glycosyltransferases for plant specialized compounds based on the 3D structures

• Project/Area Number: 25440148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yonekura-Sakakibara Keiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (20360555)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 配糖化酵素 / フラボノイド / シロイヌナズナ / 結晶構造

Outline of Final Research Achievements

Glycosylation plays an important role in the huge chemical diversity of plant metabolites. However, the knowledge about high-order glycosylation is limited. Four crystal structures of a flavonoid high-order glycosyltransferase UGT79B1 were dissolved. Genes encoding novel flavonoid high-order glycosyltransferases from Arabidopsis and soybean were also identified. Based on these data, amino acid residues which play crucial roles in the binding of substrates and substrate inhibition were found and biochemical analyses using mutated UGT79B1 proteins were conducted.

Research Products

• [Journal Article] Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1→2)glucosyltransferase (2015)
  • Author(s): S. Di, F. Yan, F. R. Rodas,T. O Rodriguez, Y. Murai, T. Iwashina, S. Sugawara, T. Mori, R. Nakabayashi, K. Yonekura-Sakakibara, K. Saito, R. Takahashi
  • Journal Title: BMC Plant Biology Volume: 15 Issue: 1 Pages: 126-126
  • DOI: 10.1186/s12870-015-0504-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A flavonoid 3-O-glucoside: 2”-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana. (2014)
  • Author(s): Keiko Yonekura-Sakakibara, Ryo Nakabayashi, Satoko Sugawara, Takayuki Tohge, Takuya Ito, Misuzu Koyanagi, Mariko Kitajima, Hiromitsu Takayama, and Kazuki Saito
  • Journal Title: Plant J. Volume: 79 Issue: 5 Pages: 769-782
  • DOI: 10.1111/tpj.12580
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Linkage mapping, molecular cloning and functional analysis of soybean gene Fg2 encoding flavonol 3-O-glucoside (1 →6) rhamnosyltransferase (2013)
  • Author(s): Rodas FR, Rodriguez TO, Murai Y, Iwashina T, Sugawara S, Suzuki M, Nakabayashi R, Yonekura-Sakakibara K, Saito K, Kitajima J, Toda K, Takahashi R
  • Journal Title: Plant Molecular Biology Volume: 84 Issue: 3 Pages: 287-300
  • DOI: 10.1007/s11103-013-0133-1
  • Peer Reviewed
• [Journal Article] The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity (2013)
  • Author(s): Kazuki Saito, Keiko Yonekura-Sakakibara, Ryo Nakabayashi, Yasuhiro Higashi, Mami Yamazaki, Takayuki Tohge, Alisdair R. Fernie
  • Journal Title: Plant Physiol. Biochem. Volume: 72 Pages: 21-34
  • DOI: 10.1016/j.plaphy.2013.02.001
  • Peer Reviewed
• [Presentation] Flavonol 3-O-gal:2"-O-glucosyltransferase from petunia pollen (2016)
  • Author(s): Eva Knoch, Satoko Sugawara, Kazuki Saito, Keiko Yonekura-Sakakibara
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手市）
  • Year and Date: 2016-03-19
• [Presentation] Structure, function and diversity of plant glycosyltransferases (2016)
  • Author(s): Keiko Yonekura-Sakakibara
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Genetic and structural variety of glycosyltransferases bring chemical diversity of flavonoids (2015)
  • Author(s): Keiko Yonekura-Sakakibara, Kazuki Saito
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem)
  • Place of Presentation: Hilton Waikiki Beachhotel （Honolulu, Hawaii）
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Integrated ‘Omics’ analyses in a model plant, Arabidopsis thaliana (2015)
  • Author(s): Keiko Yonekura-Sakakibara, Kazuki Saito
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
  • Place of Presentation: ポートアイランド(神戸)
  • Year and Date: 2015-12-03
  • Invited
• [Presentation] トランスクリプトミクス・メタボロミクスを基盤としたフラボノイド代謝研究 (2015)
  • Author(s): 榊原圭子
  • Organizer: 地球環境・食糧・資源のための植物バイオ第160委員会 第11回研究会「植物天然物生合成研究の新しい展開」
  • Place of Presentation: サントリーワールドリサーチセンター（京都府相楽郡）
  • Year and Date: 2015-10-23
  • Invited
• [Presentation] シロイヌナズナ花粉特異的フラボノールの高次配糖化酵素UGT79B6の局在とその生理的役割 (2015)
  • Author(s): 榊原圭子、菅原聡子、斉藤和季
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナにおけるフラボノイド多様性の包括的理解 (2014)
  • Author(s): 榊原圭子
  • Organizer: 第270回生存圏シンポジウム/第3回植物二次代謝フロンティア研究会
  • Place of Presentation: 小田原お堀端コンベンションホール（神奈川県）
  • Year and Date: 2014-11-23
  • Invited
• [Presentation] A flavonoid glycosyltransferase, UGT79B6, determines the pollen-specific flavonol structures in Arabidopsis thaliana (2014)
  • Author(s): K. Yonekura-Sakakibara
  • Organizer: XII France-Japan Workshop on Plant Science 2014
  • Place of Presentation: 東京大学（東京都）
  • Year and Date: 2014-10-27 – 2014-10-29
  • Invited
• [Presentation] Functional identification of a glycosyltransferase involved in pollen-specific flavonol biosynthesis in Arabidopsis thaliana (2014)
  • Author(s): K. Yonekura-Sakakibara, R. Nakabayashi, S. Sugawara, T. Tohge, T. Ito, M. Koyanagi, M. Kitajima, H. Takayama, K. Saito
  • Organizer: XXVII International Conference on Polyphenols & Tannin conference
  • Place of Presentation: 名古屋大学（愛知県）
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Pollen-specific flavonol biosynthesis in Arabidopsis thaliana: identification of the key enzyme UGT33 and the tailored flavonol structures (2014)
  • Author(s): K. Yonekura-Sakakibara, R. Nakabayashi, S. Sugawara, T. Tohge, T. Ito, M. Koyanagi, M. Kitajima, H. Takayama, K. Saito
  • Organizer: 25th International Cnference on Arabidopsis Research
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] シロイヌナズナ花粉特異的フラボノールの高次配糖化酵素遺伝子の機能同定とその局在性 (2014)
  • Author(s): 榊原圭子、中林亮、菅原聡子、峠隆之、伊藤卓也、小柳美寿々、北島満里子、高山廣光、斉藤和季
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学（富山県）
• [Presentation] Functional identification of a key glycosyltransferase, UGT33, determining the pollen-specific flavonol structures in Arabidopsis (2014)
  • Author(s): Keiko Yonekura-Sakakibara
  • Organizer: Plant Gene Discovery & “Omics” Technologies
  • Place of Presentation: Vienna, Austria
• [Presentation] 花粉特異的なシロイヌナズナ・フラボノール高次配糖化酵素の機能同定 (2013)
  • Author(s): 榊原圭子、菅原聡子、中林亮、小柳美寿々、峠隆之、鈴木実、伊藤卓也、北島満里子、高山廣光、斉藤和季
  • Organizer: 第31回日本植物細胞分子生物学会大会シンポジウム
  • Place of Presentation: 北海道大学（北海道）
• [Book] Resent Advances in Polyphenol Research vol.4 (2014)
  • Author(s): K. Yonekura-Sakakibara, K. Saito
  • Total Pages: 464
  • Publisher: Wiley-Blackwell
• [Book] Resent Advances in Polyphenol Research (2014)
  • Author(s): K. Yonekura-Sakakibara, K. Saito
  • Publisher: Wiley-Blackwell