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Transcriptome analysis of buckwheat stamens to identify the genes involved in heteromorphic self-incompatibility

Research Project

Project/Area Number 25450011
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionNigata University of Phermacy and Applied Life Sciences

Principal Investigator

AII JOTARO  新潟薬科大学, 応用生命科学部, 准教授 (10391591)

Co-Investigator(Kenkyū-buntansha) 森 正之  石川県立大学, 生物資源環境学部, 准教授 (00320911)
大田 竜也  総合研究大学院大学, 先導科学研究科, 准教授 (30322100)
Co-Investigator(Renkei-kenkyūsha) YASUI YASUO  京都大学, (連合)農学研究科(研究院), 助教 (70293917)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords自家不和合性 / 生殖様式 / 異形花型自家不和合性 / ソバ / トランスクリプトーム解析 / 雄性側因子 / 自家和合性変異体 / 雄性側S遺伝子 / 雄性側S因子 / 自家和合性突然変異体
Outline of Final Research Achievements

Common buckwheat, Fagopyrum esculentum, is a heteromorphic self-incompatibility (SI) species. Its yield is relatively unstable compared to other major self-fertilizing crops due to heteromorphic SI. In this study, we attempted to identify the genes involved in heteromorphic SI of buckwheat. Noble two genes were found by transcriptome and genomic analyses, which were located to the S locus and to be expressed in the short-styled flower. These two genes are suitable candidate genes for the control of the short-styled phenotype of buckwheat plants. Our knowledge obtained from this study will enable to develop self-fertilizing buckwheat cultivars.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2016 2014 2013 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Assembly of the draft genome of buckwheat and its applications in identifying agronomically useful genes2016

    • Author(s)
      Yasuo Yasui, Hideki Hirakawa, Mariko Ueno, Katsuhiro Matsui, Tomoyuki Katsube-Tanaka, Soo Jung Yang, Jotaro Aii, Singo Sato and Mavashi Mori
    • Journal Title

      DNA Research

      Volume: 2 Issue: 3 Pages: 1-10

    • DOI

      10.1093/dnares/dsw012

    • NAID

      120005743643

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 新規な自家不和合性機構解明への挑戦2014

    • Author(s)
      掛田克行、佐々英徳、土屋亨、相井城太郎
    • Journal Title

      育種学研究

      Volume: 16 Pages: 53-60

    • NAID

      130004438687

    • Related Report
      2014 Research-status Report
  • [Presentation] 異形花型自家不和合性を示すソバの雄蕊におけるトランスクリプトーム解析2016

    • Author(s)
      相井城太郎、大田竜也、安井康夫、森正之、佐藤真吾、田巻茜、中野絢菜、キャンベル・クレイトン、長野美緒、田中宥司
    • Organizer
      日本育種学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] RNA-seq解析によるソバ異形花型自家不和合性の雄性因子の探索2014

    • Author(s)
      相井城太郎、佐藤真吾、田巻茜、長野美緒、キャンベル クレイトン、安井康夫、森正之、大田竜也、田中宥司
    • Organizer
      日本育種学会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ソバの異形花型自家不和合性機構2013

    • Author(s)
      相井城太郎、佐藤真吾、佐藤祐也、中野絢菜、田中宥司
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Intact S-ELF3 is exclusive to heteromorphic SI species in Fagopyrum2013

    • Author(s)
      Yasuo Yasui, Masashi Mori, Jotaro Aii, Shingo Sato, Ohmi Ohnishi, Tomoko Abe, Yoriko Hayashi, Daiki Matsumoto and Tastuya Ota
    • Organizer
      第12回国際ソバシンポジウム
    • Place of Presentation
      スロヴェニア ラシュコ
    • Related Report
      2013 Research-status Report
  • [Remarks] 世界初となるソバの全ゲノム解読に成功

    • URL

      http://www.nupals.ac.jp/news/2016/04/post-197.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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