Overviewing their dynamics of peptidergic neuromodulation: From single neuron to neuronal circuit

• Project/Area Number: 25440166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Nagoya University
• Principal Investigator: Abe Hideki 名古屋大学, 生命農学研究科, 准教授 (90396804)
• Co-Investigator(Renkei-kenkyūsha): OKA YOSHITAKA 東京大学, 理学系研究科, 教授 (70143360)
• Research Collaborator: SHIGA MASAKATSU ; GOTO YURI ; UMATANI CHIE
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ペプチド放出 / 神経修飾 / GnRH / 細胞培養 / live cell imaging / 嗅球 / 視蓋 / ペプチド / 開口放出 / イメージング / 初代培養 / 神経ペプチド / ライブセルイメージング / 共培養 / 視覚神経回路

Outline of Final Research Achievements

To understand peptide release from single peptidergic neuron and their neuromodulatory actions on their target neuronal circuit, we performed following studies using fish terminal nerve-GnRH3 peptidergic neurons as a model: (i) establishment of the co-culture system of terminal nerve GnRH3 neurons and olfactory bulbar neurons that receive neuromodulatory actions of GnRH3 peptide by dissociating goldfish olfactory bulb. (ii) live-cell imaging of secretory vesicles transfer and their exocytosis from single cultured TN-GnRH3 neuron by exogenous expression of the fluorescent exocytotic sensor proteins, (iii) recording of visual stimuli (static/moving small objects)-induced single neuronal activity in in vivo from the optic tectum of immobilized medaka, and (iv) whole-cell patch-clamp recording of the enhancement of Ca2+-induced K+ current by GnRH3 peptide that will inhibit subventricular neuronal activities of the optic tectum.

