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Functional analysis of a gene that is involved in seed aleurone and epidermis development

Research Project

Project/Area Number 25440142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionMeiji University

Principal Investigator

Kawakami Naoto  明治大学, 農学部, 教授 (10211179)

Co-Investigator(Renkei-kenkyūsha) Toyooka Kiminori  理化学研究所, 環境資源科学研究センター, 上級研究員 (10360596)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords種子 / 胚乳 / 糊粉層 / 発芽 / 小胞輸送 / 細胞分化 / 植物生理学 / 形態形成 / 表皮 / クラスリン被覆小胞
Outline of Final Research Achievements

Outermost cell layer of endosperm, aleurone, works for mobilization of storage materials in cereal grains. In contrast, aleurone works mainly as a barrier of germination in dicot seeds. In this study, we found TRG2A gene as an important factor for aleurone layer development and/or maintenance by using Arabiodpsis mutant that lost all the endosperm cells during seed development. TRG2A protein was found to be localized in cytoplasm and interact with adaptin subunits. We found that CHMP1 is also involved in aleurone development in Arabidopsis. Our findings indicate that vesicle transport system has critical role on aleurone development.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression of SOMNUS
and Other High-Temperature-Inducible Genes
in Imbibed Seeds in Arabidopsis2013

    • Author(s)
      Soohwan Lim, Jeongmoo Park, Nayoung Lee, Jinkil Jeong, Shigeo Toh, Asuka Watanabe, Junghyun Kim, Hyojin Kang, Dong Hwan Kim, Naoto Kawakami, and Giltsu Choi
    • Journal Title

      The Plant Cell

      Volume: 25 Issue: 12 Pages: 4863-4878

    • DOI

      10.1105/tpc.113.118604

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Functional analysis of TRG2A gene which is involved in the development of endosperm aleurone in Arabidopsis seed2016

    • Author(s)
      Yuki Uchida, Kazutaka Akahori, Reo Sugiyama, Yukinori Yoshino, Michio Sato, Kiminori Toyooka, Naoto Kawakami
    • Organizer
      5th Workshop on the Molecular Aspects of Seed Dormancy and Germination
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2016-05-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 種子の糊粉層の形成・維持に関わるシロイヌナズナのTRG2Aタンパク質の解析2016

    • Author(s)
      赤堀 一貴,杉山 礼央,内田 悠稀,吉野 幸則,佐藤 道夫,豊岡 公徳,川上 直人
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] アダプチン結合領域を持つTRG2タンパク質の機能解析2015

    • Author(s)
      杉山礼央、吉野幸則、赤堀一貴、内田悠稀、川上直人
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胚乳最外層の消失と植物体の形態異常をもたらすシロイヌナズナtrg2突然変異の解析2015

    • Author(s)
      吉野幸則、赤堀一貴、杉山礼央、鴨志田葵、内田悠輝、田中新太、五味渕苑子、佐藤道夫、川上直人
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of Arabidopsis thermoinhibition resistant germination 2 that has defect in aleurone layer and epidermis2014

    • Author(s)
      Yukinori Yoshino, Arata Tanaka, Sonoko Gomibuchi, Kazutaka Akahori, Reo Sugiyama, Aoi Kamoshita and Naoto Kawakami
    • Organizer
      XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
    • Place of Presentation
      東京
    • Year and Date
      2014-10-27 – 2014-10-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 種子の糊粉層形成に異常を持つシロイヌナズナの突然変異体trg2の解析2014

    • Author(s)
      赤堀一貴、吉野幸則、五味渕苑子、杉山礼央、鴨志田葵、川上直人
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      川崎
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of TRG2 as a novel gene that is involved in development and/or maintenance of aleurone layer cells of Arabidopsis seeds2013

    • Author(s)
      Sonoko Gomibuchi, Kazutaka Akahori, Arata Tanaka and Naoto Kawakami
    • Organizer
      4th Workshop on the Molecular Aspects of Seed Dormancy and Germination
    • Place of Presentation
      パリ
    • Related Report
      2013 Research-status Report
  • [Presentation] 種子の糊粉層形成に異常を持つシロイヌナズナの突然変異 体trg2 の解析

    • Author(s)
      吉野幸則、五味渕苑子、赤堀一貴、笹本真語、田中新太、山下結、川上直人
    • Organizer
      第34回 種子生理生化学研究会年会
    • Place of Presentation
      箱根
    • Related Report
      2013 Research-status Report
  • [Presentation] 種子糊粉層の消失をもたらすシロイヌナズナtrg2突然変異遺伝子の解析

    • Author(s)
      吉野 幸則,五味渕 苑子,赤堀 一貴,杉山 礼央,鴨志田 葵,川上 直人
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Remarks] 教員データベース

    • URL

      http://gyoseki1.mind.meiji.ac.jp/mjuhp/KgApp?kyoinId=ymiygyymggy

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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