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The function of mating pheromone and the speciation in Blepharisma

Research Project

Project/Area Number 25440210
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biodiversity/Systematics
Research InstitutionNara Women's University

Principal Investigator

Harumoto Terue  奈良女子大学, 自然科学系, 教授 (80198936)

Co-Investigator(Kenkyū-buntansha) 洲崎 敏伸  神戸大学, 理学研究科, 准教授 (00187692)
飯尾 英夫  大阪市立大学, 大学院理学研究科, 教授 (80145771)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords交配フェロモン / 種分化 / 系統樹 / アルベオラータ / 原生生物 / 接合 / 交配型 / 有性生殖 / 性フェロモン
Outline of Final Research Achievements

To investigate the function and the action mechanism of sex pheromone (gamone) in ciliate Blepharisma, ①Isolated gamone 1 genes from several stocks of Blepharisma and identified the sites which determine the specificity of species, ② examined the localization of gamone receptors in the cells, ③ tried to identify genes involved in biosynthesis of gamone 2. In order to clarify the phylogenetic relationship of the strains of Blepharisma, ④ isolated several genes and constructed phylogenetic trees, and ⑤ investigated the specificity of gamone 1 between species, whether the pair formation between different megakaryotype strains and between different species occrred, and whether progenies between such crosses survived, and reexamined the classification of the genus Blepharisma.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (44 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 5 results) Presentation (34 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Macerata(Italy)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Rapid response to nutrient depletion on the expression of mating pheromone, gamone 1, in Blepharisma japonicum2016

    • Author(s)
      Sugiura M, Yamanaka M、Suzaki T, Harumoto T
    • Journal Title

      J.J.Protozoology

      Volume: 49 Pages: 27-36

    • NAID

      130005249727

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dielectric measurement of Euglena gracilis as a multi-parametric approach for non-invasive biomonitoring of aquatic environment.2016

    • Author(s)
      Chen Lin, Ma Qing, Toshinobu Suzaki
    • Journal Title

      Int. J. Environ. Agr. Res.

      Volume: 2 Pages: 59-64

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Use of ionic liquid for scanning electron microscopy of protists.2016

    • Author(s)
      Hideki Ishida, Yukari Gobara, Mayumi Kobayashi, Toshinobu Suzaki
    • Journal Title

      Int. J. New Tech. Res.

      Volume: 12 Pages: 43-46

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rapid response to nutrient depletion on the expression of mating pheromone, gamone 1, in Blepharisma japonicum.2016

    • Author(s)
      Sugiura M, Yamanaka M, Suzaki T and Harumoto T
    • Journal Title

      Jpn. J. Protozool.

      Volume: 未定

    • NAID

      130005249727

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Recent Application of Electron Tomography in Biology.2016

    • Author(s)
      Song, C., Jung, H.S. and Suzaki, T.
    • Journal Title

      J. Electr. Microsc. Technol. Med. Biol.

      Volume: 29 Pages: 97-98

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tight binding of NAP-22 with acidic membrane lipids2015

    • Author(s)
      Maekawa, S., Kobayashi, Y., Morita, M. and Suzaki, T.
    • Journal Title

      Neurosci Lett.

      Volume: 600 Pages: 244-248

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Two possible barriers blocking conjugation between different megakaryotypes of Blepharisma2015

    • Author(s)
      Mayumi Kobayashi, Mamiko Miura, Mari Takusagawa, Mayumi Sugiura, and Terue Harumoto
    • Journal Title

      Zoological Science

      Volume: 32 (1) Issue: 1 Pages: 53-61

    • DOI

      10.2108/zs140151

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Single amino acid substitution alters omnipotent eRF1 of Dileptus to Euplotes-type dualpotent eRF1: standard codon usage may be advantageous in raptorial ciliates.2013

    • Author(s)
      Li Y, Kim, OTP, Ito K, Saito K, Suzaki T, and Harumoto T
    • Journal Title

      Protist

      Volume: 164 Pages: 440-449

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The defensive function of trichocysts in Paramecium tetraurelia against metazoan predators compared with the chemical defense of two species of toxin-containing ciliates.2013

