Comparative analyses of hepatic architectures of vertebrates with special attention paid to portal triad development

• Project/Area Number: 25440153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Shizuoka University
• Principal Investigator: Shiojiri Nobuyoshi 静岡大学, 理学部, 教授 (70162568)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 組織構築 / 多様性 / 胆管 / 肝前駆細胞 / ノッチ / 条鰭類 / 進化 / 脊椎動物 / 肝臓構築 / ノッチシグナル / 硬骨魚 / 肝幹細胞

Outline of Final Research Achievements

The hepatic architecture of vertebrates was classified into two types; portal triad-type with intrahepatic bile ducts running along portal veins, and non-portal triad-type with intrahepatic bile ducts independent of portal veins. The latter was observed in many actinopterygians, and the former was seen in other vertebrates, including agnathans, chondrichthyans and mammals. In a process of diversification of actinopterygians, the portal triad-type hepatic architecture gradually changed into the non-portal triad-type hepatic architecture. Fishes of Elopiformes had an intermediate hepatic architecture of both types. Amino acid sequences of Jag1, which is responsible for biliary induction in mammals, were comparatively analyzed among vertebrates with special attention paid to the hepatic architectures.

Research Products

• [Journal Article] Immunohistochemical analyses of cell cycle progression and gene expression of biliary epithelial cells during liver regeneration after partial hepatectomy of the mouse (2016)
  • Author(s): Fukuda, T., Fukuchi, T., Yagi, S. and Shiojiri, N.
  • Journal Title: Exp. Anim. Volume: 65 Pages: 135-146
  • NAID: 130005152400
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Histochemical Analyses of Biliary Development During Metamorphosis of Xenopus laevis Tadpoles. (2015)
  • Author(s): Ueno T, Ishihara A, Yagi S, Koike T, Yamauchi K, Shiojiri N.
  • Journal Title: Zoolog Sci. Volume: 32 Issue: 1 Pages: 88-96
  • DOI: 10.2108/zs140104
  • NAID: 40020363863
  • Peer Reviewed
• [Journal Article] 胆管発生 (2015)
  • Author(s): 塩尻信義
  • Journal Title: 肝胆膵 Volume: 70 Pages: 385-393
  • Acknowledgement Compliant
• [Journal Article] 肝臓構築・機能発現における細胞間相互作用 (2014)
  • Author(s): 塩尻信義
  • Journal Title: 生体の科学 Volume: 65
• [Presentation] 胆管発生と肝特異的転写因子C/EBPα (2015)
  • Author(s): 塩尻信義
  • Organizer: 第42回日本小児栄養消化器肝臓学会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2015-10-17
  • Invited
• [Presentation] 条鰭類における肝臓構築の多様性 (2015)
  • Author(s): 塩尻信義、亀谷治頌、福地智一、安保友香、関口純理、松原幸枝、川上速人
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] 肉鰭類シーラカンスの肝臓の組織構築 (2015)
  • Author(s): 塩尻信義、川上速人
  • Organizer: 第22回肝細胞研究会
  • Place of Presentation: 米子コンベンションセンター（鳥取県米子市）
  • Year and Date: 2015-06-04
• [Presentation] 条鰭類、肉鰭類における肝臓構築の多様性 (2014)
  • Author(s): 塩尻信義
  • Organizer: 第21回日本肝臓医生物学会
  • Place of Presentation: 静岡市・天神の湯大会議室
  • Year and Date: 2014-10-04
• [Presentation] Hepatic cyst formation and abnormal bile duct development in liver-specific Hhex knockout mice (2013)
  • Author(s): Tomoya Ueno, Taichi Yamamoto, Tamio Noguchi, Toru Koike, Nobuyoshi Shiojiri
  • Organizer: The 20th Annual Meeting of the Japanese Society of the Rsearch of Hepatic Cells
  • Place of Presentation: Osaka International Convention Center (大阪市北区)
• [Presentation] Impaired hepatocyte maturation through inactivation of the C/EBPa; gene induces not only abnormal expression of biliary transcription factors but also liver fibrosis
  • Author(s): Nobuyoshi Shiojiri, Toru Koike, Takeshi Oitate, Yusuke Akai
  • Organizer: The 20th Annual Meeting of the Japanese Society of the Rsearch of Hepatic Cells
  • Place of Presentation: Osaka International Convention Center (大阪市北区)
• [Presentation] 下等脊椎動物における肝臓構築
  • Author(s): 塩尻信義
  • Organizer: 第19回日本肝臓医生物学会
  • Place of Presentation: 北海道大学医学部 臨床講義棟第３講堂（札幌市北区）
• [Book] Fetal Development: Stages of Growth, Maternal Influences and Potential Complications (Ed. Josiah WilBurn) (2014)
  • Author(s): Sugiyama, Y., Tomiya, T., Akai, Y., Hattori, S., Koike, T. and Shiojiri, N.
  • Total Pages: 204
  • Publisher: Nova Science Publishers, Inc.
• [Remarks] 塩尻・小池研究室
  • URL: http://liverlovers95.wix.com/shiojiri-koike-lab
• [Remarks] 静岡大学：教員データベース - 塩尻 信義 （SHIOJIRI Nobuyoshi）
  • URL: https://tdb.shizuoka.ac.jp/RDB/public/Default2.aspx?id=11035&l=0