Regulation of folliculegenesis by brain/gut peptides in Ciona intestinalis

• Project/Area Number: 25440161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Suntory Foundation for Life Sciences
• Principal Investigator: Satake Honoo 公益財団法人サントリー生命科学財団, その他部局等, 主幹研究員 (20280688)
• Co-Investigator(Renkei-kenkyūsha): KAWADA Tsuyoshi 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (90300821)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ペプチド / カタユウレイボヤ / 卵胞成長 / 進化 / ホヤ / 脳腸ペプチド / 卵胞 / Wnt / in situ hybridization / RNA-Seq

Outline of Final Research Achievements

In this study, we identified a receptor for Ci-GALP, which is an Ciona intestin ortholog of vertebrate galanin/GALP. We also localized the mRNA ofreceptors for Ci-GALP, cionin, and GrRH, respectivly. RNA-Seq follwed by real-time PCR for the Ciona follicles treated with each peptide revealed the gene expression profile. Moreover, the inhibitors of the regulated gene product suppress follicle grwoth in a ligand-specific manner. Taken together, these results substantiated the essentila molecular mechanisms of Ciona follicle grwoth regulated bu brain/gut peptides. These are the first reports on the biological roles of these brain/gut peptide in the ovary in an organism.

Research Products

• [Journal Article] Evidence for conservation of the calcitonin superfamily and activity-regulating mechanisms in the basal chordate Branchiostoma floridae: insight into the molecular and functional evolution in chordates. (2016)
  • Author(s): Sekiguchi, T., Kuwasako, K., Ogasawara, M., Takahashi, H., Matsubara, S., Osugi, T., Muramatsu, I., Sasayama, Y., Suzuki, N., and Satake, H.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 5 Pages: 2345-2356
  • DOI: 10.1074/jbc.m115.664003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Matsubara S, Kawada T, Sakai T, Aoyama M, Osugi T, Shiraishi A, Satake H. (2016)
  • Author(s): The significance of Ciona intestinalis as a stem organism in integrative studies of functional evolution of the chordate endocrine, neuroendocrine, and nervous systems.
  • Journal Title: Gen Comp Endocrinol. Volume: 227 Pages: 101-108
  • DOI: 10.1016/j.ygcen.2015.05.010
  • Peer Reviewed
• [Journal Article] Generation of Triple-Transgenic Forsythia Cell Cultures as a Platform for the Efficient, Stable, and Sustainable Production of Lignans. (2015)
  • Author(s): 2.Murata J, Matsumoto E, Morimoto K, Koyama T, Satake H.
  • Journal Title: PLOS One Volume: 10 Issue: 12 Pages: e0144519-e0144519
  • DOI: 10.1371/journal.pone.0144519
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of GnRH-like peptides from the nerve ganglia of Yesso scallop, Patinopecten yessoensis. (2015)
  • Author(s): Nagasawa, K, Osugi, T, Suzuki I, Itoh N, Takahashi KG, Satake H, Osada M
  • Journal Title: Peptides Volume: 71 Pages: 202-210
  • DOI: 10.1016/j.peptides.2015.07.022
  • Peer Reviewed
• [Journal Article] The Effect of Chitosan and Chitin Oligomers on Gene Expression and Lignan Production in Linum album Cell Cultures. (2014)
  • Author(s): Bahabadi, S.E., Sharifi, M., Murata, J. Satake, H.
  • Journal Title: J. Med. Plant. Volume: １３ Pages: 46-53
  • Peer Reviewed / Open Access
• [Journal Article] A conserved noncoding sequence can function as a spermatocyte-specific enhancer and a bidirectional promoter for a ubiquitously expressed gene and a testis-specific long noncoding RNA. (2014)
  • Author(s): Kurihara, M., Shiraishi, A., Satake, H., Kimura A.P.
  • Journal Title: J. Mol. Biol. Volume: ４２６ Issue: 17 Pages: 3069-3093
  • DOI: 10.1016/j.jmb.2014.06.018
  • NAID: 120005476218
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis. (2014)
  • Author(s): Kamiya C, Ohta N, Ogura Y, Yoshida K, Horie T, Kusakabe TG, Satake H, Sasakura Y.
  • Journal Title: Ｄｅｖｅｌｏｐｍｅｎｔａｌ Ｄｙｎａｍｉｃｓ Volume: 243 Issue: 12 Pages: 1524-1535
  • DOI: 10.1002/dvdy.24176
  • Peer Reviewed / Open Access
• [Journal Article] Significant enhancement of lignans accumulation in hairy root cultures of Linum album using biotic elicitors (2014)
  • Author(s): Bahabadi, S.E., Sharifi, M., Chashmi, N.A. Murata, J. Satake, H.
  • Journal Title: Acta Physiol. Plant. Volume: ３６ Issue: 12 Pages: 3325-3331
  • DOI: 10.1007/s11738-014-1700-z
