Searching genomic region participating adaptation to environment

• Project/Area Number: 25440254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical anthropology
• Research Institution: Tohoku University
• Principal Investigator: DANJOH Inaho 東北大学, 東北メディカル・メガバンク機構, 講師 (30415228)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 南米 / 先住民族 / ゲノム / コピー数多型 / SNP / 適応

Outline of Final Research Achievements

Amerinds, indigenous people in South America, are Mongoloid originated from Eurasian continents. During the migration path from Eurasian continent to South American continent approx. twelve thousand years ago, they have adapted to harsh environments; mountainous area height over 40,000 meters, or lowland where people take poisonous crops for staple. These life styles suggest that people have been underwent some adaptive selection. Analysis of genomic polymorphisms relating to environmental adaptations may reveal migration path of ancestors of Amerinds during prehistoric era. As a result of analysis, we identified several candidate SNPs relating to acclimatization to altitude and detoxification.

Research Products

• [Journal Article] GWAS of clinically defined gout and subtypes identifies multiple susceptibility loci that include urate transporter genes. (2017)
  • Author(s): Nakayama A, Nakaoka H, Yamamoto K, Sakiyama M, Shaukat A, Toyoda Y, Okada Y, Ohkawa Y,他39名
  • Journal Title: Ann Rheum Dis. Volume: 76 Issue: 5 Pages: 869-877
  • DOI: 10.1136/annrheumdis-2016-209632
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Monitoring of minimal residual disease in early T-cell precursor acute lymphoblastic leukaemia by next-generation sequencing (2016)
  • Author(s): Pan X, Nariai N, Fukuhara N, Saito S, Sato Y, Katsuoka F, Kojima K, Kuroki Y, Danjoh I, Saito R, Hasegawa S, Okitsu Y, Kondo A, Onishi Y, Nagami F, Kiyomoto H, Hozawa A, Fuse N, Nagasaki M, Shimizu R, Yasuda J, Harigae H, Yamamoto M
  • Journal Title: Br J Haematol Volume: in press Issue: 2 Pages: 318-321
  • DOI: 10.1111/bjh.13948
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The structural origin of metabolic quantitative diversity. (2016)
  • Author(s): Koshiba S, Motoike I, Kojima K, Hasegawa T, Shirota M, Saito T, Saigusa D, Danjoh I, Katsuoka F, Ogishima S, ... Yamamoto M.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 31463-31463
  • DOI: 10.1038/srep31463
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide association study of clinically defined gout identifies multiple risk loci and its association with clinical subtypes. (2015)
  • Author(s): Matsuo H, et al.
  • Journal Title: Ann Rheum Dis. Volume: 印刷中 Issue: 4 Pages: 652-659
  • DOI: 10.1136/annrheumdis-2014-206191
  • Peer Reviewed / Open Access
• [Journal Article] Japonica array: improved genotype impuration by designing a population-specific SNP array with 1070 Japanese individuals (2015)
  • Author(s): Yosuke Kawai, Takahiro Mimori, Kaname Kojima, Naoki Nariai, Inaho Danjoh, Rumiko Saito, Jun Yasuda, Masayuki Yamamoto, and Masao Nagasaki
  • Journal Title: Journal of Human Genetics Volume: 60(10) Issue: 10 Pages: 581-587
  • DOI: 10.1038/jhg.2015.68
  • Peer Reviewed / Open Access
• [Journal Article] Rare variant discovery by deep whole-genome sequencing of 1070 Japanese Individuals (2015)
  • Author(s): Nagasaki M, Yasuda J, Katsuoka F, Nariai N, Kojima K, Kawai Y, Yamaguchi-Kabata Y, Yokozawa J, Danjoh I, Saito S, Sato Y, Mimori T, Tsuda K, Saito R, Pan X, Nishikawa S, Ito S, Kuroki Y, Tanabe O, Fuse N, Kuriyama S, Kiyomoto H, Hozawa A, Minegishi N, Engel J D, Kinoshita K, Kure S, Yaegashi N, Yamamoto M.
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 8018-8018