Research Products

• [Journal Article] Morphological analysis of the early development of telencephalic and diencephalic gonadotropin-releasing hormone neuronal systems in the enhanced green fluorescent protein-expressing transgenic medaka lines. (2016)
  • Author(s): Takahashi A, Islam MS, Abe H, Okubo K, Akazome Y, Kaneko T, Hioki H, *Oka Y.
  • Journal Title: J Comp Neurol Volume: 524 Issue: 4 Pages: 896-913
  • DOI: 10.1002/cne.23883
  • Peer Reviewed / Open Access
• [Journal Article] GnRH suppresses excitability of visual processing neurons in the optic tectum. (2015)
  • Author(s): Chie Umatani, Ryosuke Misu, Shinya Oishi, Kazuhiko Yamaguchi, Hideki Abe, Yoshitaka Oka
  • Journal Title: Journal of Neurophysiology Volume: 114 Issue: 5 Pages: 2775-2784
  • DOI: 10.1152/jn.00710.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Connections of the commissural nucleus of Cajal in the goldfish with special reference to the topographic organization of ascending visceral sensory pathways (2015)
  • Author(s): Uezono S, Yamada Y, Kato T, Abe H, Yamamoto N.
  • Journal Title: J Comp. Neurol. Volume: 523 Issue: 2 Pages: 209-225
  • DOI: 10.1002/cne.23675
  • Peer Reviewed
• [Journal Article] A neural mechanism underlying mating preferences for familiar individuals in medaka fish. (2014)
  • Author(s): Okuyama T, Yokoi S, Abe H, Isoe Y, Suehiro Y, Imada H, Tanaka M, Kawasaki T, Yuba S, Taniguchi Y, Kamei Y, Okubo K, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H
  • Journal Title: Science (New York, N.Y.) Volume: 343 Issue: 6166 Pages: 91-94
  • DOI: 10.1126/science.1244724
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intrinsic photosensitivity of deep brain photoreceptor (2014)
  • Author(s): Nakane, Y., Shimmura, T., Abe, H. and Yoshimura, T.
  • Journal Title: Current Biology Volume: 24 Issue: 13 Pages: R596-R597
  • DOI: 10.1016/j.cub.2014.05.038
  • Peer Reviewed
• [Journal Article] Kiss1 neurons drastically change their firing activity in accordance with the reproductive state: insights from a seasonal breeder. (2014)
  • Author(s): Hasebe M, Kanda S, Shimada H, Akazome Y, Abe H, Oka Y.
  • Journal Title: Endocrinology Volume: 155 Issue: 12 Pages: 4868-4880
  • DOI: 10.1210/en.2014-1472
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Whole brain-pituitary in vitro preparation of the transgenic medaka (Oryzias latipes) as a tool for analyzing the differential regulatory mechanisms of LH and FSH release. (2014)
  • Author(s): Karigo T, Aikawa M, Kondo C, Abe H, Kanda S, Oka Y.
  • Journal Title: Endocrinology Volume: 155 Issue: 2 Pages: 536-547
  • DOI: 10.1210/en.2013-1642
  • Peer Reviewed
• [Journal Article] The saccus vasculosus of fish is a sensor of seasonal changes in day length (2013)
  • Author(s): Nakane, Y., Ikegami, K., Iigo, M., Ono, H., Takeda, K., Takahashi, D., Uesaka, M., Kimijima, M., Hashimoto, R., Arai, N., Suga, T., Kosuge, K., Abe, T., Maeda, R., Senga, T., Amiya, N., Azuma, T., Amano, M., Abe, H., Yamamoto, N., Yoshimura, T.
  • Journal Title: Nature Communications Volume: 4, Article 2108 Issue: 1 Pages: 1-7
  • DOI: 10.1038/ncomms3108
  • Peer Reviewed
• [Journal Article] Burst generation mediated by cholinergic input in terminal nerve-gonadotrophin releasing hormone neurones of the goldfish (2013)
  • Author(s): Kawai, T., Abe, H., and Oka, Y.
  • Journal Title: Journal of Physiology-London Volume: 591 Issue: 22 Pages: 5509-5523
  • DOI: 10.1113/jphysiol.2013.258343
  • Peer Reviewed
• [Presentation] メダカ視蓋ニューロン視覚応答特性のin vivo記録 (2015)
  • Author(s): 後藤友里・阿部秀樹
  • Organizer: 第11回水生動物の行動と神経系シンポジウム
  • Place of Presentation: 横浜市立大学医学部
  • Year and Date: 2015-12-05
• [Presentation] 硬骨魚類における中枢視覚路の進化 (2015)
  • Author(s): 萩尾華子、佐藤萌、阿部秀樹、山本直之
  • Organizer: 第11回水生動物の行動と神経系シンポジウム
  • Place of Presentation: 横浜市立大学医学部
  • Year and Date: 2015-12-05
• [Presentation] Evolution of the ascending visual pathways in teleosts (2015)
  • Author(s): Hagio, H. Satou, M. Abe, H. and Yamamoto, N.
  • Organizer: 45th Annual Meetings, Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Neuromodulation of retino-tectal neurotransmission via extrahypothalamic GnRH neurons (2015)
