Analysis of ER membrane proteins in ER dynamics

• Project/Area Number: 25440132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Ueda Haruko 京都大学, 理学(系)研究科(研究院), 研究員 (90402776)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小胞体 / 原形質流動 / 膜融合 / 細胞内運動 / 植物細胞

Outline of Final Research Achievements

The endoplasmic reticulum (ER) is the organelle with the largest membrane surface area in eukaryotic cells. The ER membrane is physically continuous and extends throughout the cytoplasm. A striking feature of the plant ER is the active streaming driven by plant-specific class XI myosins. We found that the ER streaming was suppressed in root hair defective (rhd3) mutants. RHD3 is a membrane-associated dynamin-like GTPase. A deficiency of RHD3 caused a defect in the interconnection of the membranes, resulting in the occurrence of many ER fragments in the rhd3 cells. In addition, the ER network is RHD3-dependently reconstituted from the cytosol and microsome fraction of tobacco cultured cells by exogenously adding GTP, ATP, and F-actin. These results indicate that RHD3 functions as an ER membrane fusogen in plants. The fusogenic activity of RHD3 may contribute to the smooth movement of the ER.

Research Products

• [Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis. (2016)
  • Author(s): Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
  • Journal Title: Plant Physiol. Volume: 170 Issue: 2 Pages: 867-880
  • DOI: 10.1104/pp.15.01172
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Myrosin cells are differentiated directly from ground meristem cells and are developmentally independent of the vasculature in Arabidopsis leaves (2016)
  • Author(s): Shirakawa, M., Ueda, H., Shimada, T., Hara-Nishimura, I.
  • Journal Title: Plant Signal. Behav. Volume: 11 Issue: 4 Pages: e1150403-e1150403
  • DOI: 10.1080/15592324.2016.1150403
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functions of plant-specific myosin XI: from intracellular motility to plant postures. (2015)
  • Author(s): Ueda, H., Tamura, K., Hara-Nishimura, I.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 28 Pages: 30-38
  • DOI: 10.1016/j.pbi.2015.08.006
  • NAID: 120005676685
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of organ straightening and plant posture by an actin-myosin XI cytoskeleton (2015)
  • Author(s): Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Kato, T., Tasaka, M., Morita, M.T., and Hara-Nishimura, I.
  • Journal Title: Nature Plants Volume: 1 Issue: 4 Pages: 1-6
  • DOI: 10.1038/nplants.2015.31
  • NAID: 120005593639
  • Description: 【掲載確定】
  • Peer Reviewed
• [Journal Article] An ABC transporter B family protein, ABCB19, is required for cytoplasmic streaming and gravitropism of the inflorescence stems. (2015)
  • Author(s): Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Koumoto, Y., Tasaka, M., Morita, M. T., and Hara-Nishimura, I.
  • Journal Title: Plant Signal. Behav. Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Microtubules Contribute to Tubule Elongation and Anchoring of Endoplasmic Reticulum, Resulting in High Network Complexity in Arabidopsis thaliana (2014)
  • Author(s): Hamada, T., Ueda, H., Kawase, T., and Hara-Nishimura, I.
  • Journal Title: Plant Physiol Volume: 166 Issue: 4 Pages: 1869-1876
  • DOI: 10.1104/pp.114.252320
  • Peer Reviewed / Open Access
• [Journal Article] Myrosin Cell Development Is Regulated by Endocytosis Machinery and PIN1 Polarity in Leaf Primordia of Arabidopsis thaliana (2014)
  • Author(s): Makoto Shirakawa, Haruko Ueda, Tomoo Shimada, Takayuki Kohchi, and Ikuko Hara-Nishimura
  • Journal Title: The Plant Cell Volume: 26 Issue: 11 Pages: 4448-4461
  • DOI: 10.1105/tpc.114.131441
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FAMA Is an Essential Component for the Differentiation of Two Distinct Cell Types, Myrosin Cells and Guard Cells, in Arabidopsis (2014)
  • Author(s): Makoto Shirakawa, Haruko Ueda, Atsushi J. Nagano, Tomoo Shimada, Takayuki Kohchi, and Ikuko Hara-Nishimura
  • Journal Title: The Plant Cell Volume: 26 Issue: 10 Pages: 4039-4052
  • DOI: 10.1105/tpc.114.129874
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid accumulation in Arabidopsis thaliana (2014)
  • Author(s): Ichino T, Fuji K, Ueda H, Takahashi H, Koumoto Y, Takagi J, Tamura K, Sasaki R, Aoki K, Shimada T, Hara-Nishimura I.
