| Project/Area Number |
25450006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Science in genetics and breeding
|
|---|
| Research Institution | Osaka Kyoiku University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
鈴木 剛 大阪教育大学, 教育学部, 教授 (10314444)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | ラン科植物 / 四倍体 / GISH法 / 動原体 / CENH3 / メチル化 / ヒストンリン酸化抗体 / BACライブラリー / 4倍体 / フウラン / 花のサイズ / ４倍体 / 減数分裂 / 四価染色体 / バンダ属 / エピジェネティック / GISH / ヒストン修飾 / BACクローン |
|---|
| Outline of Final Research Achievements |
We have elucidated the mechanism of genetic stabilization of tetraploids and amphidiploids from inter- and intra-generic hybrids in the subtribe Aeridinae of tribe Vandae which is monophyletic, mostly epiphytic and comprises horticultural valued genera including Vanda, Neofinetia, Ascocentrum, Rhynchostylis. Epigenetic chromatin dynamics by DNA methylation and histone modification were visualized at the chromosome and DNA level using the GISH and immunological staining method. As the molecular mechanism involved in the stabilization of tetraploid seems to be related to the structure of kinetochores, and as clue to unvovering it has been found by conducting the contruction of BAC library such as Neofinetia falcata and the identification of CENH3.
|
