| Project/Area Number |
25450004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUMURA Hideo 信州大学, 学術研究院繊維学系, 准教授 (40390885)
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| Co-Investigator(Renkei-kenkyūsha) |
URASAKI Naoya 沖縄県農業研究センター, 研究企画班, 上席主任研究員 (20504591)
TARORA Kazuhiko 沖縄県農業研究センター, 研究企画班, 主任研究員 (10504772)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 性決定 / 性染色体 / ゲノム解析 / MADS-box / パパイヤ / MADS box / アグロインフィルトレーション / 遺伝子発現解析 / MADS-boxタンパク質 / DNA多型 |
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| Outline of Final Research Achievements |
Papaya (Carica papaya) is a trioecious plant species, and its sex types were determined by sex chromosomes (X, Y, Yh). In the present study, genome sequences of Y and Yh chromosomes were analyzed and their structural differences were identified. Consequently, only a SVP-like gene, harbouring MADS-box domain, showed apparent structural difference between Y and Yh chromosomes. In its allele on the Yh chromosome, a transposon was inserted and 5' region was truncated in its transcript, resulting in loss of MADS-box domain in its encoded protein. RT-PCR analysis and transient expression study in Nicotiana benthamiana leaves demonstrated that expression product from its allele in Yh chromosome was possibly unstable. Therefore, this SVP-like gene was supposed to be a candidate gene for male/hermaphrodite determination in papaya. Additionally, RNA-seq analysis of X chromosomal genes showed possibility of gene dosage compensation between female and hermaphrodite.
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