  • DOI: 10.1038/ncomms9018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-wide association study of clinically-defined gout identifies multiple risk loci and its association with clinical subtypes (2015)
  • Author(s): H Matsuo,K Yamamoto,H Nakaoka,A Nakayama,M Sakiyama,T Chiba,A Takahashi,T Nakamura,H Nakashima,Y Takada,I Danjoh, et al.
  • Journal Title: Annals of the Rheumatic Diseases Volume: in press
  • Peer Reviewed
• [Journal Article] Identification of A Unique Hepatocellular Carcinoma Line, Li-7, With CD13(+) Cancer Stem Cells Hierarchy And Population Change Upon Its Differentiation During Culture And Effects of Sorafenib (2015)
  • Author(s): T Yamada,M Abei,I Danjoh,R Shirota,T Yamashita,I Hyodo,Y Nakamura
  • Journal Title: BMC cancer Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] Validation of multiple single nucleotide variation calls by additional exome analysis with a semiconductor sequencer to supplement data of whole-genome sequencing of a human population (2014)
  • Author(s): Motoike IN, Matsumoto M, Danjoh I, Katsuoka F, Kojima K, Nariai N, Sato Y, Yamaguchi-Kabata Y, Ito S, Kudo H, Nishijima I, Nishikawa S, Pan X, Saito R, Saito S, Saito T, Shirota M, Tsuda K, Yokozawa J, Igarashi K, Minegishi N, Tanabe O, Fuse N, Nagasaki M, Kinoshita K, Yasuda J, and Yamamoto M.
  • Journal Title: BMC Genomics Volume: 10 Issue: 1 Pages: 637-637
  • DOI: 10.1186/1471-2164-15-673
  • Peer Reviewed / Open Access
• [Journal Article] Dominant expansion of a cryptic subclone possessing an abnormal karyotype occurs in B lymphoblastoid cell lines during culture. (2013)
  • Author(s): Inaho Danjoh
  • Journal Title: Cytogenetics and Genome Research Volume: 139 Issue: 2 Pages: 88-96
  • DOI: 10.1159/000343757
  • Peer Reviewed
• [Presentation] Identification of Copy Number Variation in the genome of Japanese population. (2015)
  • Author(s): Inaho.danjoh, Kaoru Tsuda, Natsumi Konno, Rumiko Saito, Jun Yasuda
  • Organizer: The American Society of Human Genetics Annual Meeting
  • Place of Presentation: 米国、Baltimore
  • Year and Date: 2015-10-06
  • Int'l Joint Research
• [Presentation] A whole genome sequencing workflow optimized for a large-scale genome cohort at Tohoku Medical Megabank Organization (ToMMo) (2015)
  • Author(s): Fumiki Katsuoka, Junji Yokozawa, Xiaoquing Pan, Kaoru Tsuda, Inaho Danjo, Rumiko Saito, Sakae Saito, Yoko Kuoroki, Shin Ito, Masao Nagasaki, Jun Yasuda, Masayuki Yamamoto
  • Organizer: 国際ゲノム会議
  • Place of Presentation: 一橋大学（東京都千代田区）
  • Year and Date: 2015-05-20
  • Int'l Joint Research
• [Presentation] Population structure and genetic diversity by genome-wide SNP variation in 19 American ethnic groups scross South America (2014)
  • Author(s): S Nishikawa,R Saito,N Konno,G Tamiya,N Fuse,Y Nakamura,J Yasuda,I Danjoh
  • Organizer: The American Society of Human Genetics Annual Meeting
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2014-10-18 – 2014-10-22
• [Presentation] 園田・田島コレクション細胞のゲノムワイドSNPデータを用いた南米先住民族の集団構造解析 (2013)
  • Author(s): 神澤秀明、久保充明、高橋篤、中村幸夫、檀上稲穂、Timothy A.J.Jinam、齋藤成也
  • Organizer: 日本進化学会
  • Place of Presentation: 茨城県つくば市
• [Presentation] 南米少数民族におけるY-STRハプロタイプのネットワーク解析 (2013)
  • Author(s): 山本敏充、檀上稲穂、中村幸夫
  • Organizer: 日本人類学会
  • Place of Presentation: 茨城県つくば市
• [Presentation] 東北メディカル・メガバンクにおけるシークエンス解析プラットフォームの紹介 (2013)
  • Author(s): 勝岡史城, 横澤潤二, 潘小青, 齋藤さかえ, 津田薫, 檀上稲穂, 齋藤るみ子, 長﨑正朗, 田邉 修, 布施昇男, 安田純, 山本雅之
  • Organizer: 第3回ＮＧＳ現場の会
  • Place of Presentation: 兵庫県神戸市
• [Remarks] 東北メディカル・メガバンク機構 成果一覧
  • URL: http://www.megabank.tohoku.ac.jp/news/category/achievements
• [Remarks] 理化学研究所バイオリソースセンター細胞材料開発室
  • URL: http://www.riken.jp/research/labs/brc/cell_eng/