  • Peer Reviewed / Open Access
• [Journal Article] The Draft Genome of Hop (Humulus lupulus), an Essence for Brewing. (2014)
  • Author(s): Natsume, S., Takagi, H., Shiraishi, A., Murata, J., Toyonaga, H., Patzak, J., Takagi, M., Yaegashi, H., Uemura, A., Mitsuoka, C., Yoshida, K., Krofta, K., Satake, H., Terauchi, R., Ono, E.
  • Journal Title: Plant Cell Physiol. Volume: ５６ Issue: 3 Pages: 428-441
  • DOI: 10.1093/pcp/pcu169
  • Peer Reviewed / Open Access
• [Journal Article] Seasonal Alteration in Amounts of Lignans and Their Glucosides and Gene Expression of the Relevant Biosynthetic Enzymes in the <i>Forsythia suspense</i> Leaf (2013)
  • Author(s): Morimoto, K., Satake, H.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 36 Issue: 9 Pages: 1519-1523
  • DOI: 10.1248/bpb.b13-00437
  • NAID: 130003361535
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element. (2013)
  • Author(s): Akiko Hozumi
  • Journal Title: Developmental Dynamics Volume: 242 Issue: 1 Pages: 30-43
  • DOI: 10.1002/dvdy.23891
  • Peer Reviewed
• [Journal Article] The pars intercerebralis affects digestive activities of the American cockroach, Periplaneta americana, via crustacean cardioactive peptide and allatostatin-A. (2013)
  • Author(s): Matsui, T., Sakai, T., Satake, H., Takeda M.
  • Journal Title: J. Insect Physiol. Volume: 59 Issue: 1 Pages: 33-37
  • DOI: 10.1016/j.jinsphys.2012.06.010
  • Peer Reviewed
• [Journal Article] GPCR Heterodimerization in the Reproductive System: Functional Regulation and Implication for Biodiversity. (2013)
  • Author(s): Satake H, Matsubara S, Aoyama M, Kawada T, Sakai T.
  • Journal Title: Front Endocrinol Volume: 4 Pages: 100-100
  • DOI: 10.3389/fendo.2013.00100
  • Peer Reviewed
• [Journal Article] Recent advances in metabolic engineering of lignan biosynthesis pathways for the production of transgenic plant-based foods and supplements (2013)
  • Author(s): Satake, H., Ono, E., Murata, J.
  • Journal Title: J. Agric. Food Chem. Volume: 61 Issue: 48 Pages: 11721-11729
  • DOI: 10.1021/jf4007104
  • Peer Reviewed
• [Journal Article] Insight into Molecular and Functional Diversity of Tachykinins and their Receptors. (2013)
  • Author(s): Satake, H., Aoyama, M., Sekiguchi, T., Kawada, T.
  • Journal Title: Protein & Peptide Letters Volume: 20 Issue: 6 Pages: 615-627
  • DOI: 10.2174/0929866511320060002
  • Peer Reviewed
• [Presentation] カタユウレイボヤを起点とした脊索動物門に共通する卵胞制御機構の新展開 (2015)
  • Author(s): 佐竹炎
  • Organizer: 第86回日本動物学会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] GnRH promotes ovarian follicular growth in Ciona intestinalis (2014)
  • Author(s): 松原、白石、佐竹
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] The distribution of multiple neuropeptides were visualized in the brain of Ciona intestinalis by imaging mass spectrometry. (2014)
  • Author(s): 大杉、川田、佐竹
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] 脊索動物門で保存されている新規卵胞制御機構の共通性と多様性の解明 (2014)
  • Author(s): 佐竹
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] 成長途上のカタユウレイボヤ卵巣におけるホヤバソプレシン遺伝子の発現解析 (2014)
  • Author(s): 川田、堀江、笹倉、小笠原、佐竹
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ホヤのコレシストキニン/ガストリンホモログcioninの卵巣における機能解析 (2014)
  • Author(s): 大杉、白石、佐竹
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] GPCRヘテロダイマーの半網羅的検出方法の確立 (2014)
  • Author(s): 松原、酒井、佐竹
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 脊索動物門における生殖内分泌系の共通性と多様性に迫る-ホヤのペプチドホルモン研究を出発として- (2013)
  • Author(s): 佐竹、川田、宮坂、大杉、松原、白石、青山、安田
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Differential regulation of GnRH signalling cascades via heterodimerization among multiple GnRH receptor palarogs in the ascidian, Ciona intestinalis. (2013)
  • Author(s): Satake H
  • Organizer: 17th International Congress of Comparative Endocrinology
  • Place of Presentation: Barcelona
  • Invited
• [Presentation] マウスにおいてタキキニンは二次卵胞成長を促進する (2013)
  • Author(s): 青山、川田、伊丹、保、安田、佐竹
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎
• [Presentation] トランスジェニック体を用いたカタユウレイボヤのオキシトシン/バソプレシン遺伝子の発現解析 (2013)
  • Author(s): 川田、堀江、笹倉、小笠原、佐竹
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
• [Presentation] GPCRに普遍的な相互作用パターン抽出による生物種ワイドな相互作用予測法の開発 (2013)
  • Author(s): 白石、佐竹
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Remarks]