encoded sigma factor SIG4 directly activates transcription of chloroplast psbA and ycf17 genes in the unicellular red alga Cyanidioschyzon merolae2015

    • Author(s)
      Gaku Fujii, Sousuke Imamura, Atsuko Era, Shin-ya Miyagishima, Mitsumasa Hanaoka, and Kan Tanaka
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 印刷中 Issue: 10

    • DOI

      10.1093/femsle/fnv063

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stable Expression of a GFP-reporter Gene in the Red AlgaCyanidioschyzon merolae2014

    • Author(s)
      Satoru Watanabe, Jun Sato, Sousuke Imamura, Mio Ohnuma, Yusaku Ohba, Taku Chibazakura, Kan Tanaka and Hirofumi Yoshikawa
    • Journal Title

      Biosci..Biotechnol. Biochem.

      Volume: 78 Issue: 1 Pages: 175-177

    • DOI

      10.1080/09168451.2014.877823

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of budding yeast FKBP12 confers rapamycin susceptibility to the unicellular red alga Cyanidioschyzon merolae.2013

    • Author(s)
      Imamura S, Ishiwata A, Watanabe S, Yoshikawa H, Tanaka K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 439 Issue: 2 Pages: 264-269

    • DOI

      10.1016/j.bbrc.2013.08.045

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear-encoded chloroplast RNA polymerase sigma factor SIG2 activates chloroplast-encoded phycobilisome genes in a red alga, Cyanidioschyzon merolae.2013

    • Author(s)
      Fujii G, Imamura S, Hanaoka M, Tanaka K.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 20 Pages: 3354-3359

    • DOI

      10.1016/j.febslet.2013.08.031

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおける窒素欠乏応答低分子量GタンパクCmRAB5の機能解析2016

    • Author(s)
      竹村時空、小林勇気、瀧景子、今村壮輔、田中寛
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻類において暗誘導されるR1型MYB転写因子MYB2の機能解析2016

    • Author(s)
      河瀬泰子、今村壮輔、田中寛
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeのトリアシルグリセロール-3-リン酸アシルトランスフェラーゼの機能解析(2016

    • Author(s)
      平澤英里、田中 寛、今村壮輔
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微細藻類においてトリアシルグリセロール合成を制御するTORキナーゼ2016

    • Author(s)
      今村壮輔、河瀬泰子、小林一幾、曾根俊之、恵良厚子、宮城島進也、下嶋美恵、太田啓之、田中寛
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンの葉緑体光応答に関するCmHIKの機能解析2016

    • Author(s)
      小倉駿佑、安藤洸幸、佐藤大地、渡辺智、藤井岳、今村壮輔、田中寛、五十嵐雅之、内海龍太郎、華岡光正
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンの概日時計応答に関する細胞内シグナル伝達の解析2016

    • Author(s)
      片野貴章、雪竹健太郎、恵良厚子、宮城島進也、藤井岳、今村壮輔、田中寛、華岡光正
    • Organizer
      第57回 日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolae(シゾン)の遺伝子改変ホスト株の分離2015

    • Author(s)
      瀧景子、曾根俊之、小林勇気、渡辺智、今村壮輔、田中寛
    • Organizer
      第38回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Overexpression of glycerol-3-phosphate acyltransferase improves TAG productivity in a unicellular red alga Cyanidioschyzon merolae2015

    • Author(s)
      Eri Hirasawa, Kan Tanaka, Sousuke Imamura
    • Organizer
      6th International Singapore Lipid Symposium/6th Asian Symposium on Plant Lipids
    • Place of Presentation
      National University of Singapore
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae2015

    • Author(s)
      Sousuke Imamura, Yasuko Kawase, Ikki Kobayashi, Toshiyuki Sone, Atsuko Era, Shin-ya Miyagishima, Mie Shimojima, Hiroyuki Ohta and Kan Tanaka
    • Organizer
      6th International Singapore Lipid Symposium/6th Asian Symposium on Plant Lipids
    • Place of Presentation
      National University of Singapore
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of cyanobacterial acyl-ACP reductase elevates the triacylglycerol level in the red alga Cyanidioschyzon merolae2015

