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Regulatory mechanism of starfish reproduction by relaxin-like gonad-simulating hormone (GSS)

Research Project

Project/Area Number 25440151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionTokyo Gakugei University

Principal Investigator

MITA MASATOSHI  東京学芸大学, 教育学部, 教授 (50190674)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生殖腺刺激ホルモン / リラキシン / ペプチドホルモン / オーソログ / シグナル情報伝達機構 / ヒトデ / 生理活性 / 比較内分泌 / 卵成熟誘起ホルモン / 生殖生物学
Outline of Final Research Achievements

The potential of ovarian follicle cells to response to GSS was brought by de novo synthesis of Gαs-proteins. On the basis of homology search using conservative nucleotide sequences of relaxin receptor in vertebrates, cDNA for a candidate of GSS receptor was obtained from ovarian follicle cells of the starfish Asterina pectinifera. This study also showed that 1-methyladenine was produced from S-adenosyl-[methyl-13C]-methionine, when follicle cells had been preincubated in [methyl-13C]-methionine. Furthermore, chemical structures of GSS were identified in ten species of starfish. Three kinds of RGP orthologs were found in the class Asteroidea. Based on their cysteine motifs, starfish GSS molecules are classified as member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily and, more precisely, they belong to a relaxin-like peptide family. Therefore, gonadotropin in starfish is designated as relaxin-like gonad-stimulating peptide (RGP).

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (3 results) Journal Article (13 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 13 results,  Acknowledgement Compliant: 8 results,  Open Access: 4 results) Presentation (19 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results) Book (2 results) Remarks (6 results)

  • [Int'l Joint Research] Queen Mary University of London(英国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] National Taiwan Ocean University(Taiwan)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Hawaii Institute of Marine Biology(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera2016

    • Author(s)
      Haraguchi S, Ikeda N, Abe M, Tsutsui K, Mita M
    • Journal Title

      Gen Comp Endocrinol

      Volume: 227 Pages: 115-9

    • DOI

      10.1016/j.ygcen.2015.06.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A relaxin-like gonad-stimulating peptide from the starfish aphelasterias japonica2016

    • Author(s)
      Masatoshi Mita and Hidetoshi Katayama
    • Journal Title

      General and Comparative Endocrinology

      Volume: 229 Pages: 56

    • DOI

      10.1016/j.ygcen.2016.02.025

    • URL

      https://pure.teikyo.jp/en/publications/68296374-732f-401d-99c2-33960bb43a4b

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Starfish gonadotropic hormone: Relaxin-like gonad-stimulating peptides2016

    • Author(s)
      Masatoshi Mita
    • Journal Title

      General and Comparative Endocrinology

      Volume: 230-231 Pages: 166-169

    • DOI

      10.1016/j.ygcen.2016.04.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Relaxin-like gonad-stimulating peptide is highly conserved in starfish Asterina pectinifera2015

    • Author(s)
      Narumi Ikeda, Haruka Uzawa, Misaki Daiya, Shogo Haraguchi, Kazuyoshi Tsutsui and Masatoshi Mita
    • Journal Title

      Invertebrates Reproduction & Development

      Volume: 59 Issue: 4 Pages: 224-229

    • DOI

      10.1080/07924259.2015.1091388

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci2015

    • Author(s)
      Masatoshi Mita, Narumi Ikeda, Shogo Haraguchi, Kazuyoshi Tsutsui, Yoshikatsu Nakano and Masaru Nakamura
    • Journal Title

      Invertebrates Reproduction & Development

      Volume: 59 Issue: 4 Pages: 212-217

    • DOI

      10.1080/07924259.2015.1086828

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon2015

    • Author(s)
      Haraguchi S, Yamamoto Y, Suzuki Y, Hyung Chang J, Koyama T, Sato M, Mita M, Ueda H, Tsutsui K
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 12546-12546

    • DOI

      10.1038/srep12546

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A new relaxin-like gonad-stimulating peptide identified in the starfish Asterias amurensis2015

    • Author(s)
      Mita M, Daiya M, Haraguchi S, Tsutsui K, Nagahama Y
    • Journal Title

      Gen Comp Endocrinol

      Volume: 222 Pages: 144-9

    • DOI

      10.1016/j.ygcen.2015.07.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Relaxin-like gonad-stimulating peptide is a highly conserved peptide in starfish Asterina pectinifera2015

    • Author(s)
      Narumi Ikeda, Haruka Uzawa, Misaki Daiya, Shogo Haraguchi, Kazuyoshi Tsutsui and Masatoshi Mita
    • Journal Title

      Invertebrate Reproduction and Development

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterine pectinifera2015

    • Author(s)
      Shogo Haraguchia, Narumi Ikeda, Michiko Abe, Kazuyoshi Tsutsui, Masatoshi Mita
    • Journal Title

      General and Comparative Endocrinology

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of Gas-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells.2014

