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Elucidation of retinoid acid-dependent mechanism of organogenesis and its evolution in chordates

Research Project

Project/Area Number 25440192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Evolutionary biology
Research InstitutionKochi University

Principal Investigator

Fujiwara Shigeki  高知大学, 教育研究部自然科学系理学部門, 教授 (40229068)

Co-Investigator(Renkei-kenkyūsha) SASAKURA Yasunori  筑波大学, 生命環境系, 教授 (10400649)
NISHINO Atsuo  弘前大学, 農学生命科学部, 准教授 (50343116)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsレチノイン酸 / ホヤ / 脊索動物 / 進化 / Hox1 / レチノイン酸合成酵素Raldh2 / レチノイン酸分解酵素Cyp26 / 転写調節 / 遺伝子発現制御 / レチノイン酸合成酵素 / レチノイン酸分解酵素 / オタマボヤ / 遺伝子発現調節
Outline of Final Research Achievements

Retinoic acid is a signaling molecule important for the chordate development. This study identified region-specific enhancer elements of the genes encoding retinoic acid-synthesizing and degrading enzymes (Raldh2 and Cyp26 respectively) in ascidians. Dominant negative experiments suggested that the transcription factor Mesp activates the Raldh2 enhancer.
Although the body plan of larvaceans is similar to that of ascidians, they completely lack biological pathways to synthesize retinoic acid. Retinoic acid is required for transactivation of Hox1 gene in ascidians but not in larvaceans. The present study revealed that an enhancer of larvacean Hox1 can be activated in ascidian embryos. The results suggest that a retinoic acid-independent mechanism of transactivation of Hox1 has been equipped in the common ancestor of ascidians and larvaceans. This mechanism may have enabled the larvaceans to survive the loss of retinoic acid-synthesizing pathways during their evolution.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] バルセロナ大学(スペイン)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] アリゾナ大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] ANISEED 2015: a digital framework for the comparative developmental biology of ascidians.2015

    • Author(s)
      Brozovic, Martin, Dantec, Dauga, Mendez, Simion, Percher, Laporte, Scornavacca, Di Gregorio, Fujiwara, et al.
    • Journal Title

      Nucleic Acids Research

      Volume: 44 Issue: D1 Pages: 808-818

    • DOI

      10.1093/nar/gkv966

    • NAID

      120007129589

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chondroitin 4-O-sulfotransferases are required for cell adhesion and morphogenesis in the Ciona intestinalis embryo.2015

    • Author(s)
      Nakamura, J., Tetsukawa, A. & Fujiwara, S.
    • Journal Title

      Dev. Growth Differ.

      Volume: 57 Issue: 1 Pages: 58-67

    • DOI

      10.1111/dgd.12188

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Chondroitin 6-O-sulfotransferases are required for morphogenesis of the notochord in the ascidian embryo.2014

    • Author(s)
      Nakamura, J., Yoshida, K., Sasakura, Y. & Fujiwara, S.
    • Journal Title

      Dev. Dyn.

      Volume: 243 Issue: 12 Pages: 1637-1645

    • DOI

      10.1002/dvdy.24213

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Two arginine kinases of Tetrahymena pyriformis: characterization and localization.2014

    • Author(s)
      Michibata, J., Okazaki, N., Motomura, S., Uda, K., Fujiwara, S. and Suzuki, T.
    • Journal Title

      Comp. Biochem. Physiol. Part B.

      Volume: 171 Pages: 34-41

    • DOI

      10.1016/j.cbpb.2014.03.008

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Transcriptional regulation in the early ectodermal lineage of ascidian embryos.2013

    • Author(s)
      Horikawa, Y., Matsumoto, H., Yamaguchi, F., Ishida, S. and Fujiwara, S.
    • Journal Title

      Develop. Growth Differ.

      Volume: 55 Issue: 9 Pages: 776-785

    • DOI

      10.1111/dgd.12100

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Retinoid X receptor-mediated transdifferentiation cascade in budding tunicates.2013

    • Author(s)
      Kawamura, K., Shiohara, M., Kanda, M. and Fujiwara, S.
    • Journal Title

      Dev. Biol.

      Volume: 384 Issue: 2 Pages: 343-355

    • DOI

      10.1016/j.ydbio.2013.10.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a retinoic acid-responsive neural enhancer in the Ciona intestinalis Hoxl gene.2013

    • Author(s)
      Kanda M, Ikeda T, Fujiwara S.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 55(2) Issue: 2 Pages: 260-269

    • DOI

      10.1111/dgd.12033

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Transcriptional regulation of the retinoic acid-synthesizing and degrading enzymes in the Ciona intestinalis embryo2015

    • Author(s)
      Hatakeyama, Y., Taagashira, A., Moriyama, Y., Nabeshima, M., Fujiwara, S.
    • Organizer
      The 8th International Tunicate Meeting
    • Place of Presentation
      リンクステーションホール青森(青森県青森市)
    • Year and Date
      2015-07-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ホヤ胚の遺伝子発現に対する有機スズの影響2014

    • Author(s)
      愛甲由紀,山田友香里,安住薫,藤原滋樹
    • Organizer
      ホヤ研究集会 2014
    • Place of Presentation
      東京都,筑波大学東京キャンパス
    • Year and Date
      2014-10-13
    • Related Report
      2014 Research-status Report
  • [Presentation] ホヤとオタマボヤにおけるHox1 転写調節機構の比較解析2014

    • Author(s)
      田川泉実,神田美幸,Adriana Rodriguez-Mari,Cristian Canestro,藤原滋樹
    • Organizer
      ホヤ研究集会 2014
    • Place of Presentation
      東京都,筑波大学東京キャンパス
    • Year and Date
      2014-10-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Role of glycosaminoglycan sulfotransferases in the ascidian embryogenesis2013

    • Author(s)
      Nakamura, J. and Fujiwara, S.
    • Organizer
      The 7th International Tunicate Meeting
    • Place of Presentation
      イタリア,ナポリ Parthenope 大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Transcriptional regulation in the early ectodermal lineage of ascidian embryo2013

    • Author(s)
      Matsumoto, H., Yamaguchi, F., Horikawa, Y., Ishida, S. and Fujiwara, S.
    • Organizer
      The 7th International Tunicate Meeting
    • Place of Presentation
      イタリア,ナポリ Parthenope 大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Gene loss impact on EvoDevo: dismantling the retinoic acid genetic machinery in Oikopleura dioica2013

    • Author(s)
      Martí-Solans, J., Beliaeva, O., Kanda, M., Badia-Ramentol, J., Godoy-Marin, H., Postlethwait, J. H., Fujiwara, S., Chourrout, D., Albalat, R. and Cañestro, C.
    • Organizer
      The 7th International Tunicate Meeting
    • Place of Presentation
      イタリア,ナポリ Parthenope 大学
    • Related Report
      2013 Research-status Report
  • [Presentation] カタユウレイボヤにおけるmiR-124の発現機構2013

    • Author(s)
      松崎信生・西岡雅都・藤原滋樹
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山市,岡山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 尾索動物のセルロース合成酵素の示す組織特異的発現の仕組み2013

    • Author(s)
      笹倉靖徳・小椋陽介・横森類・朴聖俊・中井謙太・藤原滋樹・吉田慶太
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山市,岡山大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2022-01-27  

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