Physiological significance of UDP-N-acetylglucosamine biosynthesis in plants
| Project/Area Number |
25440139
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Ehime University |
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Principal Investigator |
Sato Yasushi 愛媛大学, 理工学研究科, 准教授 (80274306)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYAMA Munetaka 東京大学, 大学院理学(系)研究科, 准教授 (50202130)
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| Research Collaborator |
NOZAKI Mamoru
FURO Keisuke
NAKATSUMA Naoto
MURASHIGE Hiroshi
KOMATSU Junpei
TANAKA Masaki
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | シロイヌナズナ / lig変異体 / UDP-N-アセチルグルコサミン / 小胞体ストレス応答(UPR) / リグニン化 / GlcNAcキナーゼ / タンパク質糖鎖修飾 / 温度感受性変異体 / 小胞体ストレス応答 |
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| Outline of Final Research Achievements |
UDP-N-acetylglucosamine (UDP-GlcNAc) is essential for N-glycosylation of proteins in eukaryotes. In this study, we progressed the investigation of significance of synthesis, salvage pathway and effects of deficiency of UDP-GlcNAc in plants. For the purpose, we used various Arabidopsis mutants and transformants such as lig, in which de novo synthesis of UDP-GlcNAc was inhibited.
As a result, we novelly revealed a GlcNAc kinase in plants, which is essential for salvage synthesis of UDP-GlcNAc from GlcNAc derived from degradation of glycoproteins. Furthermore, we obtained a new knowledge about relationship between cell-wall damage, endoplasmic reticulum (ER) stress and unfolded protein response (UPR) during the process from UDP-GlcNAc deficiency to growth suppression and ectopic lignification in roots.
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Report
(4 results)
Research Products
(11 results)
