| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, using vanadium-accumulating ascidians as a model system, we performed gene knockout experiments using artificial nucleases and examination of the vanadium export pathway by reverse pathway of vanadium accumulation pathway. In addition, in order to examine more deeply on the molecular mechanism, we also performed metagenomic and transcriptomic analyses. We found the correlation between vanadium concentration and microbiome. We also found novel vanadium-binding proteins and vanadium channels that are expected to be involved in vanadium export pathway.
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