Regulatory mechanism of mRNA processing factors in plant morphogenesis

• Project/Area Number: 25440133
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: TSUGE Tommohiko 京都大学, 化学研究所, 准教授 (50291076)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物形態形成 / 遺伝子発現制御 / mRNA代謝制御 / スプライソソーム / タンパク質相互作用 / 転写制御 / RNAプロセシング / COP9シグナロソーム / スプライシング / タンパク質分解制御

Outline of Final Research Achievements

Six out of seven SF3b subunits in Sf3b were duplicated in Arabidopsis thaliana. Through attempts to analyze the function of each subunit, SAP130A and B, SAP14bA and B were found to be both essential for pollen development. On the other hand, SAP49A and B were found to be essential for embryogenesis. This lead us to hypothesize that, different subunits contribute to determining preferences for different sets of target genes. In addition, we discovered that csn1 partial complementary plants display pollen deformity very similar to that of SAP130 RNAi plants, This suggests a common molecular mechanism coordinately governed by CSN1 and SAP130, to regulate genes involved in pollen development.

Research Products

• [Int'l Joint Research] YALE大学(米国)
• [Int'l Joint Research] ローマ大学(イタリア)
• [Int'l Joint Research] 北京大学(中国)
• [Journal Article] RNA binding proteins RZ-1B and RZ-1C play critical roles in regulating pre-mRNA splicing and gene expression during development in Arabidopsis. (2016)
  • Author(s): Wu Z, Zhu D, Lin X, Miao J, Gu L, Deng X, Yang Q, Sun K, Zhu D, Cao X, Tsuge T, Dean C, Aoyama T, Gu H, Qu LJ.
  • Journal Title: Plant Cell Volume: 28 Issue: 1 Pages: 55-73
  • DOI: 10.1105/tpc.15.00949
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] UV-B-Induced CPD photolyase gene expression is regulated by UVR8-dependent and -independent pathways in Arabidopsis. (2015)
  • Author(s): Li N, Teranishi M, Yamaguchi Ｈ, Matsushita T, Watahiki MK, Tsuge T, Li SS, Hidema J
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 10 Pages: 2014-2023
  • DOI: 10.1093/pcp/pcv121
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana. (2015)
  • Author(s): Wada, Y., Kusano, H., Tsuge, T., and Aoyama, T.
  • Journal Title: Plant J. Volume: 81 Issue: 3 Pages: 426-437
  • DOI: 10.1111/tpj.12741
  • Peer Reviewed / Open Access
• [Journal Article] GLABRA2 directly suppresses basic helix-loop-helix transcription factor genes with diverse functions in root hair development. (2015)
  • Author(s): Lin, Q., Ohashi, Y., Kato, M., Tsuge, T., Gu ,H., Qu, L.-J., and Aoyama, T.
  • Journal Title: Plant Cell Volume: 27 Pages: 2894-2906
  • DOI: 10.1105/tpc.15.00607
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ADP1 affects plant architecture by regulating local auxin biosynthesis. (2014)
  • Author(s): Li R, Li J, Li S, Qin G, Novak O, Pencik A, Ljung K, Aoyama T, Liu J, Murphy A, Gu H, *Tsuge T, *Qu LJ.
  • Journal Title: PLoS Genet Volume: 10 Issue: 1 Pages: e1003954-e1003954
  • DOI: 10.1371/journal.pgen.1003954
  • Peer Reviewed
• [Journal Article] The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 protein forms an SCF ubiquitin ligase and regulates hypocotyl elongation (2013)
  • Author(s): Franciosini A, Lombardi B, Iafrate S, Pecce V, Mele G, Lupacchini L, Kondou Y, Gusmaroli G, Aki S, Tsuge T, Deng XW, Matsui M, Vittorioso P, Costantino P, Serino G
  • Journal Title: Mol Plant Volume: Mar 9. Epub ahead of print Issue: 5 Pages: 1616-1629
