| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This research project aims to make a prototype of a bio-inspired electric nose, which can detect different types of odorant molecules and distinguish them. In order to use the sensing-element of the electric nose, we plan to use odorant binding proteins of Japanese common newt, Cp-Lip1 and -Lip2 which we found and chemosensory proteins (CSPs) found in the sensillum of carpenter ant, Camponotus japonicus. The expression and purification system of the proteins from E. coli expression systems were established.The purified proteins retained their native secondary structure and binding properties. The amino acid replacement of Cp-Lip1 changed its odorant binding properties, which enable to obtain odorant-sensor elements of the different odorant-binding capacity. These sensor elements were fixed and used on a prototype of a surface acoustic wave (SAW) sensor and a field effect transistor (FET) sensor.
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