Evolvability of thermophilic proteins from archaea and bacteria

• Project/Area Number: 25440194
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Takano Kazufumi 京都府立大学, 生命環境科学研究科(系), 教授 (40346185)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タンパク質 / 進化 / 安定性 / 好熱菌

Outline of Final Research Achievements

To understand the evolution of life, we focused proteins. Proteins have changed their amino acid sequences along with evolution. In this study, we investigated a random mutational drift of proteins for evolution. Our results collectively support a crucial link between protein evolution and stabilization mechanisms based on the evolutionary history of organisms.

Research Products

• [Journal Article] Evolvability of thermophilic proteins from archaea and bacteria (2013)
  • Author(s): Takano, K., Aoi, A., Koga, Y., and Kanaya, S.
  • Journal Title: Biochemistry Volume: 52 Issue: 28 Pages: 4774-4780
  • DOI: 10.1021/bi400652c
  • Peer Reviewed
• [Presentation] 好熱性Bacteria由来タンパク質の進化過程における安定性の役割 (2016)
  • Author(s): 太田夏子、倉橋亮、佐野智、高野和文
  • Organizer: 第１６回日本蛋白質科学会
  • Place of Presentation: 福岡
  • Year and Date: 2016-06-07
• [Presentation] 生物進化過程におけるタンパク質の安定性の役割 (2016)
  • Author(s): 倉橋亮、佐野智、高野和文
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-27
• [Presentation] 好熱性Archaeaタンパク質の進化過程における安定性と活性の関係 (2015)
  • Author(s): 倉橋亮、佐野智、高野和文
  • Organizer: 日本農芸化学会関西支部第489回例会
  • Place of Presentation: 京都
  • Year and Date: 2015-05-23
• [Presentation] 好熱性タンパク質のEvolvabilityと安定性の関係 (2014)
  • Author(s): 倉橋亮、岡田淳、佐野智、高野和文
  • Organizer: 第１４回日本蛋白質科学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] 好熱性ArchaeaとBacteria由来タンパク質のEvolvability (2013)
  • Author(s): 高野和文、青井敦史、古賀雄一、金谷茂則
  • Organizer: 第１５回日本進化学会
  • Place of Presentation: つくば
• [Presentation] Evolvability of thermophilic proteins from archaea and bacteria (2013)
  • Author(s): K. Takano, A. Aoi, Y. Koga, S. Kanaya
  • Organizer: Thermophiles 2013
  • Place of Presentation: Regensburg