    • Author(s)
      M. Mita, S. Haraguchi, M. Watanabe, Y. Takeshige, K. Yamamoto and K. Tsutsui
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 205 Pages: 80-87

    • DOI

      10.1016/j.ygcen.2014.06.003

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Release of relaxin-like gonad-stimulating substance from starfish radial nerves by ionomycin.2013

    • Author(s)
      Masatoshi Mita
    • Journal Title

      Zoological Science

      Volume: 30 Issue: 7 Pages: 602-606

    • DOI

      10.2108/zsj.30.602

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relaxin-like gonad-stimulating substance in an echinoderm, the starfish: A novel relaxin system in reproduction of invertebrates.2013

    • Author(s)
      Masatoshi Mita
    • Journal Title

      General and Comparative Endocrinology

      Volume: 181 Pages: 241-245

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contribution of de novo synthesis of Gαs-proteins to 1-methyladenine production in starfish ovarian follicle cells stimulated by relaxin-like gonad-stimulating substance.2013

    • Author(s)
      Masatoshi Mita, Shogo Haraguchi, Haruka Uzawa, Kazuyoshi Tsutsui
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 440 Pages: 798-801

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Relaxin-like gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci2015

    • Author(s)
      Masatoshi Mita, Narumi Ikeda, Shogo Haraguchi, Kazuyoshi Tsutsui, Yoshikatsu Nakano, Masaru Nakamura
    • Organizer
      CompBiol2015 第40回日本比較内分泌学会・第37回日本比較生理生化学会 合同大会
    • Place of Presentation
      広島アステールプラザ, 広島
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chemical synthesis of relaxin-like gonad stimulating peptide (RGP) and its PEG derivatives responsible for gonadotropic hormone of the starfish Asterina pectinifera2015

    • Author(s)
      Hidekazu Katayama, Masatoshi Mita
    • Organizer
      CompBiol2015 第40回日本比較内分泌学会・第37回日本比較生理生化学会 合同大会
    • Place of Presentation
      広島アステールプラザ, 広島
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒトデの生殖腺刺激ホルモンであるリラキシン様生殖腺刺激ペプチド (RGP) の遺伝子解析およびその発現2015

    • Author(s)
      原口省吾, 池田成美, 阿部倫子, 筒井和義, 三田雅敏
    • Organizer
      BMB2015 第38回日本分子生物学会・第88回日本生化学会 合同大会
    • Place of Presentation
      神戸国際展示場, 神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] リラキシン様生殖腺刺激ペプチド(RGP)の受容体の探索2015

    • Author(s)
      原口省吾, 鵜澤晴香, 山本和俊, 筒井和義, 三田雅敏
    • Organizer
      第6回ペプチド・ホルモン研究会
    • Place of Presentation
      金沢大学環日本海域環境センター, 能登, 石川
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Relaxin-like gonad-stimulating peptide in an echinoderm, the starfish: A novel relaxin system in reproduction of invertebrates2015

    • Author(s)
      Masatoshi Mita
    • Organizer
      7th International Conference “Relaxin and Related Peptides”
    • Place of Presentation
      Damai Beach Resort, Malaysia
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マヒトデのリラキシン様生殖腺刺激ホルモン2015

    • Author(s)
      三田雅敏, 大家美咲, 原口省吾, 筒井和義, 長濱嘉孝
    • Organizer
      第86回日本動物学会大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター, 新潟
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Starfish gonadotropin, relaxin-like gonad-stimulating peptide: the gene and its expression2015

    • Author(s)
      Shogo Haraguchi, Narumi Ikeda, Michio Abe, Kazuyoshi Tsutsui and Masatoshi Mita
    • Organizer
      3rd Biennial Conference of The North American Society for Comparative Endocrinology
    • Place of Presentation
      University of Ottawa, Ottawa, Canada
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サンゴを食害するオニヒトデの生殖制御に関する技術開発2015

    • Author(s)
      三田雅敏
    • Organizer
      平成26年度・亜熱帯性動植物に関する調査研究・技術開発研究会
    • Place of Presentation
      沖縄美ら島財団総合研究センター、沖縄県本部
    • Year and Date
      2015-02-21
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Analysis of cDNA sequences of relaxin-like gonad-stimulating substance in starfish Asterina pectinifera from Japanese waters.2014

    • Author(s)
      Narumi Ikeda, Shogo Haraguchi, Kazuyoshi Tsutsui, Masatoshi Mita
    • Organizer
      The 39th Annual Meeting of the Japan Society for Comparative Endocrinology/ 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      岡崎コンファレンスセンター、岡崎市
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒトデ生殖腺刺激ホルモンGSSの作用機構に対するGαsタンパク質の影響2014

    • Author(s)
      三田雅敏, 原口省吾, 山本和俊, 筒井和義
    • Organizer
      第85回日本動物学会
    • Place of Presentation
      東北大学川内北キャンパス、仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Comparative analysis of relaxin-like gonad-stimulating substance in several starfish species.2014

