Studies on molecular mechanism underlying photoperiodic compensation

• Project/Area Number: 25440141
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: International Christian University
• Principal Investigator: MIZOGUCHI Tsuyoshi 国際基督教大学, 教養学部, 教授 (70281623)
• Co-Investigator(Kenkyū-buntansha): 楢本 悟史 東北大学, 生命科学研究科, 助教 (30612022)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 光合成 / 葉緑体 / 概日時計 / LHY / CCA1 / シロイヌナズナ / 光周性 / 既日時計 / クロロフィル / 補償性

Outline of Final Research Achievements

Circadian clock ensures consistency in antenna size under different photoperiod conditions. We named this novel clock function “photoperiodic compensation.” Double loss of function of LHY and CCA1 (lhy;cca1) resulted in changes in the total chlorophyll (Chl) level and Chl a/b ratio depending on the photoperiod. Under continuous light conditions (LL), the total Chl level increased significantly and the Chl a/b ratio decreased in lhy;cca1 compared with those in the wild type (WT). Increased stacked grana thylakoid membranes in chloroplasts were also observed in lhy;cca1 under LL. LIGHT HARVESTING COMPLEX II, which is an antenna protein involved in photosynthesis, accumulated to high levels in lhy;cca1 under LL compared with the WT.

Research Products

• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Journal Article] Density effects on late flowering mutants of <i>Arabidopsis thaliana</i> under continuous light (2016)
  • Author(s): Yamamoto K, Takahashi K, Hara M, Miyata K, Hayama R, Mizoguchi T*
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 323-331
  • DOI: 10.5511/plantbiotechnology.16.0622a
  • NAID: 130005281093
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Density effects on semi-dwarf and early flowering mutants of <i>Arabidopsis thaliana</i> under continuous light (2016)
  • Author(s): Takahashi K, Hara M, Miyata K, Hayama R, Mizoguchi T*
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 333-339
  • DOI: 10.5511/plantbiotechnology.16.0912a
  • NAID: 130005281091
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] A loss-of-function mutation in the <i>DWARF4</i>/<i>PETANKO5</i> gene enhances the late-flowering and semi-dwarf phenotypes of the <i>Arabidopsis</i> clock mutant <i>lhy-12;cca1-101</i> under continuous light without affecting <i>FLC</i> expression (2016)
  • Author(s): Suzuki S, Miyata K, Hara M, Niinuma K, Tsukaya H, Takase M, Hayama R, and Mizoguchi T
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 315-321
  • DOI: 10.5511/plantbiotechnology.16.0601a
  • NAID: 130005281084
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Environmental responses of plants: Biological interactions in the homogenous population or community (mixed populations) (2016)
  • Author(s): Mizoguchi T*, Funabashi M.
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 211-212
  • DOI: 10.5511/plantbiotechnology.16.0000p
  • NAID: 130005281055
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] The <i>FT-like</i> gene <i>PehFT</i> in petunia responds to photoperiod and light quality but is not the main gene promoting light quality-associated flowering (2016)
  • Author(s): Tsukamoto A, Hirai T, Chin DP, Mii M, Mizoguchi T*, Mizuta D, Yoshida H, Olsen J, Ezura H, Fukuda N.
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 297-307
  • DOI: 10.5511/plantbiotechnology.16.0620a
  • NAID: 130005281092
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] EMS mutagenesis and characterization of <i>Brassica rapa</i> mutants (2014)
  • Author(s): Mizuno, Y., Okamoto, S., Hara, M., Mizoguchi, T.
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 2 Pages: 185-190
  • DOI: 10.5511/plantbiotechnology.14.0109b
  • NAID: 130004435283
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] はじめに：GarnerとAllardが100年前に抱いた疑問, 特集：植物の概日時計と光周性花成応答 (2014)
  • Author(s): 溝口剛
  • Journal Title: 植物の生長調節 Regulation of Plant Growth & Development Volume: 49 Pages: 25-26
  • Peer Reviewed
• [Journal Article] 長日植物シロイヌナズナの概日時計遺伝子変異が及ぼす光周性花成応答への影響, 特集：植物の概日時計と光周性花成応答 (2014)
  • Author(s): 溝口剛
  • Journal Title: 植物の生長調節 Regulation of Plant Growth & Development Volume: 49 Pages: 27-33
  • Peer Reviewed
• [Journal Article] Increase in vascular pattern complexity caused by mutations in <i>LHY</i> and <i>CCA1</i> in <i>Arabidopsis thaliana</i> under continuous light (2014)
  • Author(s): Aihara, K, Naramoto, S, Hara, M, Mizoguchi, T
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 1 Pages: 43-47
  • DOI: 10.5511/plantbiotechnology.13.1015a
  • NAID: 130003391361
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Late flowering phenotype under ultra-short photoperiod (USP) in Arabidopsis thaliana (2014)
