Analysis of the photoreceptive protein Opn5 found in invertebrates

• Project/Area Number: 25440167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Kyoto University
• Principal Investigator: YAMASHITA TAKAHIRO 京都大学, 理学(系)研究科(研究院), 助教 (50378535)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光受容 / オプシン / シグナル伝達

Outline of Final Research Achievements

Opn5 constitutes one of the photoreceptive protein, opsin, groups, whose gene is widely distributed in deuterostome genomes. We previously revealed that vertebrate Opn5 is classified into several subgroups and is diversified based on their molecular properties and tissue distribution patterns. In this study, we analyzed Opn5 found from deuterostomes other than vertebrates. We successfully obtained the recombinant proteins of these Opn5 proteins after reconstituted with 11-cis-retinal. The molecular properties of these Opn5 proteins shared with those of several vertebrate Opn5 subgroups. In addition, we detected Opn5 expression signals both during early development and in adult of sea urchin. These data suggest that sea urchin utilizes Opn5 in a variety of developmental stages to respond to light environment.

Research Products

• [Journal Article] Diversity of active states in TMT opsins (2015)
  • Author(s): K. Sakai
  • Journal Title: PLoS One Volume: 10 Issue: 10 Pages: 1-12
  • DOI: 10.1371/journal.pone.0141238
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Origin of the low thermal isomerization rate of rhodopsin chromophore (2015)
  • Author(s): Yanagawa, M.; Kojima, K.; Yamashita, T.; Iwamoto, Y.; Matsuyama, T.; Nakanishi, K.; Yamano, Y.; Wada, A.; Sako, Y.; Shichida, Y.
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 11081-11081
  • DOI: 10.1038/srep11081
  • NAID: 120005690204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 代謝型グルタミン酸受容体の分子構造と機能 (2015)
  • Author(s): 山下高廣
  • Journal Title: Clinical Neuroscience（中外医学社） Volume: 33 Pages: 25-7
• [Journal Article] Contribution of glutamic acid in the conserved E/DRY triad to the functional properties of rhodopsin. (2014)
  • Author(s): Sato, K., Yamashita, T., and Shichida, Y.
  • Journal Title: Biochemistry Volume: 53 Issue: 27 Pages: 4420-4425
  • DOI: 10.1021/bi5003772
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evolution of mammalian opn5 as a specialized UV-absorbing pigment by a single amino acid mutation (2014)
  • Author(s): Yamashita, T., Ono, K., Ohuchi, H., Yumoto, A., Gotoh, H., Tomonari, S., Sakai, K., Fujita, H., Imamoto, Y., Noji, S., Nakamura, K., and Shichida, Y.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 7 Pages: 3991-4000
  • DOI: 10.1074/jbc.m113.514075
  • NAID: 120005690203
  • Peer Reviewed
• [Journal Article] Rod visual pigment optimizes active state to achieve efficient G protein activation as compared to cone visual pigments (2014)
  • Author(s): Kojima, K., Imamoto, Y., Maeda, R., Yamashita, T., and Shichida, Y
  • Journal Title: J. Biol. Chem Volume: 289 (8) Issue: 8 Pages: 5061-5073
  • DOI: 10.1074/jbc.m113.508507
  • Peer Reviewed
• [Journal Article] Ligand-induced population shift leads to the activation of rhodopsin, a G-protein coupled receptor (2014)
  • Author(s): Maeda, R., Hiroshima, M., Yamashita, T., Wada, A., Nishimura, S., Sako, Y., Shichida, Y., and Imamoto, Y
  • Journal Title: Biophys. J Volume: 106 (4) Issue: 4 Pages: 915-924
  • DOI: 10.1016/j.bpj.2014.01.020
  • Peer Reviewed
• [Journal Article] "Chimeric proton-pumping rhodopsin containing the cytoplasmic loop of bovine rhodopsin" (2014)
  • Author(s): K. Sasaki, T. Yamashita, K. Yoshida, K. Inoue, Y. Shichida, H. Kandori
  • Journal Title: PLOS ONE Volume: 9 Issue: 3 Pages: 91323-91323
  • DOI: 10.1371/journal.pone.0091323
  • Peer Reviewed
• [Journal Article] Efficiencies of activation of transducin by cone and rod visual pigments (2013)
  • Author(s): Imamoto, Y., Seki, I., Yamashita, T., and Shichida, Y
  • Journal Title: Biochemistry Volume: 52 (17) Issue: 17 Pages: 3010-3018
  • DOI: 10.1021/bi3015967
  • Peer Reviewed
• [Presentation] Molecular Properties of a Universal UV-light Sensitive Opsin in Vertebrates (2016)
  • Author(s): T. Yamashita
  • Organizer: Gordon Research Conferences
  • Place of Presentation: ガルベストン（アメリカ）
  • Year and Date: 2016-01-25
  • Int'l Joint Research / Invited
• [Presentation] 哺乳類光受容タンパク質Opn5の分子特性と発現部位の解析 (2015)
  • Author(s): 山下高廣
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2015-09-17
• [Presentation] Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation (2015)
  • Author(s): T. Yamashita
  • Organizer: FASEB meeting
  • Place of Presentation: ビッグスカイ（アメリカ）
  • Year and Date: 2015-06-17
  • Int'l Joint Research
• [Presentation] Molecular properties of brain photoreceptor protein Opn5 (2015)
  • Author(s): Takahiro Yamashita
  • Organizer: RNA & Clock 2015
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2015-03-25
  • Invited
• [Presentation] Molecular properties of vertebrate non-visual opsin, Opn5 (2014)
  • Author(s): Takahiro Yamashita
  • Organizer: 16th International Conference on Retinal Proteins
  • Place of Presentation: 長浜ロイヤルホテル
  • Year and Date: 2014-10-08
  • Invited
• [Presentation] Diversity of molecular properties of vertebrate non-visual opsin Opn5 (2014)
  • Author(s): Takahiro Yamashita
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] 脊椎動物新規紫外光受容タンパク質Opn5の解析 (2014)
  • Author(s): 山下高廣
  • Organizer: 第18回光生物学協会年会
  • Place of Presentation: 大阪市立大
  • Year and Date: 2014-08-23
  • Invited
• [Presentation] 視覚以外の機能に関わるロドプシン類の分子特性 (2014)
  • Author(s): 山下高廣
  • Organizer: 第11回GPCR研究会
  • Place of Presentation: 日本科学未来館
  • Year and Date: 2014-05-10
  • Invited
• [Presentation] Diversity of molecular properties of vertebrate non-visual opsin group, Opn5
  • Author(s): T. Yamashita
  • Organizer: The 6th Asia and Oceania Conference on Photobiology
  • Place of Presentation: オーストラリア、シドニー
  • Invited