glycosyltransferases for plant specialized compounds based on the 3D structures
Project/Area Number |
25440148
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 配糖化酵素 / フラボノイド / シロイヌナズナ / 結晶構造 |
Outline of Final Research Achievements |
Glycosylation plays an important role in the huge chemical diversity of plant metabolites. However, the knowledge about high-order glycosylation is limited. Four crystal structures of a flavonoid high-order glycosyltransferase UGT79B1 were dissolved. Genes encoding novel flavonoid high-order glycosyltransferases from Arabidopsis and soybean were also identified. Based on these data, amino acid residues which play crucial roles in the binding of substrates and substrate inhibition were found and biochemical analyses using mutated UGT79B1 proteins were conducted.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1→2)glucosyltransferase2015
Author(s)
S. Di, F. Yan, F. R. Rodas,T. O Rodriguez, Y. Murai, T. Iwashina, S. Sugawara, T. Mori, R. Nakabayashi, K. Yonekura-Sakakibara, K. Saito, R. Takahashi
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Journal Title
BMC Plant Biology
Volume: 15
Issue: 1
Pages: 126-126
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Linkage mapping, molecular cloning and functional analysis of soybean gene Fg2 encoding flavonol 3-O-glucoside (1 →6) rhamnosyltransferase2013
Author(s)
Rodas FR, Rodriguez TO, Murai Y, Iwashina T, Sugawara S, Suzuki M, Nakabayashi R, Yonekura-Sakakibara K, Saito K, Kitajima J, Toda K, Takahashi R
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Journal Title
Plant Molecular Biology
Volume: 84
Issue: 3
Pages: 287-300
DOI
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Peer Reviewed
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[Presentation] Pollen-specific flavonol biosynthesis in Arabidopsis thaliana: identification of the key enzyme UGT33 and the tailored flavonol structures2014
Author(s)
K. Yonekura-Sakakibara, R. Nakabayashi, S. Sugawara, T. Tohge, T. Ito, M. Koyanagi, M. Kitajima, H. Takayama, K. Saito
Organizer
25th International Cnference on Arabidopsis Research
Place of Presentation
University of British Columbia, Vancouver, Canada
Year and Date
2014-07-28 – 2014-08-01
Related Report
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