  • DOI: 10.1093/mp/sst045
  • Peer Reviewed
• [Presentation] シロイヌナズナにおけるCSN相互作用因子CPSF6の機能解析 (2016)
  • Author(s): 張暁娟、青山卓史、柘植知彦
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] 植物細胞形態形成における PIP5K 遺伝子の機能重複性と制御的役割 (2016)
  • Author(s): 亘真智子、Blanc-Mathieu Romain、藤真理子、柘植知彦、緒方博之、青山卓史
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナ PLDζ2 の細胞生物学的機能 (2016)
  • Author(s): 島村亮太、谷口(山本)幸美、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] COP9 signalosome: Understanding its role as a modulator linking proteolysis to RNA processing. (2016)
  • Author(s): Tomohiko TSUGE
  • Organizer: New era of pre-mRNA splicing world – satellite meeting
  • Place of Presentation: Hotel Nikko Nara, Nara JAPAN
  • Year and Date: 2016-03-14
  • Invited
• [Presentation] COP/DET/FUSを介した選択的スプライシングの制御系の解析に向けて (2016)
  • Author(s): 黒田凌、後藤翔、野元美佳、青山卓史、多田安臣、古本強、松下智直、柘植知彦
  • Organizer: 第５回植物RNA研究者ネットワークシンポジウム
  • Place of Presentation: 東京大学駒場キャンパス 21 KOMCEE、東京都目黒区
  • Year and Date: 2016-01-08
• [Presentation] Functional analyses of a CSN interacting protein, CPSF6, in Arabidopsis thaliana (2016)
  • Author(s): Xiaojuan ZHANG, Takashi AOYAMA, Tsuyoshi FURUMOTO, Tom TSUGE
  • Organizer: 第５回植物RNA研究者ネットワークシンポジウム
  • Place of Presentation: 東京大学駒場キャンパス 21 KOMCEE、東京都目黒区
  • Year and Date: 2016-01-08
• [Presentation] CULLIN neddylation is regulated during seed development in Arabidopsis thaliana. (2015)
  • Author(s): Anna FRANCIOSINI, Simone BUTERA, Niccolò MOSESSO, Isabelle BONOMO, Alessandra BOCCACCINI, Tomohiko TSUGE, Maura CARDARELLI, Paola VITTORIOSO, Paolo COSTANTINO, Giovanna SERINO
  • Organizer: The 26th International Conference on Arabidopsis Research
  • Place of Presentation: Palais des Congrès de Paris, Paris FRANCE
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] COP9シグナロソーム結合因子AtPrp43の機能解析 (2015)
  • Author(s): 後藤翔*、野元美佳、多田安臣、古本強、青山卓史、柘植知彦
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Functional analyses of CPSF6 in Arabidopsis thaliana (2015)
  • Author(s): Xiaojuan ZHANG*, Tsuyoshi FURUMOTO, Takashi AOYAMA, Tomohiko TSUGE
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナ根毛におけるCa2+結合タンパク質PCaP2とホスファチジルイノシトール4,5-二リン酸の細胞内動態 (2015)
  • Author(s): 加藤真理子*、柘植知彦、前島正義、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] PIP5K3と他のタイプB PIP5K遺伝子との機能重複に関する解析 (2015)
  • Author(s): 齊藤涼*、和田悠貴香、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 側方根幹におけるPLDζ2の役割 (2015)
  • Author(s): 島村亮太*、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、林謙一郎、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Gene expression of CPD photolyase is primarily mediated by UVR8- and cryptochrome-dependent pathways in de-etiolated Arabidopsis seedlings (2015)
  • Author(s): Nan LI*, Mika TERANISHI, Tomonao MATSUSHITA, Tomohiko TSUGE, Masaaki WATAHIKI, Jun HIDEMA
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Functional characterization of COP9 signalosome interacting factor AtPrp43 (2015)
  • Author(s): Kakeru GOTO*, Mika NOMOTO, Yasuomi TADA, Takashi AOYAMA, Tsuyoshi FURUMOTO, Tomohiko TSUGE
  • Organizer: The 2nd International Symposium on Plant Environmental Sensing
  • Place of Presentation: AIST Tokyo Waterfront Annex, Koto-ku TOKYO
  • Year and Date: 2015-03-13 – 2015-03-15
• [Presentation] スプライソソーム構成因子群とCOP9シグナロソームが植物形態形成に担う役割を考える (2015)
  • Author(s): 安喜史織、張俊、後藤翔、中井秀人、青山卓史、瞿礼嘉、柘植知彦*
