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Attempt of identification and characterization of novel bioactive peptides using the new extraction method

Research Project

Project/Area Number 25440171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionHiroshima University

Principal Investigator

Iwakoshi Eiko  広島大学, 総合科学研究科, 研究員 (50311296)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords生理活性ペプチド / 鳥類
Outline of Final Research Achievements

For the extraction of novel bioactive peptides, ordinary and routine methods using water-extraction combined with acid have been established and employed so far on animal tissues. In the present study, I sought novel bioactive substances in avian and mammalian tissues and tested several organic solvents for the efficient extraction. It was revealed that some bioactive peptides with hydrophobic properties which have been known in the previous studies were identified using dimethyl sulfoxide (DMSO), although I could not find a novel bioactive substance. I found that a novel extraction method using DMSO is suitable for the identification of highly hydrophobic substances.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 3 results,  Open Access: 5 results) Presentation (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Synthesis of neurosecretory protein GM composed of 88 amino acid residues by native chemical ligation2016

    • Author(s)
      Masuda K, Furumitsu M, Ooyama H, Iwakoshi-Ukena E, Ukena K
    • Journal Title

      Tetrahedron Letters

      Volume: 57 Issue: 7 Pages: 804-807

    • DOI

      10.1016/j.tetlet.2016.01.027

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification and localization of gonadotropin-inhibitory hormone (GnIH) orthologs in the hypothalamus of the red-eared slider turtle, Trachemys scripta elegans.2016

    • Author(s)
      Ukena K, Iwakoshi-Ukena E, Osugi T, Tsutsui K
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 227 Pages: 69-76

    • DOI

      10.1016/j.ygcen.2015.06.009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of neurosecretory protein GM using Escherichia coli and Chinese Hamster Ovary cells2015

    • Author(s)
      Masuda K, Furumitsu M, Taniuchi S, Iwakoshi-Ukena E, Ukena K
    • Journal Title

      FEBS Open Bio

      Volume: 5 Issue: 1 Pages: 844-851

    • DOI

      10.1016/j.fob.2015.10.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microwave-assisted solid-phase peptide synthesis of neurosecretory protein GL composed of 80 amino acid residues.2015

    • Author(s)
      Masuda K, Ooyama H, Shikano K, Kondo K, Furumitsu M, Iwakoshi-Ukena E, Ukena K.
    • Journal Title

      Jornal of Peptide Science

      Volume: in press Issue: 6 Pages: 454-460

    • DOI

      10.1002/psc.2756

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of neurotensin and LANT-6 and localization of mRNA encoding their precursor in the chicken brain.2014

    • Author(s)
      Masuda K, Iwakoshi-Ukena E, Bessho Y, Taniuchi S, Maejima S, Shikano K, Kondo K, Furumitsu M, Ukena K.
    • Journal Title

      Zoolical Science

      Volume: 31 Issue: 6 Pages: 353-359

    • DOI

      10.2108/zs140010

    • Related Report
      2015 Annual Research Report 2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain.2014

    • Author(s)
      Bessho Y, Iwakoshi-Ukena E, Tachibana T, Maejima S, Taniuchi S, Masuda K, Shikano K, Kondo K, Furumitsu M, Ukena K.
    • Journal Title

      Neuroscience Letter

      Volume: 578 Pages: 106-110

    • DOI

      10.1016/j.neulet.2014.06.048

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a cDNA encoding a novel small secretory protein, neurosecretory protein GL, in the chicken hypothalamic infundibulum.2014

    • Author(s)
      Ukena K, Iwakoshi-Ukena E, Taniuchi S, Bessho Y, Maejima S, Masuda K, Shikano K, Kondo K, Furumitsu M, Tachibana T.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 446 Issue: 1 Pages: 298-303

    • DOI

      10.1016/j.bbrc.2014.02.090

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ニワトリにおいて視床下部漏斗部に特異的発現を示す新規遺伝子の同定2013

    • Author(s)
      岩越栄子、橘 哲也、谷内秀輔、古満芽久美、益田恵子、浮穴和義
    • Journal Title

      日本家禽学会誌

      Volume: 50

    • NAID

      10031167911

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ニワトリの脳におけるニューロテンシンの同定とmRNA発現解析

    • Author(s)
      益田恵子、岩越‐浮穴栄子、別所裕紀、浮穴和義
    • Organizer
      第65回日本動物学会中国四国支部大会
    • Place of Presentation
      徳島
    • Related Report
      2013 Research-status Report
  • [Presentation] ニワトリの脳からのニューロテンシン及びニューロメディンNの同定

    • Author(s)
      益田恵子、岩越‐浮穴栄子、別所裕紀、浮穴和義
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Remarks] 浮穴研究室のホームページ

    • URL

      http://home.hiroshima-u.ac.jp/ukena/

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 成長ホルモン発現抑制剤、成人成長ホルモン分泌不全症モデル非ヒト動物、成人成長ホルモン分泌不全症モデル非ヒト動物の作製方法、褐色脂肪細胞の機能調整剤、脂肪蓄積症モデル2014

    • Inventor(s)
      浮穴和義、岩越栄子、谷内秀輔、鹿野健史朗、近藤邦裕
    • Industrial Property Rights Holder
      浮穴和義、岩越栄子、谷内秀輔、鹿野健史朗、近藤邦裕
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-10-07
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] ポリペプチド、核酸、発現ベクター、形質転換体、成長ホルモン発現抑制剤、非ヒト動物及び非ヒト動物の作製方法2013

    • Inventor(s)
      浮穴和義、岩越栄子、谷内秀輔、鹿野健史朗、近藤邦裕
    • Industrial Property Rights Holder
      浮穴和義、岩越栄子、谷内秀輔、鹿野健史朗、近藤邦裕
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-10-22
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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