| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The present study was conducted to elucidate the mechanism by which a limited number of cyanobacteria (photosynthetic microorganisms) produce starch-like reserve polysaccharide seen in plants instead of glycogen in most bacteria. Genomic sequences were determined for two starch-producing strains, and the starch production was found to be tightly correlated to the coordinated expression of multiple (2 - 3) isoforms of branching enzyme. Heterologous expression system for starch metabolism genes (starch synthase I and branching enzyme I of rice, and debranching enzyme glgX2 of Cyanobacterium CLg1) was developed in glycogen-producing cyanobacterial strais. Growth conditions were devised to stimulate starch production under deprivation of inorganic nutrients. Finally, recombinant forms of branching enzyme were characterized; form 1 and 2 branching enzymes transferred short oligosaccharides, while the activity of form 3 enzyme was low but it transferred short and long glucan chains.
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