  • Author(s): Umatani, C. Misu, R. Oishi, S. Abe, H. and Oka, Y.
  • Organizer: 45th Annual Meetings, Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] 脱分極刺激によって生じたキンギョ培養終神経GnRH3ニューロンにおける分泌小胞移動変化 (2015)
  • Author(s): 志賀将雄・阿部秀樹
  • Organizer: 日本動物学会8６回大会
  • Place of Presentation: 朱雀メッセ新潟コンベンションセンター
  • Year and Date: 2015-09-17
• [Presentation] Neuromodulation in fish visual system via terminal nerve GnRH neurons (2015)
  • Author(s): Umatani, C., Misu, R., Oishi, S., Abe, H., Oka, Y.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-07-28
• [Presentation] In vitro culture system of goldfish terminal nerve GnRH3 peptidergic neurons for studying peptide release and their neuromodulation mechanisms (2015)
  • Author(s): Abe, H. and Shiga, M.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-07-28
• [Presentation] 繁殖行動を制御する脳内ペプチドニューロンによる神経修飾機構を探るためのキンギョ嗅球・終神経GnRHニューロンの共培養系 (2014)
  • Author(s): 志賀将雄・阿部秀樹・山本直之
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] キンギョ培養終神経GnRH ニューロンに遺伝子導入した開口放出センサータンパク質 (2014)
  • Author(s): 志賀将雄・阿部秀樹
  • Organizer: 日本動物学会８５回大会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Neuromodulatory effects of terminal nerve GnRH neurons in the fish visual system (2014)
  • Author(s): Umatani C, Abe H, Oka Y
  • Organizer: International Congress for Neuroethology
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] A neural mechanism underlying mating preferences for familiar individuals in medaka fish (2014)
  • Author(s): Teruhiro Okuyama, Saori Yokoi, Hideki Abe, Yasuko Isoe, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Shunsuke Yuba, Yoshihito Taniguchi, Yasuhiro Kamei, Kataaki Okubo, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi
  • Organizer: 2nd Medaka Strategic Meeting
  • Place of Presentation: Seville, Spain
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] メダカの配偶者選択の分子神経基盤 (2013)
  • Author(s): 奥山輝大，横井佐織，阿部秀樹，磯江泰子，末廣勇司，今田はるか，島田敦子，川崎隆史，弓場俊輔，谷口善仁，亀井保博，田中実，成瀬清，武田洋幸，岡良隆，久保健雄，竹内秀明
  • Organizer: 日本動物学会第84会大会
  • Place of Presentation: 岡山大学
• [Presentation] 終神経GnRH ニューロンが視覚神経回路に及ぼす作用の解析 (2013)
  • Author(s): 馬谷千恵・阿部秀樹・岡良隆
  • Organizer: 日本動物学会第84会大会
  • Place of Presentation: 岡山大学
• [Presentation] 開口放出・神経修飾機構解析を目的としたキンギョ終神経GnRHニューロン初代培養系 (2013)
  • Author(s): 阿部秀樹・志賀将雄
  • Organizer: 日本動物学会第84会大会
  • Place of Presentation: 岡山大学
• [Presentation] Morphological analysis of the early development of GnRH neuron systems in the EGFP-expressing transgenic medaka lines (2013)
  • Author(s): Takahashi, A., Sadiqul, I., Akazome, Y., Abe, H., Okubo, K., and Oka, Y.
  • Organizer: 43rd Annual Meetings, Society for Neuroscience
  • Place of Presentation: San Diego
• [Presentation] Ascending general visceral sensory pathways from the commissural nucleus of Cajal in goldfish (2013)
  • Author(s): Uezono, S., Yamada, Y., Abe, H. and Yamamoto, N.
  • Organizer: 43rd Annual Meetings, Society for Neuroscience
  • Place of Presentation: San Diego
• [Presentation] 神経修飾ペプチドの放出および放出されたペプチドによる神経修飾機構解析のためのキンギョ終神経GnRHニューロン・嗅球構成細胞共培養系 (2013)
  • Author(s): 志賀将雄・阿部秀樹
  • Organizer: 第10回水棲動物の行動と神経系シンポジウム
  • Place of Presentation: 鹿児島大学
• [Presentation] Neural mechanism underlying socially-regulated female mating receptivity in medaka fish (2013)
  • Author(s): Teruhiro Okuyama, Saori Yokoi, Hideki Abe, Yasuko Isoe, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Shunsuke Yuba, Yoshihito Taniguchi, Yasuhiro Kamei, Kataaki Okubo, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi
  • Organizer: 第36 回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Remarks] 名古屋大学大学院生命農学研究科水圏動物学研究分野
  • URL: https://www.agr.nagoya-u.ac.jp/~hikaku/
• [Remarks] 水圏動物学研究分野神経生理学研究グループ（阿部）
  • URL: https://lfbphysiol.wordpress.com/
• [Remarks] 名古屋大学大学院生命農学研究科水圏動物学研究分野
  • URL: http://www.agr.nagoya-u.ac.jp/~hikaku/
• [Remarks] 水圏動物学研究分野神経生理学研究グループ（阿部）
  • URL: https://lfbphysiol.wordpress.com
• [Remarks] 名古屋大学大学院生命農学研究科水圏動物学研究分野
  • URL: http://www.agr.nagoya-u.ac.jp/~hikaku/
• [Remarks] 水圏動物学研究分野神経生理学研究グループ（阿部）
  • URL: http://lfbphysiol.wordpress.com/