| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of this study was to classify Pestalotiopsis into genera using its sexual morph and mating concept. Pestalotiopsis s. l. was separated into three genera in 2014 and assigned to the Pestalotiopsaceae family in 2015. For these 3 genera, 13 strains that produce sexual morph have been reported only in Pestalotiosis s. st. In this study, we found two strains in Pestalotiopsis s. l. that produce sexual morph. Because the HMG-box of the MAT1-2 genes of this genera did not amplify using universal primers for ascomycetes, we analyzed NGS data of these sexual morph strains and three asexual strains. The MAT1-2 genes differed in the number of nucleotides and the position of the HMG-box in each strain. Therefore, full-length of MAT1-2 gene is not suitable for phylogenetic study among these genera. Based on HMG-box sequences and continued analysis of all the genomes, we infer that Pestalotiopsis s. l. may separate into two genera.
|
