Behavioural physiological analyses of ohototaxis of the marbled crayfish

• Project/Area Number: 25440165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Yamagata University
• Principal Investigator: NAGAYAMA Toshiki 山形大学, 理学部, 教授 (80218031)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生得的行動 / 習得的行動 / 走性 / 定位 / 光 / 学習 / 可塑性 / マーブルクレイフィッシュ / 神経スイッチ / 行動 / 二次メッセンジャー系 / ｃAMP / セロトニン / ドーパミン / 薬理行動学

Outline of Final Research Achievements

Phototaxis is a one of essential elements for innate behaviour. Phototactic behaviours are observed from prokaryotes to amphibian and basic form of orientation. marbled crayfish are shown phototaxis in which sign reversed from negative to positive depending on external light conditionings. LD animals reared with 12L/12D light cycle showed negative phototaxis during day-time and positive phototaxis during night-time. DD animals reared under constant dark conditioning showed positive phototaxis during day-time. Injection of serotonin caused a reversed positive phototaxis in LD animals while dopamine caused a reversed negative phototaxis in DD animals. Injection of serotonin 5HT1 receptor antagonist blocked the reverse of phototaxis from negative to positive, while dopamine DA1 receptor antagonist blocked the reverse from positive to negative. Serotonin mediates positive phototaxis owing decrease in cAMP level while dopamine mediated negative phototaxis owing increase in cAMP level.

Research Products

• [Journal Article] Habituation of backward escape swimming in the marbled crayfish (2016)
  • Author(s): Kasuya Azusa and Nagayama Toshiki
  • Journal Title: Zoological Science Volume: 33 Issue: 1 Pages: 6-12
  • DOI: 10.2108/zs150099
  • NAID: 40020738709
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two types of orienting behaviour during agaonistic encounters in the crayfish Procambarus clarkii (Decapoda:Cambaridae) (2016)
  • Author(s): Watanabe Susumu, Momohara Yuto, Minami Hiroki and Nagayama Toshiki
  • Journal Title: Journal of Crustacean Biology Volume: 36 Issue: 2 Pages: 147-153
  • DOI: 10.1163/1937240x-00002411
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Habituation of LG-mediated tailflip in the crayfish (2015)
  • Author(s): Nagayama Toshiki and Araki Makoto
  • Journal Title: Invertebrate Neuroscience Volume: 15:2 Issue: 2 Pages: 1-12
  • DOI: 10.1007/s10158-015-0178-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Excitatory connections of nonspiking interneurones in the terminal abdominal ganglion of the crayfish (2015)
  • Author(s): Namba Hisaaki and Nagayama Toshiki
  • Journal Title: Journal of Comparative Physiology A Volume: 201 Issue: 8 Pages: 773-781
  • DOI: 10.1007/s00359-015-1017-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Serotonergic modulation of social status-dependent behavioural plasticity of the crayfish avoidance reaction (2015)
  • Author(s): Momohara Yuto, Yoshida Misaki and Nagayama Toshiki
  • Journal Title: Journal of Comparative Physiology A Volume: 201 Issue: 11 Pages: 1063-1074
  • DOI: 10.1007/s00359-015-1038-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nitric oxide modulates a swimmeret beating rhythm in the crayfish (2014)
  • Author(s): Atsuki Mita, Misaki Yoshida and Toshiki Nagayama
  • Journal Title: Journal of Experimental Biology Volume: 217 Pages: 4423-4431
  • DOI: 10.1242/jeb.110551
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Social status-dependent modulation of LG-flip habituation in the crayfish. (2013)
  • Author(s): Araki M., Hasegawa T., Komatsuda S. and Nagayama T.
  • Journal Title: J. Exp Biol. Volume: 216 Pages: 681-686
  • Peer Reviewed
• [Journal Article] Aminergic control of social status in crayfish agonistic encounters. (2013)
  • Author(s): Momohara Y., Kanai A. and Nagayama T.
  • Journal Title: PLoS One Volume: 8(9) e74489 Pages: 1-12
  • Peer Reviewed
• [Presentation] 闘争時のザリガニの優劣形成 (2015)
  • Author(s): 長山俊樹
  • Organizer: 日本比較生理生化学会第37回大会
  • Place of Presentation: 広島
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] A prior resident effect of the marbled crayfish (2015)
  • Author(s): Kazuya Takahashi and Toshiki Nagayama
  • Organizer: 日本比較生理生化学会第37回大会
  • Place of Presentation: 広島
  • Year and Date: 2015-12-11
• [Presentation] ザリガニ個体間地位形成に伴うノンスパイキング介在ニューロンの応答変化 (2015)
  • Author(s): 小山大河、長山俊樹
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-17
• [Presentation] ザリガニ遊泳肢律動における神経節間同期性 (2015)
  • Author(s): 吉田美咲、長山俊樹
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-17