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Studies on molecular mechanism underlying photoperiodic compensation

Research Project

Project/Area Number 25440141
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionInternational Christian University

Principal Investigator

MIZOGUCHI Tsuyoshi  国際基督教大学, 教養学部, 教授 (70281623)

Co-Investigator(Kenkyū-buntansha) 楢本 悟史  東北大学, 生命科学研究科, 助教 (30612022)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords光合成 / 葉緑体 / 概日時計 / LHY / CCA1 / シロイヌナズナ / 光周性 / 既日時計 / クロロフィル / 補償性
Outline of Final Research Achievements

Circadian clock ensures consistency in antenna size under different photoperiod conditions. We named this novel clock function “photoperiodic compensation.” Double loss of function of LHY and CCA1 (lhy;cca1) resulted in changes in the total chlorophyll (Chl) level and Chl a/b ratio depending on the photoperiod. Under continuous light conditions (LL), the total Chl level increased significantly and the Chl a/b ratio decreased in lhy;cca1 compared with those in the wild type (WT). Increased stacked grana thylakoid membranes in chloroplasts were also observed in lhy;cca1 under LL. LIGHT HARVESTING COMPLEX II, which is an antenna protein involved in photosynthesis, accumulated to high levels in lhy;cca1 under LL compared with the WT.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (27 results)

All 2016 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Int'l Joint Research: 11 results,  Invited: 1 results)

  • [Int'l Joint Research] マックスプランク研究所(ドイツ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Density effects on late flowering mutants of <i>Arabidopsis thaliana</i> under continuous light2016

    • Author(s)
      Yamamoto K, Takahashi K, Hara M, Miyata K, Hayama R, Mizoguchi T*
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 4 Pages: 323-331

    • DOI

      10.5511/plantbiotechnology.16.0622a

    • NAID

      130005281093

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Density effects on semi-dwarf and early flowering mutants of <i>Arabidopsis thaliana</i> under continuous light2016

    • Author(s)
      Takahashi K, Hara M, Miyata K, Hayama R, Mizoguchi T*
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 4 Pages: 333-339

    • DOI

      10.5511/plantbiotechnology.16.0912a

    • NAID

      130005281091

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A loss-of-function mutation in the <i>DWARF4</i>/<i>PETANKO5</i> gene enhances the late-flowering and semi-dwarf phenotypes of the <i>Arabidopsis</i> clock mutant <i>lhy-12;cca1-101</i> under continuous light without affecting <i>FLC</i> expression2016

    • Author(s)
      Suzuki S, Miyata K, Hara M, Niinuma K, Tsukaya H, Takase M, Hayama R, and Mizoguchi T
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 4 Pages: 315-321

    • DOI

      10.5511/plantbiotechnology.16.0601a

    • NAID

      130005281084

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Environmental responses of plants: Biological interactions in the homogenous population or community (mixed populations)2016

    • Author(s)
      Mizoguchi T*, Funabashi M.
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 4 Pages: 211-212

    • DOI

      10.5511/plantbiotechnology.16.0000p

    • NAID

      130005281055

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The <i>FT-like</i> gene <i>PehFT</i> in petunia responds to photoperiod and light quality but is not the main gene promoting light quality-associated flowering2016

    • Author(s)
      Tsukamoto A, Hirai T, Chin DP, Mii M, Mizoguchi T*, Mizuta D, Yoshida H, Olsen J, Ezura H, Fukuda N.
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 4 Pages: 297-307

    • DOI

      10.5511/plantbiotechnology.16.0620a

    • NAID

      130005281092

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EMS mutagenesis and characterization of <i>Brassica rapa</i> mutants2014

    • Author(s)
      Mizuno, Y., Okamoto, S., Hara, M., Mizoguchi, T.
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 2 Pages: 185-190

    • DOI

      10.5511/plantbiotechnology.14.0109b

    • NAID

      130004435283

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] はじめに:GarnerとAllardが100年前に抱いた疑問, 特集:植物の概日時計と光周性花成応答2014

    • Author(s)
      溝口剛
    • Journal Title

      植物の生長調節 Regulation of Plant Growth & Development

      Volume: 49 Pages: 25-26

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 長日植物シロイヌナズナの概日時計遺伝子変異が及ぼす光周性花成応答への影響, 特集:植物の概日時計と光周性花成応答2014

    • Author(s)
      溝口剛
    • Journal Title

      植物の生長調節 Regulation of Plant Growth & Development

      Volume: 49 Pages: 27-33

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increase in vascular pattern complexity caused by mutations in <i>LHY</i> and <i>CCA1</i> in <i>Arabidopsis thaliana</i> under continuous light2014

    • Author(s)
      Aihara, K, Naramoto, S, Hara, M, Mizoguchi, T
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 1 Pages: 43-47

    • DOI

      10.5511/plantbiotechnology.13.1015a

    • NAID

      130003391361

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Late flowering phenotype under ultra-short photoperiod (USP) in Arabidopsis thaliana2014

    • Author(s)
      Sugiyama, H, Natsui, Y, Hara, M, Miyata, K, Nefissi, R, Mizoguchi, T
    • Journal Title