  • Journal Title: Plant J Volume: 80 Issue: 3 Pages: 410-423
  • DOI: 10.1111/tpj.12637
  • Peer Reviewed / Open Access
• [Journal Article] COSTIMBOUS VASCPLAR RISG (COVI) is a trans-Golgi Network-Looulizde Membruoe Protein Pequirde for Golgi Morphology and Varuolur Protein Sorling (2014)
  • Author(s): Makoto Shirakawa, Haruko Heda. Yasuko Koumoto. Kentaro Fuji. Chiaki Nishiyama. Takayuki Kahchi, Ikuko Hara-Nishimura and Tomoo Shimado
  • Journal Title: Plant and Cell Physiology Volume: 55 Issue: 4 Pages: 764-772
  • DOI: 10.1093/pcp/pct195
  • Peer Reviewed
• [Journal Article] A myosin XI-I Links the Nuclear Membrane with Cytoskeleton for Nuclear Movement and Shape in Arabidopsis thaliana (2013)
  • Author(s): Tamura K, Iwabuchi K, Fukao Y, Kondo M, Okamoto K, Ueda H, Nishimura M, Hara-Nishimura I
  • Journal Title: Current Biology Volume: 23 Issue: 18 Pages: 1776-1781
  • DOI: 10.1016/j.cub.2013.07.035
  • Peer Reviewed
• [Presentation] リン酸化を介した小胞体膜融合因子RHD3の活性調節 (2016)
  • Author(s): 上田晴子、横田悦雄、桑田啓子、真野昌二、嶋田知生、田村謙太郎、深尾陽一朗、新免輝男、西村幹夫、西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-20
• [Presentation] アクチン・ミオシンXI依存的なストレートニング機構の役割 (2016)
  • Author(s): 上田晴子，岡本圭史，嶋田知生，田村謙太郎，加藤荘英，田坂昌生，森田美代，西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-19
• [Presentation] An Analysis of Transport of Glucosinolates in Arabidopsis Leaves. (2016)
  • Author(s): 中崎淳子、山田健志、河本恭子、上田晴子、西村いくこ、嶋田知生
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-19
• [Presentation] Chemical defense systems in Brassicaceae plants. (2016)
  • Author(s): Hara-Nishimura, I., Yamada, K., Shirakawa, M., Nakano, R., Ueda, H., Shimada, T.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Abscission of the outermost cell layer of Arabidopsis root cap is controlled by modification of cell wall pectin. (2016)
  • Author(s): 前田和輝、國枝正、上田晴子、嶋田知生、西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Flavonoid accumulation in Arabidopsis seeds affected by GFS9-mediated membrane trafficking (2016)
  • Author(s): Ichino, T., Fuji, K., Ueda, H., Takahashi, H., Koumoto, Y., Takagi, J., Tamura, K., Sasaki, R., Aoki, K., Schumacher, K., Shimada, T., Hara-Nishimura, I.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Active movements and network formation of the endoplasmic reticulum in plant cells (2015)
  • Author(s): Ueda, H., Yokota, E.
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（神戸市）
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] An actin-myosin XI cytoskeleton determines plant posture by regulating organ straightening. (2015)
  • Author(s): Ueda, H., Shimada, T., Tamura, K., Terao Morita, M., Hara-Nishimura, I.
  • Organizer: 8th Plant Biomechanics International Conference
  • Place of Presentation: 名古屋大学（名古屋市）
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] 植物の形態におけるミオシンXIの役割 (2015)
  • Author(s): 上田晴子
  • Organizer: 植物細胞骨格研究会2015
  • Place of Presentation: 国立遺伝学研究所（三島市）
  • Year and Date: 2015-11-27
• [Presentation] シロイヌナズナにおける小胞体に異常を示す変異体の解析 (2015)
  • Author(s): 太田奈津美，上田晴子，濱田隆宏，木森義隆，嶋田知生，山田健志，田村謙太郎，近藤真紀，亀井保博，西村幹夫，西村いくこ
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物の細胞内膜系が支える生体防御戦略 (2014)
  • Author(s): 西村いくこ, 初谷紀幸, 白川一, 上田晴子, 嶋田知生
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Quantitative analysis and intracellular machineries for streaming of endoplasmic reticulum in Arabidopsis thaliana (2014)
  • Author(s): Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Hara-Nishimura, I.
  • Organizer: 第38回内藤コンファレンス
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] Endomembrane-mediated plant defense strategies against pathogens and herbivores (2014)
  • Author(s): Hara-Nishimura, I., Hatsugai,N., Shirakawa, M., Ueda, H., and Shimada, T.
  • Organizer: 第38回内藤コンファレンス
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-07 – 2014-10-10
  • Invited
• [Presentation] 植物の細胞内ダイナミクス (2014)
  • Author(s): 上田晴子，横田悦雄，朽名夏麿，西村いくこ
  • Organizer: 第23回日本バイオイメージング学会 公開講座
  • Place of Presentation: 大阪
  • Year and Date: 2014-09-06
  • Invited
• [Book] Endoplasmic reticulum throughout the cytoplasm in "Atlas of Plant Cell Structure" (2014)
  • Author(s): Ueda, H., Yokota, E., and Hara-Nishimura, I.
  • Total Pages: 202
  • Publisher: Springer
• [Remarks] 植物の姿勢を決めるしくみの解明 －まっすぐになろうとする力－
  • URL: http://www.kyoto-u.ac.jp/ja/research/research_results/2014/150324_1.html