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Studies on functions of membrane steroid receptors and molecular mechanism of oocyte maturation and ovulation

Research Project

Project/Area Number 25440154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionShizuoka University

Principal Investigator

TOKUMOTO Toshinobu  静岡大学, 理学部, 教授 (30273163)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsステロイド膜受容体 / 卵成熟 / 排卵 / 遺伝子発現
Outline of Final Research Achievements

To obtain decisive evidence for the roles of mPRs, we are establishing strains of medaka fish that are deficient in mPRs. In medaka, four subtypes of mPR genes (α, β, γ, and α2) have been identified. By reverse genetic screening, we have selected three to four strains in which a point mutation has been induced in the coding sequence of the mPR subtypes. However, homozygous mutants of each mPR gene showed no phenotype. The results suggested that mPR genes share redundancy. We are currently producing double and triple mutants of the mPR subtypes.
In vivo bioassay which enables to induce oocyte maturation and ovulation in living fish have been applied to select ovulation-inducing genes in this study. Using genome wide microarray of zebrafish, genes that showed ovulation specific elevation were selected. The mRNA expression levels of genes have been confirmed by qPCR analysis. It is indicated that in vivo assay will be a new way to identify ovulation-inducing genes.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (3 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Cell-based assay of nongenomic actions of progestins revealed inhibitory G protein coupling to membrane progestin receptor α (mPRα)2015

    • Author(s)
      Mikiko Nakashima, Manami Suzuki, Misako Saida, Yasuhiro Kamei, Md. Babul Hossain, Toshinobu Tokumoto
    • Journal Title

      Steroids

      Volume: 100 Pages: 21-26

    • DOI

      10.1016/j.steroids.2015.04.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression and purification of human membrane progestin receptor α (mPRα)2015

    • Author(s)
      Md. Babul Hossain, Takayuki Oshima, Shizuka Hirose, Jun Wang, Toshinobu Tokumoto
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 9 Pages: 1-16

    • DOI

      10.1371/journal.pone.0138739

    • NAID

      120006603022

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell-based assay of nongenomic actions of progestins revealed inhibitory G protein coupling to membrane progestin receptor α (mPRα).2015

    • Author(s)
      Mikiko Nakashima, Manami Suzuki, Misako Saida, Yasuhiro Kamei, Md. Babul Hossain, Toshinobu Tokumoto
    • Journal Title

      Steroids

      Volume: 100 Pages: 21-26

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metabolism of bisphenol A by hyper lignin-degrading fungus Phanerochaete sordida YK-624 under non-ligninolytic condition.2014

    • Author(s)
      Jianqiao Wang, Yuto Yamada, Akira Notake, Yasushi Todoroki, Toshinobu Tokumoto, Jing Dong, Peter Thomas, Hirofumi Hirai, Hirokazu Kawagishi
    • Journal Title

      Chemosphere

      Volume: 109 Pages: 128-133

    • DOI

      10.1016/j.chemosphere.2014.01.029

    • NAID

      120005441932

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Purification of the goldfish membrane progestin receptor α (mPRα) expressedin yeast Pichia pastoris2014

    • Author(s)
      Takayuki Oshima, Ryo Nakayama, Shimi Rani Roy, Toshinobu Tokumoto
    • Journal Title

      Biomedical Research

      Volume: 35 Issue: 1 Pages: 47-59

    • DOI

      10.2220/biomedres.35.47

    • NAID

      130004903859

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of pairing in Xenopus by adding steroids into the water, a novel method for pairing.2013

    • Author(s)
      Takehiro Miyazaki, and Toshinobu Tokumoto
    • Journal Title

      Zoological Science

      Volume: 30 Issue: 7 Pages: 565-569

    • DOI

      10.2108/zsj.30.565

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of female-to-male sex reversal in adult zebrafish by fadorozole treatments2013