    • Author(s)
      Buonanno F, Harumoto T, and Ortenzi C
    • Journal Title

      Zoological Science

      Volume: 30 Pages: 255-261

    • NAID

      40019626238

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 繊毛虫Loxodesから得られた共生藻株について.2017

    • Author(s)
      保科 亮・早川昌志・洲崎敏伸
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 混合栄養性ユーグレノイドRapaza viridisによるTetraselmis葉緑体の“分割”利用.2017

    • Author(s)
      丸山 萌,洲崎 敏伸,柏山 祐一郎
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 渦鞭毛藻類Oxyrrhis marinaの染色体の3D構造解析.2017

    • Author(s)
      洲崎敏伸・福田康弘・ソンチホン・村田和義
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 有中心粒太陽虫ハリタイヨウチュウの細胞外被殻の構造と形成機構2017

    • Author(s)
      千原あかね・洲崎敏伸
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 化学物質に対するユーグレナの誘電性質の解析2017

    • Author(s)
      陳林・洲崎敏伸
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミドリゾウリムシに共生するChlorella variabilisの細胞壁の解析2017

    • Author(s)
      樋口里樹,ソンチホン,洲崎敏伸
    • Organizer
      第41回日本藻類学会大会
    • Place of Presentation
      高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミカンキジラミ共生細菌の微細構造2016

    • Author(s)
      洲崎敏伸・ソンチホン・村田和義・中鉢 淳
    • Organizer
      生理研ワークショップ「電子顕微鏡ビッグデータが拓くバイオメディカルサイエンス」
    • Place of Presentation
      岡崎市
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3-dimentional analysis of dinoflagellate nucleus by electron microscopy2016

    • Author(s)
      Fukuda, Y. , Suzaki, T. , Mori, A. , Song, C. , Murata, K.
    • Organizer
      生理研ワークショップ「電子顕微鏡ビッグデータが拓くバイオメディカルサイエンス」(岡崎市)
    • Place of Presentation
      岡崎市
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 原生生物の環境センシングと運動2016

    • Author(s)
      上田 昌宏・稲葉 一男・園部 誠司・中垣 俊之・洲崎敏伸
    • Organizer
      第31回日本微生物生態学会
    • Place of Presentation
      横須賀市
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Blepharisma hyalinumの表層顆粒の機能の探索2016

    • Author(s)
      田村望・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 繊毛虫Blepharismaの異なるmegakaryotype間での異種間接合2016

    • Author(s)
      杉野亜優・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 繊毛虫ブレファリズマにおいてガモン2生合成に関与する新規IDOの同定2016

    • Author(s)
      杉浦真由美・湯浅創・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミドリゾウリムシへ共生するクロレラの細胞壁の微細構造と化学組成の変化2016

    • Author(s)
      樋口里樹・ソンチホン・洲崎 敏伸
    • Organizer
      第49回日本原生生物学会大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ハリタイヨウチュウにおけるβ-1.3-グルカンを介した餌の認識に関わる分子の探索2016

    • Author(s)
      小林真弓・Mousumi Bhadra・洲崎敏伸
    • Organizer
      第49回日本原生生物学会大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Managing the phototoxity of chlorophrylls: a basic study on a key metabolism responsible for the mass culture crush2016

    • Author(s)
      Yuichiro Kashiyama, Moe Maruyama, Motoki Kayama, Masami Nakazawa, Takahiro Ishikawa, Toshinobu Suzaki, Tamiaki, Hitoshi
    • Organizer
      11th International Marine Biotechnology Conference IMBC 2016
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An evolutionary transition of chloroplast degradation in euglenoids: Heterotrophic digestion to secondary plasmid senescence2016

    • Author(s)
      Kashiyama Y. , Kawahara J. , Maruyama Nakazawa M. , Yokoyama A., Ishikawa T. , Tamiaki H., Suzaki T. M. , Kayama M. ,
    • Organizer
      Protist 2016
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chloroplast division of Tetraselmis sp. in the food vacuole of mixotrophic algae Rapaza viridis2016

