Studies on functions of membrane steroid receptors and molecular mechanism of oocyte maturation and ovulation
Project/Area Number |
25440154
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | Shizuoka University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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Keywords | ステロイド膜受容体 / 卵成熟 / 排卵 / 遺伝子発現 |
Outline of Final Research Achievements |
To obtain decisive evidence for the roles of mPRs, we are establishing strains of medaka fish that are deficient in mPRs. In medaka, four subtypes of mPR genes (α, β, γ, and α2) have been identified. By reverse genetic screening, we have selected three to four strains in which a point mutation has been induced in the coding sequence of the mPR subtypes. However, homozygous mutants of each mPR gene showed no phenotype. The results suggested that mPR genes share redundancy. We are currently producing double and triple mutants of the mPR subtypes. In vivo bioassay which enables to induce oocyte maturation and ovulation in living fish have been applied to select ovulation-inducing genes in this study. Using genome wide microarray of zebrafish, genes that showed ovulation specific elevation were selected. The mRNA expression levels of genes have been confirmed by qPCR analysis. It is indicated that in vivo assay will be a new way to identify ovulation-inducing genes.
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Report
(4 results)
Research Products
(18 results)
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[Presentation] Establishment of the mutant strains of membrane progestin receptors (mPRs) in the Medaka fish and investigate their central roles2014
Author(s)
himi Rani Roy, Takayuki Oshima, Kaori Miyaoku, Etsuko Sugita, Kanae Takatsu, Miki Ogusu, Babul Hossain, Jun Wang, Ikuyo Hara, Kiyoshi Naruse, Yasuhiro Kamei, Yoshihito Taniguchi, Toshinobu Tokumoto
Organizer
日本比較内分泌学会
Place of Presentation
岡崎コンファレンスセンター, Japan
Year and Date
2014-11-07 – 2014-11-14
Related Report
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