    • Author(s)
      K Takatsu 他7名
    • Journal Title

      Sci Rep

      Volume: 3 Issue: 1 Pages: 3400-3400

    • DOI

      10.1038/srep03400

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Membrane progestin receptor (mPR) a possible target for new drugs2015

    • Author(s)
      Toshinobu Tokumoto, MD Babul Hossain, Jun Wang, Takehiro Miyazaki
    • Organizer
      9th SFRR-Africa 27-29
    • Place of Presentation
      Hennessy Park Hotel, (Mauritius)
    • Year and Date
      2015-07-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ESTABLISHMENT OF PROCEDURES FOR mPR INTERACTING AGENTS AND STUDY ON PHYSIOLOGICAL ROLES OF mPR2015

    • Author(s)
      Toshinobu Tokumoto
    • Organizer
      9th Rapid Responses to Steroid Hormones
    • Place of Presentation
      Taipei TAIWAN
    • Year and Date
      2015-06-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Establishment of the mutant strains of membrane progestin receptors (mPRs) in the Medaka fish and investigate their central roles2014

    • Author(s)
      himi Rani Roy, Takayuki Oshima, Kaori Miyaoku, Etsuko Sugita, Kanae Takatsu, Miki Ogusu, Babul Hossain, Jun Wang, Ikuyo Hara, Kiyoshi Naruse, Yasuhiro Kamei, Yoshihito Taniguchi, Toshinobu Tokumoto
    • Organizer
      日本比較内分泌学会
    • Place of Presentation
      岡崎コンファレンスセンター, Japan
    • Year and Date
      2014-11-07 – 2014-11-14
    • Related Report
      2014 Research-status Report
  • [Presentation] In vivo induction of oocyte maturation and ovulation in zebrafish; an ideal model to distinguish between genomic and nongenomic actions of steroids2014

    • Author(s)
      Toshinobu Tokumoto
    • Organizer
      Workshop in Ramkhamhaeng University
    • Place of Presentation
      Ramkhamhaeng University, Thailand
    • Year and Date
      2014-10-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Towards elucidation of the roles of membrane progestin receptors (mPRs) in the non-genomic steroidal actions.2013

    • Author(s)
      Toshinobu Tokumoto
    • Organizer
      Rapid Responses to Steroid Hormones 8th International Meeting
    • Place of Presentation
      Eri, PA, USA
    • Related Report
      2013 Research-status Report
  • [Book] ナノバイオ・テクノロジー 「ステロイドホルモンの新しい作用のしくみ」2016

    • Author(s)
      徳元俊伸 他
    • Total Pages
      267
    • Publisher
      静岡学術出版
    • Related Report
      2015 Annual Research Report
  • [Book] Zebrafish: Topics in Reproduction and Development2014

    • Author(s)
      Toshinobu Tokumoto (C.A Lessman and E. Carver ed)
    • Total Pages
      300
    • Publisher
      NOVA Science Publishers Inc.
    • Related Report
      2013 Research-status Report
  • [Book] Sexual Plasticity and Gametogenesis in Fishes2013

    • Author(s)
      Toshinobu Tokumoto (B.Senthilkumaran ed)
    • Total Pages
      468
    • Publisher
      NOVA Science Publishers Inc.
    • Related Report
      2013 Research-status Report
  • [Remarks] ステロイド膜受容体の機能解明と創薬に向けた研究

    • URL

      https://www.youtube.com/watch?v=9Bf6a7fO1qA

    • Related Report
      2014 Research-status Report
  • [Remarks] プロゲスチン膜受容体の人工合成系と分離精製法の確立

    • URL

      http://gsst.shizuoka.ac.jp/kyoiku/hakase.html?bn=2013_Oshima#player

    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] ステロイドホルモン膜受容体の精製方法2013

    • Inventor(s)
      徳元俊伸、大島卓之
    • Industrial Property Rights Holder
      静岡大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-136980
    • Filing Date
      2013-06-28
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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