  • Organizer: 第４回植物RNA研究者ネットワークシンポジウム RNAから植物を考える −植物を支えるRNA機能—
  • Place of Presentation: 京都大学理学部セミナーハウス、京都府京都市
  • Year and Date: 2015-01-19 – 2015-01-20
  • Invited
• [Presentation] COP9シグナロソーム結合因子AtPrp43の機能解析 (2015)
  • Author(s): 後藤翔*、野元美佳、多田安臣、古本強、青山卓史、柘植知彦
  • Organizer: 第４回植物RNA研究者ネットワークシンポジウム RNAから植物を考える -植物を支えるRNA機能-
  • Place of Presentation: 京都大学理学部セミナーハウス、京都府京都市
  • Year and Date: 2015-01-19 – 2015-01-20
• [Presentation] Functional analyses of CPSF6 in Arabidopsis thaliana (2015)
  • Author(s): Xiaojuan ZHANG*, Tsuyoshi FURUMOTO, Takashi AOYAMA, Tomohiko TSUGE
  • Organizer: 第４回植物RNA研究者ネットワークシンポジウム RNAから植物を考える -植物を支えるRNA機能-
  • Place of Presentation: 京都大学理学部セミナーハウス、京都府京都市
  • Year and Date: 2015-01-19 – 2015-01-20
• [Presentation] 根毛伸長時におけるシロイヌナズナCa2+結合タンパク質PCaP2とホスファチジルイノシトール4,5-二リン酸の細胞内動態 (2014)
  • Author(s): 加藤真理子*、柘植知彦、前島正義、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市山岳交流センター、静岡県静岡市
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] シロイヌナズナの根毛伸長におけるPIP5K遺伝子の役割 (2014)
  • Author(s): 和田悠貴香、草野博彰、亘真智子、齊藤涼、加藤真理子、柘植知彦、青山卓史*
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市山岳交流センター、静岡県静岡市
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] シロイヌナズナの側方根幹におけるPLDζ2-PAシグナルの役割 (2014)
  • Author(s): 島村亮太*、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市山岳交流センター、静岡県静岡市
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] The role of ARF8 and ARF6 in late stamen development in Arabidopsis. (2014)
  • Author(s): Valentina CECCHETTI, Patrizia BRUNETTI, Roberta GHELLI, Angelo DE PAOLIS, Tomohiko TSUGE, Paolo COSTANTINO, Maura CARDARELLI*
  • Organizer: EMBO Conference -Interdisciplinary plant development-
  • Place of Presentation: Sainsbury Laboratory University of Cambridge, Cambridge UNITED KINGDOM
  • Year and Date: 2014-09-21 – 2014-09-24
• [Presentation] スプライソソーム構成因子群と CSN は植物形態形成に重要な働きを担う (2014)
  • Author(s): 張俊、安喜史織、後藤翔、中井秀人、青山卓史、瞿礼嘉、柘植知彦
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] AtPrp43 RNA ヘリカーゼの機能解析 (2014)
  • Author(s): 後藤翔、青山卓史、柘植知彦
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] COP9 シグナロソーム相互作用する Trihelix protein の機能解析 (2014)
  • Author(s): 中井秀人*、安喜史織、HEYL Alexander、青山卓史、柘植知彦
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] シロイヌナズナ PLDzeta1 による細胞内情報伝達 (2014)
  • Author(s): 島村亮太、安斎尚子、加藤真理子、谷口雅俊、大橋洋平、柘植知彦、青山卓史
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] スプライソソーム構成因子群とCOP9シグナロソームは植物形態形成に重要な役割を担う (2013)
  • Author(s): 安喜史織、張俊、後藤翔、中井秀人、青山卓史、瞿礼嘉、柘植知彦
  • Organizer: RNAから植物を考えるー植物をささえるRNA機能ー
  • Place of Presentation: 北海道大学、北海道札幌市
  • Invited
• [Presentation] Understanding CSN mediated regulation through its binding partners: linking proteolysis to RNA processing. (2013)
  • Author(s): Tomohiko TSUGE*
  • Organizer: Invited lecture at “The 6th Asia & Oceania Conference on Photobiology”
  • Place of Presentation: Novotel Sydney Central, Sydney AUSTRALIA.
  • Invited
• [Presentation] COP9 signalosome: Understanding its role as a modulator linking proteolysis to RNA processing. (2013)
  • Author(s): Tomohiko TSUGE*
  • Organizer: Invited lecture at “The 3rd International Symposium on Integrative Plant Biology”
  • Place of Presentation: Lijiang, PRC.
  • Invited
• [Book] 光シグナル伝達とCOPタンパク質「光と生命の事典」 (2016)
  • Author(s): 柘植知彦
  • Publisher: 朝倉書店
• [Remarks] 京都大学生体分子情報研究領域ホームページ
  • URL: http://www.scl.kyoto-u.ac.jp/~molbio/index.html