Analysis of cell-cell interaction involved in vascular differentiation

• Project/Area Number: 25440137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Okayama University
• Principal Investigator: Hiroyasu Motose 岡山大学, 自然科学研究科, 准教授 (70342863)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI TAKU 岡山大学, 大学院自然科学研究科, 教授 (20271710)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 維管束 / 木部分化 / 糖タンパク質 / ポリアミン / サーモスペルミン / 維管束形成 / 葉酸 / 植物ホルモン / 細胞間相互作用 / アラビノガラクタンタンパク質

Outline of Final Research Achievements

We identified a transport pathway of xylogen from golgi to the plasma membrane. Furthermore, several xylogen interactors were determined. We developed a inhibitor of thermospermine biosynthesis and named it xylemin (Yoshimoto et al. 2016). Addition of xylemin and auxin analog strongly promotes xylem differentiation in various plants.

Research Products

• [Int'l Joint Research] University of British Columbia(Canada)
• [Int'l Joint Research] ENS-Lyon(France)
• [Journal Article] Chemical control of xylem differentiation by thermospermine, xylemin, and auxin. (2016)
  • Author(s): K. Yoshimoto, H. Takamura, I. Kadota, H. Motose and T. Takahashi
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 21487-21487
  • DOI: 10.1038/srep21487
  • NAID: 120005728629
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure, function, and evolution of plant NIMA-related kinases: Implication for phosphorylation-dependent microtubule regulation (2015)
  • Author(s): Takatani S, Otani K, Kanazawa M, Takahashi T, Motose H
  • Journal Title: J. Plant Research Volume: 128 Issue: 6 Pages: 875-891
  • DOI: 10.1007/s10265-015-0751-6
  • NAID: 120005703796
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Abscisic acid induces ectopic outgrowth in epidermal cells through cortical microtubule reorganization in Arabidopsis thaliana (2015)
  • Author(s): Takatani S, Hirayama T, Hashimoto T, Takahashi T, Motose H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11364-11364
  • DOI: 10.1038/srep11364
  • NAID: 120005651882
  • Peer Reviewed / Open Access
• [Journal Article] Thermospermine modulates expression of auxin-related genes in A rabidopsis (2014)
  • Author(s): Wurina Tong
  • Journal Title: Frontiers in Plant Metabolism and Chemodiver sity Volume: (印刷中) Pages: 94-94
  • DOI: 10.3389/fpls.2014.00094
  • Peer Reviewed / Open Access
• [Journal Article] Hayashi K, Nakamura S, Fukunaga S, Nishimura T, Jenness MK, Murphy AS, Motose H, Nozaki H, Furutani M, Aoyama T. (2014)
  • Author(s): Auxin transport sites are visualized in planta using fluorescent auxin analogs.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 31 Pages: 111557-111562
  • DOI: 10.1073/pnas.1408960111
  • Peer Reviewed / Open Access
• [Journal Article] Determination of polyamines in Arabidopsis thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent (2013)
  • Author(s): Genki Inoue, Takashi Kaneta, Toshio Takayanagi, Junichi Kakehi, Hiroyasu Motose, Taku Takahashi
  • Journal Title: Analytical Methods Volume: (in press) Issue: 11 Pages: 2854-2859
  • DOI: 10.1039/c3ay26360f
  • NAID: 120005285276
  • Peer Reviewed
• [Presentation] ゼニゴケNIMA関連キナーゼは仮根細胞の伸長方向を制御する (2016)
  • Author(s): 大谷健人、石崎公庸、西浜竜一、河内孝之、高橋卓、本瀬宏康
  • Organizer: 日本植物生理学会２０１６
  • Place of Presentation: 岩手
  • Year and Date: 2016-03-18
• [Presentation] Arabidopsis NEK6 depolymerizes cortical microtubules through tubulin phosphorylation to direct cell elongation (2015)
  • Author(s): Takatani S, Ozawa S, Yagi N, Hotta T, Takahashi Y, Hashimoto T, Takahashi T, Motose H
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium 2014
