Analysis of the phospho-signaling pathway leading to microtubule depolymerization from osmotic stress in higher plants

• Project/Area Number: 25440135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Takehide Kato 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (70379535)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: シロイヌナズナ / 微小管 / チューブリン / リン酸化 / 細胞骨格 / 高浸透圧 / 植物 / シグナル伝達

Outline of Final Research Achievements

The dynamic change of higher microtubule structures is effected by not only a cell cycle control but also several environmental stimulus. PHS1 is involved in microtubule formation or maintenance. When plant cells are given by the hyper osmotic stress, alpha-tubulins are phosphorylated, resulting into promoting microtubule depolymerization. In this study, it was elucidated that Thr349 of alpha-tubulin is the target of PHS1. Next, we isolated the fifteen phosphorylation sites that response to the hyper osmotic stimulus. Moreover, it is suggested that the microtubule depolymerization via PHS1 is related with induction of the M-phase check point, and it is important for safely segregating of chromosomes in the dividing cell under hyper osmotic stress.

Research Products

• [Journal Article] Regulation of organ straightening and plant posture by an actin-myosin XI cytoskeleton (2015)
  • Author(s): Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Kato, T., Tasaka, M., Morita, M.T., and Hara-Nishimura, I.
  • Journal Title: Nature Plants Volume: 1 Issue: 4 Pages: 1-6
  • DOI: 10.1038/nplants.2015.31
  • NAID: 120005593639
  • Description: 【掲載確定】
  • Peer Reviewed
• [Journal Article] A unique HEAT repeat-containing protein SHOOT GRAVITROPISM 6 is involved in vacuolar membrane dynamics in gravity sensing cells of Arabidopsis inflorescence stem (2014)
  • Author(s): Hashiguchi, Y., Yano, D., Nagafusa, K., Kato, T., Saito, C., Uemura, T., Ueda, T., Nakano, A., Tasaka, M. and Morita, MT
  • Journal Title: Plant Cell Phys. Volume: 55 Issue: 4 Pages: 811-822
  • DOI: 10.1093/pcp/pcu020
  • Peer Reviewed
• [Journal Article] Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures. (2013)
  • Author(s): Takahiro Hamada, Nahoko Nagasaki Tekeuchi, Takehide Kato, Masayuki Fujiwara, Seiji Sonobe, Yoichiro Fukao, Takashi Hashimoto
  • Journal Title: Plant Physiol. Volume: 163 Issue: 4 Pages: 1804-1816
  • DOI: 10.1104/pp.113.225607
  • Peer Reviewed
• [Journal Article] An atypical tubulin kinase mediates stress-induced microtubule depolymer ization in Arabidopsis (2013)
  • Author(s): Satoshi Fujita, Jaromir Pytela, Takashi Hotta, Takehide Kato, Takahiro Hamada, Rie Akamatsu, Yasumasa Ishida, Natsumaro Kutsuna, Seiichiro Hasezawa, Yuko Nomura, Hirofumi Nakagami, Takashi Hashimoto
  • Journal Title: Curr. Biol Volume: 23 Issue: 20 Pages: 1969-1978
  • DOI: 10.1016/j.cub.2013.08.006
  • Peer Reviewed
• [Journal Article] Amyloplast displacement is necessary for gravisensing in Arabidopsis shoots as revealed by a centrifuge microscope. (2013)
  • Author(s): Toyota M, Ikeda N, Sawai-Toyota S, Kato T, Gilroy S, Tasaka M, Morita MT.
  • Journal Title: The Plant Journal Volume: 76(4) Issue: 4 Pages: 648-660
  • DOI: 10.1111/tpj.12324
  • Peer Reviewed
• [Presentation] 器官のねじれ形質への各細胞層の寄与 (2016)
  • Author(s): 加藤壮英、市村朋子、平井祐貴、足立侑実子、寳金佐和子、藤田智史、八木慎宜、橋本隆
  • Organizer: 第57回 日本植物生理学会年会
  • Place of Presentation: 岩手大学 上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] アクチン・ミオシンXI依存的なス トレートニング機構の役割 (2016)
  • Author(s): 上田晴子、岡本圭史、嶋田知生、田村謙太郎、加藤壮英、田坂昌生、森田美代、西村いくこ
  • Organizer: 第57回 日本植物生理学会年会
  • Place of Presentation: 岩手大学 上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Regulation of microtubule stability by tubulin phosphorylation is related to osmotic stress response in Arabidopsis. (2014)
  • Author(s): Takehide Kato
  • Organizer: Japanese San Francisco Bay Area Seminar Annual Meeting 2014
  • Place of Presentation: Genentech Hall, UCSF Mission Bay Campus, San Francisco, USA
  • Year and Date: 2014-10-18
• [Presentation] Functional analysis of novel Arabidopsis proteins that associate with microtubule nucleation sites (2014)
  • Author(s): Noriyoshi Yagi，Takahiro Hamada，Masayoshi Nakamura，Mayumi Kawaguchi，Takehide Kato, Takashi Hashimoto
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山大学 五福キャンパス（富山県富山市）
• [Presentation] 環境ストレスに応答する微小管制御 (2013)
  • Author(s): 藤田 智史、Jaromir Pytela、堀田 崇、 加藤 壮英、濱田 隆宏、Wong Jeh-Haur、 朽名 夏麿、 野村 有子、馳澤 盛一郎、 中神 弘史、橋本 隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学 高等教育推進機構（北海道札幌市）
• [Book] Cleaved Amplified Polymorphic Sequences (CAPS) Markers in Plant Biology (2014)
  • Author(s): Takehide Kato, Masatsugu Toyota, Masao Tasaka and Miyo Terao Morita
  • Total Pages: 253
  • Publisher: Nova Science Publishers, Inc.