    • Author(s)
      Maruyama M., Miyagishima S, Suzaki T. , Kashiyama Y.
    • Organizer
      Protist 2016
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ocher-colored granules in the ciliate Cyclotrichium sp. are evidently diatom chloroplasts2016

    • Author(s)
      Hoshina, R., Suzaki, T. , Kusuoka, Y.
    • Organizer
      Protist 2016
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Improved preservation of organelles in Paramecium bursaria by freeze-substitution with glutaraldehyde and osmium tetroxide.2016

    • Author(s)
      ソンチホン・洲崎敏伸
    • Organizer
      医学生物学電子顕微鏡技術学会第32回学術講演会
    • Place of Presentation
      東京都
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 原生動物ハリタイヨウチュウを用いた高感度・高速な水質バイオモニタリングシステムの開発2016

    • Author(s)
      吉村知里・洲崎敏伸
    • Organizer
      環境科学会2016年会
    • Place of Presentation
      横浜市
    • Related Report
      2016 Annual Research Report
  • [Presentation] ブレファリズマの性成熟過程における接合型発現パターンと遺伝子発現解析2015

    • Author(s)
      杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所 東京
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] Blepharisma hyalinum の分類の再検討2015

    • Author(s)
      小林真弓・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所 東京
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] ブレファリズマのジャイアント形成の意義と形成条件の検討2015

    • Author(s)
      尾野優奈・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所 東京
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 繊毛虫ブレファリズマにおける交配フェロモンの多様性と種分化2015

    • Author(s)
      春本晃江・小林真弓・杉浦真由美・篠原きよの・田草川真里
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ 新潟コンベンションセンター
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Mating type expression during sexual maturation in Blepharisma stoltei.2015

    • Author(s)
      Mayumi Sugiura and Terue Harumoto
    • Organizer
      Ciliate Molecular Biology Conference
    • Place of Presentation
      Camerino, Italy
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mating pheromone gamone 1 plays an important role in speciation of the ciliate Blepharisma.2015

    • Author(s)
      Mayumi Kobayashi, Yui Nishihara, Mamiko Miura, Mari Takusagawa, Mayumi Sugiura, Terue Harumoto
    • Organizer
      Ciliate Molecular Biology Conference
    • Place of Presentation
      Camerino, Italy
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 繊毛虫Blepharisma属における交配フェロモンgamone1の多様性と種分化2014

    • Author(s)
      小林真弓, 田草川真理, 杉浦真由美, 春本晃江
    • Organizer
      日本原生生物学会第47回大会
    • Place of Presentation
      仙台市宮城教育大学
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] ブレファリズマの交配フェロモン(ガモン1)の糖鎖構造とその役割2014

    • Author(s)
      春本晃江, 山岸由和, 岩﨑祥子, 杉浦真由美, 小林真弓, 飯尾英夫
    • Organizer
      日本原生生物学会第47回大会
    • Place of Presentation
      仙台市宮城教育大学
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 繊毛虫ブレファリズマ属の分類の再検討2014

    • Author(s)
      小林真弓, 杉浦真由美, 春本晃江
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台市東北大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 繊毛虫ブレファリズマの異種間接合を妨げる要因2013

    • Author(s)
      小林真弓、杉浦真由美、春本晃江
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 接合誘導物質ガモン2 に類似したアミノ酸が繊毛虫Blepharisma のガモン2 活性に与える影響2013

    • Author(s)
      篠原きよの、春本晃江、杉浦真由美
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞密度がブレファリズマのジャイアント形成に与える影響2013

    • Author(s)
      尾野優奈、杉浦真由美、春本晃江
    • Organizer
      日本原生動物学会第46回大会
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 繊毛虫Blepharismaの異種間接合対において核変化は起こりうるのか2013

    • Author(s)
      山田 真央、小林 真弓、杉浦 真由美、春本 晃江
    • Organizer
      日本原生動物学会第46回大会
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Two barriers prevent interspecific mating pair formation in Blepharisma.2013

    • Author(s)
      Kobayashi, M. Sugiura M and Harumoto T
    • Organizer
      FASEB Science Research Conferences: Ciliate Molecular Biology
    • Place of Presentation
      Steamboat Springs, USA
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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