    • Author(s)
      Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura and Shin-ya Miyagishima
    • Organizer
      6th International Singapore Lipid Symposium/6th Asian Symposium on Plant Lipids
    • Place of Presentation
      National University of Singapore
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of ribosome RNA transcription in a unicellular red alga Cyanidioschyzon merolae2015

    • Author(s)
      Sousuke Imamra、Yoshinori Fukunaga、Yuzuru Tozawa、Kan Tanaka
    • Organizer
      2nd International Symposium Matryoshka-type Evolution of Eukaryotic Cells
    • Place of Presentation
      Tsukuba
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of nitrogen deficiency responsive transcription factor MYB1 in the red alga Cyanidioschyzon merolae2015

    • Author(s)
      Takashi Kanzaki, Sousuke Imamura, Kan Tanaka
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolae におけるTOR (target of rapamycin) の機能解析2015

    • Author(s)
      今村 壮輔、田中 寛
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 単細胞紅藻シゾンにおける葉緑体に依存した核遺伝子の光誘導転写制御2015

    • Author(s)
      大原 ひかる, 安藤 洸幸, 小倉 駿佑, 藤井 岳, 今村 壮輔, 田中 寛, 恵良 厚子, 宮城島 進也, 五十嵐 雅之, 内海 龍太郎, 華岡光正
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolae における窒素同化系とその制御2014

    • Author(s)
      今村 壮輔
    • Organizer
      微細藻類研究会2014
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2014-12-22
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 藻類バイオ燃料生産に向けた分子生物学的アプローチ2014

    • Author(s)
      今村 壮輔
    • Organizer
      化学工学会 2014年度開発型企業の会 第5回(拡大)技術交流会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2014-12-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 単細胞紅藻シゾンにおけるプラスチドシグナルを介した核遺伝子の転写制御2014

    • Author(s)
      安藤洸幸、大原ひかる、小倉駿佑、藤井岳、今村壮輔、田中寛、恵良厚子、宮城島進也、五十嵐雅之、内海龍太郎、華岡光正
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 植物におけるTOR (target of rapamycin) の機能解明に向けて:ラパマイシンに感受性を示すシゾン株の構築2014

    • Author(s)
      今村壮輔、石綿愛子、渡辺智、吉川博文、田中寛
    • Organizer
      第8回 日本ゲノム微生物学会年会
    • Place of Presentation
      東京農業大学(東京)
    • Related Report
      2013 Research-status Report
  • [Presentation] Cyanidioschyzon merolaeの光応答転写制御における二成分制御系の役割2014

    • Author(s)
      安藤 洸幸、小倉 駿佑、佐藤 大地、藤井 岳、今村 壮輔、田中 寛、五十嵐 雅之、内海 龍太郎、華岡 光正
    • Organizer
      日本農芸化学会平成26年度大会
    • Place of Presentation
      明治大学(川崎)
    • Related Report
      2013 Research-status Report
  • [Presentation] ラパマイシンに感受性を示すシゾン株の構築 ―植物におけるTORキナーゼの機能解明に向けて―2013

    • Author(s)
      今村壮輔、石綿愛子、渡辺智、吉川博文、田中寛
    • Organizer
      第7回 日本ゲノム微生物学会若手の会
    • Place of Presentation
      ろうきん研修所富士センター(静岡)
    • Related Report
      2013 Research-status Report
  • [Presentation] 単細胞紅藻シゾンの葉緑体光応答と転写制御機構2013

    • Author(s)
      佐藤 大地、安藤 洸幸、小倉 駿佑、藤井 岳、今村 壮輔、田中 寛、華岡 光正
    • Organizer
      日本植物学会 第77回大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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