The modification of flavonoid metabolic pathway in soybean which is controlled with duplicated transcription factor genes as the targets.

• Project/Area Number: 25450009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Saga University
• Principal Investigator: Anai Toyoaki 佐賀大学, 農学部, 教授 (70261774)
• Co-Investigator(Kenkyū-buntansha): WATANABE Satoshi 佐賀大学, 農学部, 講師 (40425541)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ダイズ / イソフラボン / MYB / 転写制御 / 変異体 / 代謝工学 / 突然変異体

Outline of Final Research Achievements

In this study, we tried to establish a novel metabolic engineering approach to modify the key transcriptional factor, MYB, which coordinately regulate multiple target genes involved in the flavonoid metabolic pathway for modification of isoflavone content in soybean seed.
As a result, we could develop a novel heterologus transcriptional factor evaluation system for target gene promotor via agr-infiltration system of N. Benthamiana, and achieved to identify the MYB gene that regulates a target, CHS7 gene expression.

Research Products

• [Journal Article] Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing. (2015)
  • Author(s): M. Tsuda, A. Kaga, T. Anai, T. Shimizu, T. Sayama, K. Takagi, K. Machita, S. Watanabe, M. Nishimura, N. Yamada, S. Mori, H. Sasaki, H. Kanamori, Y. Katayose, M. Ishimoto
  • Journal Title: BMC Genomics Volume: 16 Issue: 1 Pages: 1014-1014
  • DOI: 10.1186/s12864-015-2079-y
  • Peer Reviewed / Open Access
• [Journal Article] Red/far red light controls arbuscular mycorrhizal colonization via jasmonic acid and strigolactone signaling (2015)
  • Author(s): M. Nagata, N. Yamamoto, T. Shigeyama, Y. Terasawa, T. Anai, T. Sakai, S. Inada, S. Arima, A.M. Hirsch , A. Suzuki
  • Journal Title: Plant and Cell Physiology Volume: 56 Pages: 2100-2109
  • DOI: 10.1093/pcp/pcv135
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] GmCOL1a and GmCOL1b function as flowering repressor in soybean under long-day conditions. (2015)
  • Author(s): D. Cao, Y. Li, S. Lu, J. Wang, H. Nan, X. Li, D. Shi, C. Fang, H. Zhai, X. Yuan, T. Anai, Z. Xia, B. Liu and F. Kong
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 12 Pages: 2409-2422
  • DOI: 10.1093/pcp/pcv152
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel <i>GmFAD3-2a</i> mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil (2014)
  • Author(s): Tomoki Hoshino, Satoshi Watanabe, Yutaka Takagi, Toyoaki Anai
  • Journal Title: Breeding Science Volume: 64 Issue: 4 Pages: 371-377
  • DOI: 10.1270/jsbbs.64.371
  • NAID: 130004888870
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2 (2014)
  • Author(s): F. Yan, S. Di, F. R. Rodas, T. R. Torrico, Y. Murai, T. Iwashina, T. Anai and R. Takahashi
  • Journal Title: BMC Plant Biology Volume: 14 Issue: 1 Pages: 58-58
  • DOI: 10.1186/1471-2229-14-58
  • Peer Reviewed
• [Presentation] ダイズのイソフラボン生合成を制御するMYB転写因子の探索 (2015)
  • Author(s): 弥吉俊樹、渡邉啓史、穴井豊昭
  • Organizer: 第10回 九州育種談話会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2015-08-27
• [Book] Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools (2015)
  • Author(s): Toyoaki Anai
  • Total Pages: 15
  • Publisher: Springer International Publishing AG