Regulatory mechanism of starfish reproduction by relaxin-like gonad-simulating hormone (GSS)
| Project/Area Number |
25440151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Tokyo Gakugei University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生殖腺刺激ホルモン / リラキシン / ペプチドホルモン / オーソログ / シグナル情報伝達機構 / ヒトデ / 生理活性 / 比較内分泌 / 卵成熟誘起ホルモン / 生殖生物学 |
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| Outline of Final Research Achievements |
The potential of ovarian follicle cells to response to GSS was brought by de novo synthesis of Gαs-proteins. On the basis of homology search using conservative nucleotide sequences of relaxin receptor in vertebrates, cDNA for a candidate of GSS receptor was obtained from ovarian follicle cells of the starfish Asterina pectinifera. This study also showed that 1-methyladenine was produced from S-adenosyl-[methyl-13C]-methionine, when follicle cells had been preincubated in [methyl-13C]-methionine. Furthermore, chemical structures of GSS were identified in ten species of starfish. Three kinds of RGP orthologs were found in the class Asteroidea. Based on their cysteine motifs, starfish GSS molecules are classified as member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily and, more precisely, they belong to a relaxin-like peptide family. Therefore, gonadotropin in starfish is designated as relaxin-like gonad-stimulating peptide (RGP).
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] 7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon2015
Author(s)
Haraguchi S, Yamamoto Y, Suzuki Y, Hyung Chang J, Koyama T, Sato M, Mita M, Ueda H, Tsutsui K
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 12546-12546
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Regulatory contribution of Gs-proteins to 1-methyladenine production in starfish ovarian follicle cells stimulated by relaxin-like gonad-stimulating substance
Author(s)
Mita, M., Haraguchi, S., Takeshige, Y., Watanabe, M., and Tsutsui, K.
Organizer
The 7th Intercongress Symposium of Asia and Oceania Society for Comparative Endocrinology
Place of Presentation
National Taiwan Ocean University, Keelung, Taiwan
Related Report
Invited
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