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Regulation of organ growth by synthesis of very-long-chain fatty acids in Arabidopsis

Research Project

Project/Area Number 25440136
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Okushima Yoko  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (00432592)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords器官成長 / シロイヌナズナ / 脂質 / 植物ホルモン / 脂肪酸
Outline of Final Research Achievements

In plants, very-long-chain fatty acids (VLCFAs) are used for the synthesis of triacylglycerols, cuticular waxes and sphingolipids. Recently we have revealed that VLCFA synthesis in the shoot epidermis suppresses cell proliferation in internal tissues by down-regulating cytokinin biosynthesis in the vasculature, thereby restricting aerial organ growth. However, the mechanism how VLCFA synthesis controls cytokinin biosynthesis in a non-autonomous manner remains largely elusive. In this study, we found that brassinosteroid signals are involved in VLCFA-mediated plant growth regulation. In addition, by the genetic screening of factors working in VLCFA-mediated growth regulation, we identified 5 lines of defective in cafenstrole response (dcr) mutants. Candidates of the responsible genes for two dcr mutants have been identified by the whole genome sequencing.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Differential regulation of B2-type CDK accumulation in Arabidopsis roots2014

    • Author(s)
      Okushima, Y., Shimizu, K., Ishida, T., Sugimoto, K. and Umeda, M.
    • Journal Title

      Plant Cell Rep.

      Volume: 33 Issue: 7 Pages: 1033-1040

    • DOI

      10.1007/s00299-014-1581-z

    • NAID

      120006537389

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species.2014

    • Author(s)
      Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L.
    • Journal Title

      Plant Cell

      Volume: 26(1) Pages: 296-309

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation.2013

    • Author(s)
      Nobusawa T, Okushima Y, Nagata N, Kojima M, Sakakibara H, Umeda M.
    • Journal Title

      PLoS Biol.

      Volume: 11(4)

    • NAID

      120005716835

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Restriction of cell proliferation in internal tissues via the synthesis of very-long-chain fatty acids in the epidermis.2013

    • Author(s)
      Nobusawa T, Okushima Y, Nagata N, Kojima M, Sakakibara H, Umeda M.
    • Journal Title

      Plant Signal Behav.

      Volume: 8(8)

    • Related Report
      2013 Research-status Report
  • [Journal Article] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots.2013

    • Author(s)
      Takahashi N, Kajihara T, Okamura C, Kim Y, Katagiri Y, Okushima Y, Matsunaga S, Hwang I, Umeda M.
    • Journal Title

      Curr Biol.

      Volume: 23(18) Pages: 1812-1817

    • NAID

      120005716839

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナにおいてDNA損傷応答に関わる新規因子の同定2015

    • Author(s)
      奥島 葉子、史 佳卉、奥田 利美、服部 弘嗣、坂本 智昭、倉田哲也、稲垣 宗一、梅田 正明
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regulation of organ growth by synthesis of very-long-chain fatty acids in Arabidopsis2015

    • Author(s)
      Yoko Okushima, Masaaki Umeda
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Control of shoot growth by epidermis-derived signals in Arabidopsis2015

    • Author(s)
      Yoko Okushima, Takashi Nobusawa, Masaaki Umeda
    • Organizer
      Plant Organ Growth Symposium
    • Place of Presentation
      Aula Ghent University (Ghent, Belgium)
    • Year and Date
      2015-03-10 – 2015-03-12
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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