      Plant Biotechnology

      Volume: 31 Pages: 29-34

    • NAID

      130003391362

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] <i>CO-EXPRESSED WITH CLOCK GENES LHY AND CCA1 1</i> (<i>CEC1</i>) is regulated by LHY and CCA1 and plays a key role in phase setting of <i>GI</i> in <i>Arabidopsis thaliana</i>2014

    • Author(s)
      Hara, M, Kamada, H, Mizoguchi, T
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 1 Pages: 35-41

    • DOI

      10.5511/plantbiotechnology.13.1108a

    • NAID

      130003391363

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Circadian rhythm gating of transcription factors implicated in salinity stress in Solanum lycopersicum2016

    • Author(s)
      Coyne K, Hayama R, Mullen DM, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gibberellic acid and PTA7 may control flowering time via a common genetic pathway in Arabidopsis thaliana.2016

    • Author(s)
      Miyata K, Kobayashi M, Murayama Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Natural variation in the photoperiodic response of elf3;gi between Landsberg erecta and Columbia ecotypes in Arabidopsis thaliana.2016

    • Author(s)
      Murayama Y, Kuroki K, Sakamoto S, Fujiwara S, Hara M, Natsui Y, Takahashi K, Miyata K, Hayama R, Ito E, Choi SW, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of night break on flowering time and FT expression in elf3;gi, an SDP-type mutant of Arabidopsis thaliana.2016

    • Author(s)
      Nakai H, Hara M, Takahashi K, Ariga H, Murayama Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inversion of photoperiodic response by double loss-of-function mutations in EARLY FLOWERING 3 and GIGANTEA in Arabidopsis thaliana.2016

    • Author(s)
      Hara M, Natsui Y, Murayama Y, Kuroki K, Takahashi K, Miyata K, Chiba Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of SELF PRUNING 5G in the floral and sympodial regulation in a day-neutral plant tomato.2016

    • Author(s)
      Kato K, Oikawa J, Ishigami Y, Shimoyama S, Shiba T, Tatsuzawa F, Yoshida R, Hiwasa-Tanase K, Wang N, Ariizumi T, Mizoguchi T*, Ezura H
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of subcellular localization of Pleckstrin-homology domain-cintaining protein, PH15.2016

    • Author(s)
      Arimoto S, Choi SW, Ebine K, Mizoguchi T*, Nakano A, Ueda T, Ito E
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diverse floral responses to day length among tomato species and difference within the regulatory mechanisms.2016

    • Author(s)
      Hayama R, Hoshikawa K, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Density effects on late and early flowering mutants of Arabidopsis thaliana under continuous light.2016

    • Author(s)
      Takahashi K, Yamamoto K, Hara M, Miyata K, Hayama R, Ito E, Choi SW, Mizoguchi T*
    • Organizer
      JSOL 2016, The 13th Japan Solanaceae Consortium
    • Place of Presentation
      国際基督教大学(東京都三鷹市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Density effects on flowering time of Arabidopsis thaliana2016

    • Author(s)
      Takahashi K, Yamamoto K, Hara M, Suzuki K, Miyata K, *Mizoguchi T(*発表者)
    • Organizer
      TJASSST 2015, TUNISIA-JAPAN Symposium on Science, Society and Technology
    • Place of Presentation
      University of Tsukuba, Tsukuba, Japan
    • Year and Date
      2016-02-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inversion of photoperiodic response by clock mutations in Arabidopsis thaliana2016

    • Author(s)
      Hara M, Natsui Y, *Kuroki K, Takahashi K, Miyata K, Mizoguchi T(*発表者)
    • Organizer
      TJASSST 2015, TUNISIA-JAPAN Symposium on Science, Society and Technology
    • Place of Presentation
      University of Tsukuba, Tsukuba, Japan
    • Year and Date
      2016-02-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 概日時計遺伝子変異による光周期応答性の改変:溝口剛、原美由紀、堂上久美子、荒川恵海、髙橋慶2014

    • Author(s)
      溝口剛、原美由紀、堂上久美子、荒川恵海、髙橋慶
    • Organizer
      第32回日本植物細胞分子生物学会
    • Place of Presentation
      岩手県民情報交流センター(アイーナ)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナの花成制御に及ぼす密度効果2014

    • Author(s)
      山本喜和子、髙橋慶、原美由紀、溝口剛
    • Organizer
      第32回日本植物細胞分子生物学会
    • Place of Presentation
      岩手県民情報交流センター(アイーナ)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Photoperiodic compensation: a mechanism underlying regulation of chlorophyll amount by circadian clock in Arabidopsis thaliana2013

    • Author(s)
      Mizoguchi, T, Miyata, K
    • Organizer
      24th International Conference on Arabidopsis Research (ICAR 2013)
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2013 Research-status Report
  • [Presentation] 光周期応答性の反転に関する分子遺伝学的研究

    • Author(s)
      溝口剛
    • Organizer
      日本時間生物学会、シンポジウム「光合成生物における時間生物学の研究手法を総括し学術の発展を探る」
    • Place of Presentation
      大阪府、近畿大学
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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