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Experimental analysis of an early stage of endosymbiosis between an alga and a protozoon using a synthetic microbial ecosystem

Research Project

Project/Area Number 25440240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionEhime University

Principal Investigator

Nakajima Toshiyuki  愛媛大学, 理工学研究科, 教授 (70314945)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords細胞内共生 / マイクロコズム / 実験進化 / 進化 / テトラヒメナ / クロレラ / ミクラクティニウム
Outline of Final Research Achievements

Endo-symbiosis is widely recognized as a process generating evolutionary innovation, in which unicellular organisms of a particular species live within, i.e., metabolize and propagate synchronically with a host cell of a different species. We conducted an unprecedented experiment of a long-term culture of a green alga (Micractinium sp.), a bacterium (E. coli), and a ciliate (Tetrahymena theormophila), in order to observe directly the evolution of component species in the culture. In this study, we revealed that a primitive form of endo-symbiosis between the alga and the ciliate, and also ecto-symbiosis between the alga and the bacterium, evolved in the long-term culture.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Biologically inspired information theory: Adaptation through construction of external reality models by living systems2015

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Progress in Biophysics and Molecular biology

      Volume: 119 Issue: 3 Pages: 634-648

    • DOI

      10.1016/j.pbiomolbio.2015.07.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of a free-living alga into forms with ecto- and endosymbiotic associations with heterotrophic organisms in a 5-year microcosm culture2015

    • Author(s)
      Toshiyuki Nakajima, Yoshiyuki Fujikawa, Toshiyuki Matsubara, Mimi Karita, Akiko Sano
    • Journal Title

      BioSystems

      Volume: 131 Pages: 9-21

    • DOI

      10.1016/j.biosystems.2015.03.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of acidic conditions on the physiology of a green alga (Micractinium sp.) before and after a 5-year interaction with Tetrahymena thermophila in an experimental microcosm2014

    • Author(s)
      Arno Germond, Masahiro Inouhe & Toshiyuki Nakajima
    • Journal Title

      European Journal of Phycology

      Volume: 49 Issue: 4 Pages: 526-537

    • DOI

      10.1080/09670262.2014.978393

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 生態系は進化装置:人工生態系の長期培養による解析と展望2015

    • Author(s)
      中島敏幸
    • Organizer
      進化群集生態学シンポジウム
    • Place of Presentation
      京都大学(京都市左京区)
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 現象学的視点からみる生命システム:情報・モデル・進化をめぐって2015

    • Author(s)
      中島敏幸
    • Organizer
      生物学基礎論研究会
    • Place of Presentation
      東京農業大学オホーツクキャンパス(北海道網走市))
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 人工生態系における藻類の分化と共生進化の生態学的機構2015

    • Author(s)
      中島敏幸、藤川佳之、松原俊行
    • Organizer
      日本進化学会
    • Place of Presentation
      中央大学(東京都文京区)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] モデル生態系を用いた藻類と繊毛虫の細胞内共生の進化における選択過程の解析:ホストに利益を与える共生藻の選択機構をさぐる2014

    • Author(s)
      中島 敏幸・松原 俊之・大西 陽一郎
    • Organizer
      日本原生生物学会
    • Place of Presentation
      宮城教育大学(宮城県仙台市青葉区荒巻字青葉)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] モデル生態系における進化過程:藻類と原生動物の細胞内共生2014

    • Author(s)
      大西陽一郎・中島敏幸
    • Organizer
      生物学基礎論研究会
    • Place of Presentation
      愛媛大学(愛媛県松山市文京町)
    • Year and Date
      2014-08-29 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 実験モデル生態系で見る細胞内共生進化の初期過程: 繊毛虫による藻類取込率の変化の解析2014

    • Author(s)
      大西陽一郎・佐野明子・中島敏幸
    • Organizer
      日本進化学会
    • Place of Presentation
      高槻現代劇場(大阪府高槻市野見町)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 長期培養モデル生態系における藻類と細菌の共生関係の進化の解析2014

    • Author(s)
      松浦正幸・藤井陽介・中島敏幸
    • Organizer
      日本進化学会
    • Place of Presentation
      高槻現代劇場(大阪府高槻市野見町)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 3種からなるモデル生態系を用いた藻類と繊毛虫の細胞内共生進化の初期過程の解析2013

    • Author(s)
      中島敏幸
    • Organizer
      日本原生動物学会 46回
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 長期培養モデル生態系における繊毛虫-藻類間の細胞内共生の初期進化における繊毛虫による藻類の取り込み率の変化2013

    • Author(s)
      大西陽一郎、佐野明子、中島敏幸
    • Organizer
      日本原生動物学会 46回
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 長期培養モデル生態系における藻類と細菌の種間関係の進化2013

    • Author(s)
      松浦正幸、藤井陽介、中島敏幸
    • Organizer
      日本原生動物学会 46回
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
  • [Book] Symbiotic Associations in Ciliates: Ecological and Evolutionary Perspectives2016

    • Author(s)
      Arno Germond and Toshiyuki iNakajima
    • Total Pages
      21
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Remarks] 実験生態系における藻類と原生動物間の細胞内共生の進化

    • URL

      http://www.ehime-u.ac.jp/infinity/detail_8901.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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