Phylogeny and ecophysiology of fungi possessing ligninolytic activity under snow cover.
Project/Area Number |
25440198
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biodiversity/Systematics
|
Research Institution | Hokkaido University |
Principal Investigator |
MIYAMOTO Toshizumi 北海道大学, (連合)農学研究科(研究院), 講師 (00343012)
|
Co-Investigator(Kenkyū-buntansha) |
KODA Keiichi 北海道大学, 大学院農学研究院, 講師 (80322840)
SHIGETOMI Kengo 北海道大学, 大学院農学研究院, 助教 (20547202)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 積雪下 / 落葉 / 分解 / 菌類 / リグニン / 分子系統 / 酵素 / 積雪 |
Outline of Final Research Achievements |
We isolated litter-decomposing fungi capable of producing active extracellular phenoloxidases at 0 °C from decomposing litter under snow cover in the forests. Molecular phylogenetic analysis and identification of extracellular enzymes of these fungi were conducted. It was revealed that the fungi involved in lignin decomposition under snow cover were consisted of various taxonomic groups inhabiting in litter of various plant species. These fungi were isolated from beginning of snow cover period to snow melting season. Lac, LiP, and MnP active at 4°C were produced from the mycelia of these fungi. A new method to determine lignin content of decomposing leaf litter was also developed. Loss of lignin in litter during the snow cover period was monitored by the new method. Lignin was lost at higher rate than previously reported.
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Report
(4 results)
Research Products
(2 results)