Investigation of seed maturation programs mediated by noncanonical Aux/IAA proteins

• Project/Area Number: 25440131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Mie University
• Principal Investigator: KAGAYA Yasuaki 三重大学, 生命科学研究支援センター, 准教授 (20335152)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 種子成熟 / 種子休眠 / アブシジン酸 / オーキシン / 転写因子 / 細胞分裂 / シロイヌナズナ / 一次休眠 / 転写制御因子

Outline of Final Research Achievements

The noncanonical Aux/IAA proteins are a group of members in the Aux/IAA family, which lack of domain II required for auxin-dependent degradation and hence are likely constitutive negative regulators of auxin-induced transcription. However, the physiological roles of the noncanonical Aux/IAA proteins remain unclear. Here, we analyzed function of these proteins in seed maturation program. We show that IAA30 is involved in the regulation of embryo growth arrest during seed maturation while IAA32 functions in acquisition of primary seed dormancy.

Research Products

• [Journal Article] The Arabidopsis transcription factor NAI1 is required for enhancing the active histone mark but not for removing the repressive mark on PYK10, a seedling–specific gene upon embryonic-to-postgerminative developmental phase transition. (2015)
  • Author(s): Yoshii, M., Yamamoto, A., Kagaya, Y., Takeda S. and Hattori, T.
  • Journal Title: Plant Signal. Behav. Volume: 10 Issue: 12 Pages: e1105418-e1105418
  • DOI: 10.1080/15592324.2015.1105418
  • Peer Reviewed
• [Journal Article] Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development. (2015)
  • Author(s): Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagawa, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori
  • Journal Title: The Plant Journal Volume: 81 Issue: 1 Pages: 1-12
  • DOI: 10.1111/tpj.12705
  • Peer Reviewed / Open Access
• [Journal Article] Cell-by-cell developmental transition from embryo to postgermination phase revealed by heterochronic gene expression and ER-body formation in Arabidopsis leafy cotyledon mutants. (2014)
  • Author(s): Yamamoto, A., Yoshii, M., Murase, S., Fujita, M., Kurata, N., Hobo, T., Kagaya, Y., Takeda, S. and Hattori, T.
  • Journal Title: Plant & Cell Physiology Volume: 55 Issue: 12 Pages: 2112-2125
  • DOI: 10.1093/pcp/pcu139
  • Peer Reviewed / Open Access
• [Presentation] 非典型型Aux/IAAタンパク質IAA30はFUS3によるABAとIAAレベルの上昇を介して種子休眠獲得に関わる (2015)
  • Author(s): 加賀谷安章、山口裕子、脇坂恵、加賀谷道子、山本章子、竹林裕美子、瀬尾光範、服部束穂
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-17 – 2015-03-18
• [Presentation] 非典型型Aux/IAAタンパク質を介した胚成長停止と休眠獲得 (2014)
  • Author(s): 加賀谷安章、脇坂恵、福田志穂、山口裕子、加賀谷道子、山本章子、服部束穂
  • Organizer: 第35回種子生理生化学研究会年会
  • Place of Presentation: 新潟市 岩室温泉 ほてる大橋館の湯
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] IAA30を介した種子休眠獲得メカニズム (2014)
  • Author(s): 山口裕子、脇坂恵、加賀谷道子、竹林裕美子、瀬尾光範、服部束穂、加賀谷安章
  • Organizer: 第35回種子生理生化学研究会年会
  • Place of Presentation: 新潟市 岩室温泉 ほてる大橋館の湯
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] 種子休眠と発芽ー胚成長の停止と再開という視点から (2014)
  • Author(s): 加賀谷安章、脇坂恵、加賀谷道子、岩田昇悟、加藤大和、山本章子、武田真、服部束穂
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学五福キャンパス
• [Presentation] 非典型Aux/IAAタンパク質IAA30は種子休眠の獲得に関わる (2014)
  • Author(s): 脇坂恵、加賀谷道子、山本章子、竹林裕美子、瀬尾光範、服部束穂、加賀谷安章
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学五福キャンパス