    • Author(s)
      Masatoshi Mita, Shogo Haraguchi, Dean C. Semmens, Maurice R. Elphick, Kazuyoshi Tsutsui
    • Organizer
      The 27th Conference of European Comparative Endocrinologists
    • Place of Presentation
      Universite de Renne 1, Rennes, France
    • Year and Date
      2014-08-25 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Regulatory mechanism of relaxin-like gonad-stimulating substance in reproduction of starfish

    • Author(s)
      Masatoshi Mita
    • Organizer
      Research Meeting in Kashiwa Campus "Diverse Strategies for Adaptation in Marine Organisms"
    • Place of Presentation
      東京大学大気海洋研究所柏キャンパス
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Involvement of Gs-proteins in the action of gonad-stimulating substance on starfish ovarian follicle cells

    • Author(s)
      Mita, M., Haraguchi, S., Yamamoto, K., and Tsutsui, K.
    • Organizer
      The 17th International Congress of Comparative Endocrinology
    • Place of Presentation
      Universitat de Barcelona (Barcelona, Spain)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] An increase in expression of Gsα-protein in ovarian follicle cells following oogenesis in starfish

    • Author(s)
      Mita, M., Haraguchi, S., Watanabe, M., Takeshige, Y., and Tsutsui, K.
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜 (横浜)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトデ卵濾胞細胞の1-メチルアデニン生産に対するCa2+欠如海水処理の影

    • Author(s)
      渡辺美秀, 山本和俊, 三田雅敏
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学津島キャンパス (岡山)
    • Related Report
      2013 Research-status Report
  • [Presentation] 解離したイトマキヒトデ卵濾胞細胞は生殖腺刺激ホルモン(GSS)に対する応答を消失する

    • Author(s)
      渡邉美秀, 原口省吾, 山本和俊, 筒井和義, 三田雅敏
    • Organizer
      第38回日本比較内分泌学会大会・第40回日本神経内分泌学会学術集会合同大会
    • Place of Presentation
      宮崎市民プラザ (宮崎)
    • Related Report
      2013 Research-status Report
  • [Presentation] グルタミン酸によるイトマキヒトデ放卵抑制作用について

    • Author(s)
      三田雅敏, 原口省吾, 筒井和義, 中村 將
    • Organizer
      第38回日本比較内分泌学会大会・第40回日本神経内分泌学会学術集会合同大会
    • Place of Presentation
      宮崎市民プラザ (宮崎)
    • Related Report
      2013 Research-status Report
  • [Presentation] イトマキヒトデ卵濾胞細胞の生殖腺刺激ホルモンに対するシグナル情報伝達系の活性調節機構

    • Author(s)
      竹重友貴, 原口省吾, 山本和俊, 筒井和義, 三田雅敏
    • Organizer
      第38回日本比較内分泌学会大会・第40回日本神経内分泌学会学術集会合同大会
    • Place of Presentation
      宮崎市民プラザ (宮崎)
    • Related Report
      2013 Research-status Report
  • [Presentation] Regulatory contribution of Gs-proteins to 1-methyladenine production in starfish ovarian follicle cells stimulated by relaxin-like gonad-stimulating substance

    • Author(s)
      Mita, M., Haraguchi, S., Takeshige, Y., Watanabe, M., and Tsutsui, K.
    • Organizer
      The 7th Intercongress Symposium of Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      National Taiwan Ocean University, Keelung, Taiwan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research2016

    • Author(s)
      Masatoshi Mita (Editors, Yoshio Takei, Hironori Ando and Kazutoshi Tsutsui)
    • Total Pages
      646
    • Publisher
      Elsevier
    • Related Report
      2015 Annual Research Report
  • [Book] Handbook of Hormones2015

    • Author(s)
      Masatoshi Mita
    • Publisher
      Elsevier
    • Related Report
      2014 Research-status Report
  • [Remarks] 国立大学法人 東京学芸大学 自然科学系 教員紹介 三田雅敏

    • URL

      http://univinfo.u-gakugei.ac.jp/u-gakugei/hp/bio-mita1.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京学芸大学 教育学部 自然科学系生命科学分野 三田研究室

    • URL

      http://www.u-gakugei.ac.jp/~bio-mita/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 国立大学法人 東京学芸大学 教員紹介

    • URL

      http://www.u-gakugei.ac.jp/06kyouin/33.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 東京学芸大学教育学部自然科学系生命科学分野 三田研究室

    • URL

      http://www.u-gakugei.ac.jp/~bio-mita/

    • Related Report
      2014 Research-status Report
  • [Remarks] 国立大学法人 東京学芸大学 教員紹介

    • URL

      http://www.u-gakugei.ac.jp/06kyouin/33.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 東京学芸大学教育学部自然科学系生命科学分野 三田研究室

    • URL

      http://www.u-gakugei.ac.jp/~bio-mita/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2022-02-01  

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