  • Author(s): Sugiyama, H, Natsui, Y, Hara, M, Miyata, K, Nefissi, R, Mizoguchi, T
  • Journal Title: Plant Biotechnology Volume: 31 Pages: 29-34
  • NAID: 130003391362
  • Peer Reviewed
• [Journal Article] <i>CO-EXPRESSED WITH CLOCK GENES LHY AND CCA1 1</i> (<i>CEC1</i>) is regulated by LHY and CCA1 and plays a key role in phase setting of <i>GI</i> in <i>Arabidopsis thaliana</i> (2014)
  • Author(s): Hara, M, Kamada, H, Mizoguchi, T
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 1 Pages: 35-41
  • DOI: 10.5511/plantbiotechnology.13.1108a
  • NAID: 130003391363
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Presentation] Circadian rhythm gating of transcription factors implicated in salinity stress in Solanum lycopersicum (2016)
  • Author(s): Coyne K, Hayama R, Mullen DM, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Gibberellic acid and PTA7 may control flowering time via a common genetic pathway in Arabidopsis thaliana. (2016)
  • Author(s): Miyata K, Kobayashi M, Murayama Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Natural variation in the photoperiodic response of elf3;gi between Landsberg erecta and Columbia ecotypes in Arabidopsis thaliana. (2016)
  • Author(s): Murayama Y, Kuroki K, Sakamoto S, Fujiwara S, Hara M, Natsui Y, Takahashi K, Miyata K, Hayama R, Ito E, Choi SW, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Effect of night break on flowering time and FT expression in elf3;gi, an SDP-type mutant of Arabidopsis thaliana. (2016)
  • Author(s): Nakai H, Hara M, Takahashi K, Ariga H, Murayama Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Inversion of photoperiodic response by double loss-of-function mutations in EARLY FLOWERING 3 and GIGANTEA in Arabidopsis thaliana. (2016)
  • Author(s): Hara M, Natsui Y, Murayama Y, Kuroki K, Takahashi K, Miyata K, Chiba Y, Hayama R, Ito E, Choi SW, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Involvement of SELF PRUNING 5G in the floral and sympodial regulation in a day-neutral plant tomato. (2016)
  • Author(s): Kato K, Oikawa J, Ishigami Y, Shimoyama S, Shiba T, Tatsuzawa F, Yoshida R, Hiwasa-Tanase K, Wang N, Ariizumi T, Mizoguchi T*, Ezura H
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Analysis of subcellular localization of Pleckstrin-homology domain-cintaining protein, PH15. (2016)
  • Author(s): Arimoto S, Choi SW, Ebine K, Mizoguchi T*, Nakano A, Ueda T, Ito E
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Diverse floral responses to day length among tomato species and difference within the regulatory mechanisms. (2016)
  • Author(s): Hayama R, Hoshikawa K, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Density effects on late and early flowering mutants of Arabidopsis thaliana under continuous light. (2016)
  • Author(s): Takahashi K, Yamamoto K, Hara M, Miyata K, Hayama R, Ito E, Choi SW, Mizoguchi T*
  • Organizer: JSOL 2016, The 13th Japan Solanaceae Consortium
  • Place of Presentation: 国際基督教大学（東京都三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Density effects on flowering time of Arabidopsis thaliana (2016)
  • Author(s): Takahashi K, Yamamoto K, Hara M, Suzuki K, Miyata K, ＊Mizoguchi T（＊発表者）
  • Organizer: TJASSST 2015, TUNISIA-JAPAN Symposium on Science, Society and Technology
  • Place of Presentation: University of Tsukuba, Tsukuba, Japan
  • Year and Date: 2016-02-23
  • Int'l Joint Research
• [Presentation] Inversion of photoperiodic response by clock mutations in Arabidopsis thaliana (2016)
  • Author(s): Hara M, Natsui Y, ＊Kuroki K, Takahashi K, Miyata K, Mizoguchi T（＊発表者）
  • Organizer: TJASSST 2015, TUNISIA-JAPAN Symposium on Science, Society and Technology
  • Place of Presentation: University of Tsukuba, Tsukuba, Japan
  • Year and Date: 2016-02-23
  • Int'l Joint Research
• [Presentation] 概日時計遺伝子変異による光周期応答性の改変：溝口剛、原美由紀、堂上久美子、荒川恵海、髙橋慶 (2014)
  • Author(s): 溝口剛、原美由紀、堂上久美子、荒川恵海、髙橋慶
  • Organizer: 第32回日本植物細胞分子生物学会
  • Place of Presentation: 岩手県民情報交流センター（アイーナ）
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] シロイヌナズナの花成制御に及ぼす密度効果 (2014)
  • Author(s): 山本喜和子、髙橋慶、原美由紀、溝口剛
  • Organizer: 第32回日本植物細胞分子生物学会
  • Place of Presentation: 岩手県民情報交流センター（アイーナ）
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Photoperiodic compensation: a mechanism underlying regulation of chlorophyll amount by circadian clock in Arabidopsis thaliana (2013)
  • Author(s): Mizoguchi, T, Miyata, K
  • Organizer: 24th International Conference on Arabidopsis Research (ICAR 2013)
  • Place of Presentation: Sydney, Australia
• [Presentation] 光周期応答性の反転に関する分子遺伝学的研究
  • Author(s): 溝口剛
  • Organizer: 日本時間生物学会、シンポジウム「光合成生物における時間生物学の研究手法を総括し学術の発展を探る」
  • Place of Presentation: 大阪府、近畿大学
  • Invited