Mechanism for production of starch-like reserve polysaccharide in some cyanobacteria
| Project/Area Number |
25440193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
Suzuki Eiji 秋田県立大学, 生物資源科学部, 教授 (60211984)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 貯蔵多糖 / 澱粉 / グリコーゲン / 光合成 / 原核生物 / 細菌 / シアノバクテリア / 進化 / アミロペクチン / Cyanothece |
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| Outline of Final Research Achievements |
The present study was conducted to elucidate the mechanism by which a limited number of cyanobacteria (photosynthetic microorganisms) produce starch-like reserve polysaccharide seen in plants instead of glycogen in most bacteria. Genomic sequences were determined for two starch-producing strains, and the starch production was found to be tightly correlated to the coordinated expression of multiple (2 - 3) isoforms of branching enzyme. Heterologous expression system for starch metabolism genes (starch synthase I and branching enzyme I of rice, and debranching enzyme glgX2 of Cyanobacterium CLg1) was developed in glycogen-producing cyanobacterial strais. Growth conditions were devised to stimulate starch production under deprivation of inorganic nutrients. Finally, recombinant forms of branching enzyme were characterized; form 1 and 2 branching enzymes transferred short oligosaccharides, while the activity of form 3 enzyme was low but it transferred short and long glucan chains.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Diversity of reaction characteristics of glucan branching enzymes and the fine structure of α-glucan from various sources.2014
Author(s)
Sawada, T., Y. Nakamura, T. Ohdan, A. Saitoh, Francisco PB Jr, E. Suzuki, N. Fujita, T. Shimonaga, S. Fujiwara, M. Tsuzuki, C. Colleoni, S. Ball
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Journal Title
Arch. Biochem. Biophys.
Volume: 562
Pages: 9-21
DOI
Related Report
Peer Reviewed
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[Journal Article] Convergent evolution of polysaccharide debranching defines a common mechanism for starch accumulation in cyanobacteria and plants2013
Author(s)
Cenci U, Chabi M, Ducatez M, Tirtiaux C, Nirmal-Raj J, Utsumi Y, Kobayashi D, Sasaki S, Suzuki E, Nakamura Y, Putaux J-L, Roussel X, Durand-Terrasson A, Bhattacharya D, Vercoutter-Edouart A-S, Maes E, Arias MC, Palcic M, Sim L, Ball SG, Colleoni C
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Journal Title
Plant Cell
Volume: 25
Issue: 10
Pages: 3961-3975
DOI
Related Report
Peer Reviewed
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[Presentation] 澱粉生産性シアノバクテリア由来枝切り酵素の機能解析2015
Author(s)
木村友亮, 鈴木龍一郎, Christophe Colleoni, Steven G. Ball, 藤田直子, 鈴木英治
Organizer
日本応用糖質科学会平成27年度大会（第64回）
Place of Presentation
奈良春日野国際フォーラム（奈良市）
Year and Date
2015-09-16
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