| Project/Area Number |
25450009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Saga University |
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Principal Investigator |
Anai Toyoaki 佐賀大学, 農学部, 教授 (70261774)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Satoshi 佐賀大学, 農学部, 講師 (40425541)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ダイズ / イソフラボン / MYB / 転写制御 / 変異体 / 代謝工学 / 突然変異体 |
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| Outline of Final Research Achievements |
In this study, we tried to establish a novel metabolic engineering approach to modify the key transcriptional factor, MYB, which coordinately regulate multiple target genes involved in the flavonoid metabolic pathway for modification of isoflavone content in soybean seed.
As a result, we could develop a novel heterologus transcriptional factor evaluation system for target gene promotor via agr-infiltration system of N. Benthamiana, and achieved to identify the MYB gene that regulates a target, CHS7 gene expression.
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