Molecular mechanisms underlining the formation of endoplasmic reticulum (ER) bodies in plants

• Project/Area Number: 25440146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University (2014-2015); National Institute for Basic Biology (2013)
• Principal Investigator: Kenji Yamada 京都大学, 理学(系)研究科(研究院), 研究員 (00360339)
• Co-Investigator(Renkei-kenkyūsha): NISHIMURA MIKIO 基礎生物学研究所, 高次細胞機構研究部門, 教授 (80093061) ; NISHIMURA IKUKO 京都大学, 理学研究科, 教授 (00241232)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 小胞体 / ERボディ / シロイヌナズナ / βグルコシダーゼ / 食害 / 傷害 / 膜タンパク質 / グルコシノレート / ダンゴムシ / ＥＲボディ / 植物・微生物相互作用 / オルガネラ / 病虫害抵抗性 / ミロシナーゼ / 膜輸送体 / 金属イオン

Outline of Final Research Achievements

ER bodies are organelles derived from the endoplasmic reticulum in plants of the Brassicales order, including Arabidopsis thaliana. A unique protein NAI2 and a β-glucosidase PYK10 are accumulated in ER bodies in A. thaliana. In this study, we investigated the role of these proteins and obtained new information for the ER body formation and function. We found that NAI2 and PYK10 were necessary and sufficient for the ER body formation. PYK10 was a myrosinase to hydrolyze glucosinolates, and it prevented the feeding damage by woodlice. These findings suggest that ER bodies are responsible for the protection against pests and pathogens.

Research Products

• [Journal Article] Hexose oxidase-mediated hydrogen peroxide as a mechanism for the antibacterial activity in the red seaweed Ptilophora subcostata. (2015)
  • Author(s): Ogasawara K, Yamada K, Hatsugai N, Imada C, Nishimura M
  • Journal Title: PLoS ONE Volume: 56 Issue: 2 Pages: e0149084-e0149084
  • DOI: 10.1371/journal.pone.0149084
  • NAID: 120005763394
  • Peer Reviewed / Open Access
• [Journal Article] Dynamics of the light-dependent transition of plant peroxisomes. (2015)
  • Author(s): Goto-Yamada, S., Mano, S., Yamada, K., Oikawa, K., Hosokawa, Y., Hara-Nishimura, I., and Nishimura, M.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 7 Pages: 1252-1263
  • DOI: 10.1093/pcp/pcv081
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Vacuolar processing enzyme in plant programmed cell death (2015)
  • Author(s): Noriyuki Hatsugai, Kenji Yamada, Shino Goto-Yamada and Ikuko Hara-Nishimura
  • Journal Title: Front Plant Sci Volume: 6 Pages: 1-11
  • DOI: 10.3389/fpls.2015.00234
  • NAID: 120005670913
  • Description: 【掲載確定】
  • Peer Reviewed / Open Access
• [Journal Article] Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis (2015)
  • Author(s): Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa & Mikio Nishimura
  • Journal Title: Nature Plants Volume: 1 Issue: 4 Pages: 15035-15035
  • DOI: 10.1038/nplants.2015.35
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ER bodies in plants of the Brassicales order: Biogenesis and association with innate immunity. (2014)
  • Author(s): Nakano, RT., K. Yamada, P. Bednarek, M. Nishimura and I. Hara-Nishimura
  • Journal Title: Frontiers in Plant Science Volume: 5 Issue: 5 Pages: 1-17
  • DOI: 10.4161/auto.28529
  • NAID: 120005439544
• [Journal Article] ER bodies in plants of the Brassicales order: Biogenesis and association with innate immunity. (2014)
  • Author(s): Nakano RT, Yamada K, Bednarek P, Nishimura M, Hara-Nishimura I
  • Journal Title: Front. Plant Sci. Volume: 5 Pages: 73-73
  • DOI: 10.3389/fpls.2014.00073
  • NAID: 120005439544
  • Peer Reviewed
• [Journal Article] Plant autophagy is responsible for peroxisomal transition and plays an important role in the maintenance of peroxisomal quality. (2014)
  • Author(s): M. Shibata, K. Oikawa, K. Yoshimoto, S. Goto-Yamada, S. Mano, K. Yamada, M. Kondo, M. Hayashi, W. Sakamoto, Y. Ohsumi, M. Nishimura
  • Journal Title: Autophagy Volume: 10
• [Journal Article] Highly Oxidized Peroxisomes Are Selectively Degraded via Autophagy in Arabidopsis (2013)
  • Author(s): Shibata, M., Oikawa, K., Yoshimoto, K., Kondo., M., Mano, S., Yamada, K, Hayashi, M, Sakamoto, W., Ohsumi. Y., and Nishimura. M
  • Journal Title: The Plant Cell Volume: 25 Issue: 12 Pages: 4967-4983
  • DOI: 10.1105/tpc.113.116947
  • Peer Reviewed
• [Journal Article] Novel proteins interacting with peroxisomal protein receptor PEX7 in Arabidopsis thaliana. (2013)
  • Author(s): Cui S, Mano S, Yamada K, Hayashi M, Nishimura M.
  • Journal Title: Plant Signal Behav. Volume: 8 Issue: 10 Pages: e26829-e26829
  • DOI: 10.4161/psb.26829
  • Peer Reviewed
• [Journal Article] Proteomic analysis reveals the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEX7 (Peroxin 7) (2013)
  • Author(s): Soungkui Cui, Yoichiro Fukao, Shoji Mano, Kenji Yamada, Makoto Hayashi and Mikio Nishimura
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 8 Pages: 6014-6023
  • DOI: 10.1074/jbc.m112.438143
  • Peer Reviewed
• [Journal Article] Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. (2013)
  • Author(s): Yamada K et al.
  • Journal Title: Plant Physiol. Volume: 161(1) Issue: 1 Pages: 108-120
  • DOI: 10.1104/pp.112.207654
  • Peer Reviewed
• [Presentation] Endomembrane-mediated plant strategies against pathogens and herbivores. (2016)
  • Author(s): K. Yamada, I. Hara-Nishimura
  • Organizer: Kyoto-Bristol-Heidelberg Plant Sciences Workshop 2016
  • Place of Presentation: Kyoto University, Kyoto, Japan
  • Year and Date: 2016-03-02
  • Int'l Joint Research
• [Presentation] Mechanism of ER body formation and function in Arabidopsis thaliana. (2014)
  • Author(s): Yamada K
  • Organizer: The 5th NIBB-MPIZ-TLL Joint Symposium
  • Place of Presentation: Cologne, Germany
  • Year and Date: 2014-11-24 – 2014-11-26
• [Presentation] シロイヌナズナのERボディの形成と機能について (2014)
  • Author(s): 山田健志
  • Organizer: 第3回植物エンドメンブレンミーティング
  • Place of Presentation: 名古屋大学，愛知
  • Year and Date: 2014-09-29 – 2014-09-30
• [Presentation] ERボディの膜特異的に局在するMEBタンパク質の解析 (2014)
  • Author(s): 山田健志，永野惇，西村いくこ，西村幹夫
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学，富山