Molecular evolutionary study on a novel pheromone receptor gene common in most vertebrates from fish to mammals

• Project/Area Number: 25440189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Nikaido Masato 東京工業大学, 生命理工学研究科, 准教授 (70432010)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: フェロモン受容体 / 嗅上皮 / 鋤鼻上皮 / 脊椎動物 / ancV1R / 進化 / V1R / 鋤鼻器官

Outline of Final Research Achievements

We recently found one pheromone receptor gene, which is common in most vertebrates (named it ancV1R). To understand the evolutionary origin and the contribution of ancV1R receptor in reproductive behavior of vertebrates, we analyzed this receptor gene from several aspects. First, mRNA of ancV1R gene was shown to be expressed in the entire region of the vomeronasal organ. Furthermore, the pseudonization of ancV1R gene was apparently correlated with the degeneration of vomeronasal organ in most cases we investigated. The above results imply that the ancV1R has the central role in pheromone detection through vomeronasal sensory neurons. We also succeeded in the expression of ancV1R in cultured cell and in the construction of ancV1R-knock-out mice. These experimental systems will be used for the future functional elucidation.

Research Products

• [Journal Article] 生きた化石シーラカンス (2016)
  • Author(s): 二階堂雅人
  • Journal Title: 『Re』 建築保全センター Volume: 189 Pages: 58-59
• [Journal Article] A microsatellite-based genetic linkage map and putative sex-determining genomic regions in Lake Victoria cichlids. (2015)
  • Author(s): Kudo Y., Nikaido M., Kondo A., Suzuki H., Yoshida K., Kikuchi K., Okada N.
  • Journal Title: Gene Volume: 560 Issue: 2 Pages: 156-164
  • DOI: 10.1016/j.gene.2015.01.057
  • Peer Reviewed / Open Access
• [Journal Article] Distinct functions of two olfactory marker protein genes derived from teleost-specific whole genome duplication. (2015)
  • Author(s): Suzuki H, Nikaido M, Yamagishi K, Okada N
  • Journal Title: BMC Evolutionary Biology Volume: 15 Issue: 1 Pages: 245-245
  • DOI: 10.1186/s12862-015-0530-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multiple episodic evolution events in V1R receptor genes of East-African cichlids. (2014)
  • Author(s): Nikaido M, Ota T, Hirata T, Satta Y, Saito Y, Aibara M, Mzighani SI, Hagino-Yamagishi K, Sturmbauer C, Okada N.
  • Journal Title: Genome Biology and Evolution Volume: 6 Issue: 5 Pages: 1135-1144
  • DOI: 10.1093/gbe/evu086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] シーラカンスのもう一つの繁殖地：タンザニア (2014)
  • Author(s): 二階堂雅人、岡田典弘
  • Journal Title: 生物の科学遺伝 Volume: 68 Pages: 210-214
• [Journal Article] シーラカンスゲノム進化：進化速度・嗅覚・前適応 (2014)
  • Author(s): 二階堂雅人、岡田典弘
  • Journal Title: 生物の科学遺伝 Volume: 68 Pages: 256-260
• [Journal Article] シーラカンスゲノム中に隠されていた脊椎動物陸上化のカギ (2014)
  • Author(s): 二階堂雅人、岡田典弘
  • Journal Title: バイオサイエンスとバイオインダストリー Volume: 72 Pages: 134-136
  • NAID: 40020020658
• [Journal Article] Coelacanth genomes reveal a variety of signatures for evolutionary transition from water to land (2013)
  • Author(s): Nikaido M, Noguchi, H, Nishihara H, Toyoda A, Suzuki Y, Kajitani R, Suzuki H, Okuno M, Aibara M, Ngatunga BP, Mzighani SI, Kalombo HW, Masengi KW, Tuda J, Nogami S, Maeda R, Iwata M, Abe Y, Fujimura K, Okabe M, Amano T, Maeno A, Shiroishi T, Itoh T, Sugano S, Kohara Y, Fujiyama A, Okada N.
  • Journal Title: Genome Research Volume: 23巻10号 Issue: 10 Pages: 1740-1748
  • DOI: 10.1101/gr.158105.113
  • Peer Reviewed
• [Journal Article] シーラカンスの全ゲノムが語る脊椎動物の陸上化 (2013)
  • Author(s): 二階堂雅人
  • Journal Title: natureダイジェスト Volume: 10 Issue: 12 Pages: 28-29
  • DOI: 10.1038/ndigest.2013.131228
• [Presentation] ほぼ全ての脊椎動物に存在する新規フェロモン受容体ancV1R の進化 (2015)
  • Author(s): 二階堂雅人
  • Organizer: 第３回ケモビ研究会
  • Place of Presentation: 箱根
  • Year and Date: 2015-11-14
• [Presentation] 全ての脊椎動物に共通な新規フェロモン受容体の機能解明 (2015)
  • Author(s): 鈴木彦有、西田秀史、廣田順二
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟大学
  • Year and Date: 2015-09-17
• [Presentation] 脊椎動物に保存された新規V1R 遺伝子の機能と進化 (2014)
  • Author(s): 鈴木彦有、二階堂雅人
  • Organizer: 第2回ケモビ研究会
  • Place of Presentation: 箱根
  • Year and Date: 2014-10-24 – 2014-10-26
• [Presentation] シクリッドV1Rの多様化と種分化 (2014)
  • Author(s): 二階堂雅人
  • Organizer: 第2回ケモビ研究会
  • Place of Presentation: 箱根
  • Year and Date: 2014-10-24 – 2014-10-26
• [Presentation] 脊椎動物に保存された新規V1R 遺伝子の機能と進化 (2014)
  • Author(s): 鈴木彦有、西田秀史、廣田順二、二階堂雅人
  • Organizer: 第16回日本進化学会
  • Place of Presentation: 高槻・現代劇場
  • Year and Date: 2014-08-21
• [Presentation] Evolution of the chemo-receptor genes in coelacanth. (2014)
  • Author(s): 二階堂雅人、岡田典弘
  • Organizer: 第16回日本進化学会
  • Place of Presentation: 高槻・現代劇場
  • Year and Date: 2014-08-21
  • Invited
• [Presentation] ほぼ全ての脊椎動物に共通な新規V1Rの機能と進化 (2013)
  • Author(s): 鈴木彦有、二階堂雅人
  • Organizer: 第１回 ケモビ研究会
  • Place of Presentation: 箱根
• [Book] 絶滅危惧種のゲノム解読とその利用（次世代シーケンサー活用術） (2015)
  • Author(s): 二階堂雅人、岡田典弘
  • Total Pages: 212
  • Publisher: 化学同人
• [Remarks] 東京工業大学生命理工学院 二階堂研究室ホームページ
  • URL: http://www.nikaido.bio.titech.ac.jp/
• [Remarks] 二階堂雅人のページ
  • URL: https://sites.google.com/site/masatonikaido/