  • Place of Presentation: 名古屋
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] Arabidopsis NEK6 depolymerizes cortical microtubules by tubulin phosphorylation during directional cell growth (2015)
  • Author(s): Takatani S, Ozawa S, Yagi N, Hotta T, Takahashi Y, Hashimoto T, Takahashi T, Motose H
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] 維管束分化のケミカルバイオロジー (2015)
  • Author(s): 本瀬宏康
  • Organizer: 岡山大学と慶應義塾大学の若手交流シンポジウム －化学と生物学の融合を目指して－
  • Place of Presentation: 横浜
  • Year and Date: 2015-06-26
  • Invited
• [Presentation] シロイヌナズナNEKファミリーの機能解析 (2015)
  • Author(s): 金澤まい、酒井達也、高橋卓、本瀬宏康
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナNIMA関連キナーゼ6はチューブリンをリン酸化し、細胞成長を制御する (2015)
  • Author(s): 高谷彰吾，小澤真一郎，八木慎宜，堀田崇, 高橋裕一郎，橋本隆，高橋卓，本瀬宏康
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ゼニゴケNIMA関連キナーゼの機能解析 (2015)
  • Author(s): 竹田健人、石崎公庸、河内孝之、高橋卓、本瀬宏康
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 維管束分化のケミカルバイオロジー (2015)
  • Author(s): 本瀬宏康、吉本香織、小林千華、林謙一郎、能年義輝、高村浩由、門田功、高橋卓
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
  • Invited
• [Presentation] Function of NIMA-related kinases in cell growth and division in Arabidopsis thaliana (2014)
  • Author(s): H. Motose, S. Takatani, N. Yagi, T. Hashimoto, S. Ozawa, Y. Takahashi and T. Takahashi
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: Kobe
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] Functional analysis of a single NIMA-related kinase in Marchantia polymorpha (2014)
  • Author(s): K. Takeda, K. Ishizaki, T. Kohchi, T. Takahashi and H. Motose
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: Kobe
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] The roles of NIMA-related kinases on stress response and gene expression in Arabidopsis thaliana (2014)
  • Author(s): M. Kanazawa, H. Ito, H. Nishimura, T. Hirayama, T. Takahashi and H. Motose
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: Kobe
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] Abscisic acid induces ectopic outgrowth and cortical microtubule reorganization in epidermal cells of Arabidopsis thaliana. (2014)
  • Author(s): S. Takatani, T. Hirayama, T. Hashimoto, T. Takahashi and H. Motose
  • Organizer: 25th International Conference on Arabidopsis Research
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Tubulin phosphorylation by NIMA-related kinases is involved in cell growth and division. (2014)
  • Author(s): H. Motose, S. Takatani, T. Sakai, S. Ozawa, Y. Takahashi and T. Takahashi
  • Organizer: 25th International Conference on Arabidopsis Research
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] シロイヌナズナの道管分化に対するサーモスペルミン合成阻害剤の効果 (2014)
  • Author(s): 吉本香織、高村浩由、門田攻、本瀬宏康、高橋卓
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] シロイヌナズナのSAC51 mRNAの翻訳を促進するサーモスペルミンの作用機構 (2014)
  • Author(s): 高野綾香、石塚壮一、蔡青青、本瀬宏康、高橋卓
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] NIMA関連キナーゼによるチューブリンのリン酸化は細胞成長に関与する (2014)
  • Author(s): 本瀬宏康、高谷彰吾、酒井達也、小澤真一郎、高橋裕一郎、高橋卓
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] アブシジン酸は表皮細胞の突起形成と微小管の脱重合を引き起こす (2014)
  • Author(s): 高谷彰吾、橋本隆、平山隆志、高橋卓、本瀬宏康
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] NIMA関連キナーゼは微小管形成と細胞成長パターンを制御する (2013)
  • Author(s): 本瀬宏康
  • Organizer: 第77回 日本植物学会年会
  • Place of Presentation: 札幌 北海道大学
• [Remarks] 植物の木部分化を促進する生理活性物質ザイレミンを開発（プレスリリース）
  • URL: http://www.okayama-u.ac.jp/tp/release/release_id370.html
• [Remarks] 植物発生研究室
  • URL: http://www.biol.okayama-u.ac.jp/takahashiTaku/T-Takahashi.html
• [Remarks] 本瀬宏康のホームページ
  • URL: http://www15.atwiki.jp/motosehiroyasu